Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.

<h4>Introduction</h4>Aneurysms of the ascending aorta (AA) correspond to a dilatation of the ascending aorta that progressively evolves over several years. The main complication of aneurysms of the ascending aorta is type A aortic dissection, which is associated with very high rates of m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marie-Catherine Morgant, Siyu Lin, Diana Marin-Castrillon, Chloé Bernard, Aline Laubriet, Alexandre Cochet, Alain Lalande, Olivier Bouchot
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ee906ad8b6447cabaf19fe648df5a39
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0ee906ad8b6447cabaf19fe648df5a39
record_format	dspace
spelling	oai:doaj.org-article:0ee906ad8b6447cabaf19fe648df5a392021-12-02T20:08:17ZComparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.1932-620310.1371/journal.pone.0256278https://doaj.org/article/0ee906ad8b6447cabaf19fe648df5a392021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256278https://doaj.org/toc/1932-6203<h4>Introduction</h4>Aneurysms of the ascending aorta (AA) correspond to a dilatation of the ascending aorta that progressively evolves over several years. The main complication of aneurysms of the ascending aorta is type A aortic dissection, which is associated with very high rates of morbidity and mortality. Prophylactic ascending aorta replacement guidelines are currently based on maximal AA diameter. However, this criterion is imperfect. Stretching tests on the aorta carried out ex-vivo make it possible to determine the elastic properties of healthy and aneurysmal aortic fragments (tension test, resistance before rupture). For several years now, cardiac magnetic resonance imaging (MRI) has provided another means of evaluating the elastic properties of the aorta. This imaging technique has the advantage of being non-invasive and of establishing aortic compliance (local measurement of stiffness) without using contrast material by measuring the variation of the aortic surface area during the cardiac cycle, and pulse wave velocity (overall stiffness of the aorta).<h4>Materials and methods</h4>Prospective single-center study including 100 patients with ascending aortic aneurysm requiring surgery. We will perform preoperative cine-MRI and biomechanical laboratory stretching tests on aortic samples collected during the cardiac procedure. Images will be acquired with a 3T MRI with only four other acquisitions in addition to the conventional protocol. These additional sequences are a Fast Low Angle Shot (FLASH)-type sequence performed during a short breath-hold in the transverse plane at the level of the bifurcation of the pulmonary artery, and phase-contrast sequences that encodes velocity at the same localization, and also in planes perpendicular to the aorta at the levels of the sino-tubular junction and the diaphragm for the descending aorta. For ex-vivo tests, the experiments will be carried out by a biaxial tensile test machine (ElectroForce®). Each specimen will be stretched with 10 times of 10% preconditioning and at a rate of 10 mm/min until rupture. During the experiment, the tissue is treated under a 37°C saline bath. The maximum elastic modulus from each sample will be calculated.<h4>Results</h4>The aim of this study is to obtain local patient-specific elastic modulus distribution of the ascending aorta from biaxial tensile tests and to assess elastic properties of the aorta using MRI, then to evaluate the correlation between biaxial tests and MRI measurements.<h4>Discussion</h4>Our research hypothesis is that there is a correlation between the evaluation of the elastic properties of the aorta from cardiac MRI and from stretching tests performed ex-vivo on aorta samples collected during ascending aorta replacement.Marie-Catherine MorgantSiyu LinDiana Marin-CastrillonChloé BernardAline LaubrietAlexandre CochetAlain LalandeOlivier BouchotPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256278 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marie-Catherine Morgant Siyu Lin Diana Marin-Castrillon Chloé Bernard Aline Laubriet Alexandre Cochet Alain Lalande Olivier Bouchot Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.
description	<h4>Introduction</h4>Aneurysms of the ascending aorta (AA) correspond to a dilatation of the ascending aorta that progressively evolves over several years. The main complication of aneurysms of the ascending aorta is type A aortic dissection, which is associated with very high rates of morbidity and mortality. Prophylactic ascending aorta replacement guidelines are currently based on maximal AA diameter. However, this criterion is imperfect. Stretching tests on the aorta carried out ex-vivo make it possible to determine the elastic properties of healthy and aneurysmal aortic fragments (tension test, resistance before rupture). For several years now, cardiac magnetic resonance imaging (MRI) has provided another means of evaluating the elastic properties of the aorta. This imaging technique has the advantage of being non-invasive and of establishing aortic compliance (local measurement of stiffness) without using contrast material by measuring the variation of the aortic surface area during the cardiac cycle, and pulse wave velocity (overall stiffness of the aorta).<h4>Materials and methods</h4>Prospective single-center study including 100 patients with ascending aortic aneurysm requiring surgery. We will perform preoperative cine-MRI and biomechanical laboratory stretching tests on aortic samples collected during the cardiac procedure. Images will be acquired with a 3T MRI with only four other acquisitions in addition to the conventional protocol. These additional sequences are a Fast Low Angle Shot (FLASH)-type sequence performed during a short breath-hold in the transverse plane at the level of the bifurcation of the pulmonary artery, and phase-contrast sequences that encodes velocity at the same localization, and also in planes perpendicular to the aorta at the levels of the sino-tubular junction and the diaphragm for the descending aorta. For ex-vivo tests, the experiments will be carried out by a biaxial tensile test machine (ElectroForce®). Each specimen will be stretched with 10 times of 10% preconditioning and at a rate of 10 mm/min until rupture. During the experiment, the tissue is treated under a 37°C saline bath. The maximum elastic modulus from each sample will be calculated.<h4>Results</h4>The aim of this study is to obtain local patient-specific elastic modulus distribution of the ascending aorta from biaxial tensile tests and to assess elastic properties of the aorta using MRI, then to evaluate the correlation between biaxial tests and MRI measurements.<h4>Discussion</h4>Our research hypothesis is that there is a correlation between the evaluation of the elastic properties of the aorta from cardiac MRI and from stretching tests performed ex-vivo on aorta samples collected during ascending aorta replacement.
format	article
author	Marie-Catherine Morgant Siyu Lin Diana Marin-Castrillon Chloé Bernard Aline Laubriet Alexandre Cochet Alain Lalande Olivier Bouchot
author_facet	Marie-Catherine Morgant Siyu Lin Diana Marin-Castrillon Chloé Bernard Aline Laubriet Alexandre Cochet Alain Lalande Olivier Bouchot
author_sort	Marie-Catherine Morgant
title	Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.
title_short	Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.
title_full	Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.
title_fullStr	Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.
title_full_unstemmed	Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.
title_sort	comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: prospective cohort study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0ee906ad8b6447cabaf19fe648df5a39
work_keys_str_mv	AT mariecatherinemorgant comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT siyulin comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT dianamarincastrillon comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT chloebernard comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT alinelaubriet comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT alexandrecochet comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT alainlalande comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy AT olivierbouchot comparisonoftwotechniquesinvivoandexvivoforevaluatingtheelasticpropertiesoftheascendingaortaprospectivecohortstudy
_version_	1718375164248326144

Comparison of two techniques (in vivo and ex-vivo) for evaluating the elastic properties of the ascending aorta: Prospective cohort study.

Ejemplares similares