The Combined Effect of Pseudomonas stutzeri and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (Lactuca sativa) to Cadmium Stress

Agricultural activities lead to the accumulation of cadmium (Cd) in the soil. It is necessary to identify effective and economical ways to reduce the soil Cd bioavailability. To achieve this, three bacterial strains, Pseudomonas stutzeri, P. koreensis, and <i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohssen Elbagory, Diaa K. Farrag, Ahmed M. Hashim, Alaa El-Dein Omara
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cadmium biochar <i>Pseudomonas stutzeri</i> bioavailability lettuce Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0eed56ac862242908943e16190ba8e0e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0eed56ac862242908943e16190ba8e0e
record_format	dspace
spelling	oai:doaj.org-article:0eed56ac862242908943e16190ba8e0e2021-11-25T17:47:03ZThe Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress10.3390/horticulturae71104302311-7524https://doaj.org/article/0eed56ac862242908943e16190ba8e0e2021-10-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/430https://doaj.org/toc/2311-7524Agricultural activities lead to the accumulation of cadmium (Cd) in the soil. It is necessary to identify effective and economical ways to reduce the soil Cd bioavailability. To achieve this, three bacterial strains, <i>Pseudomonas stutzeri</i>, <i>P. koreensis</i>, and <i>P. fluorescens</i>, were tested for tolerance and biosorption of different concentrations of Cd (0, 5, 10, 15, 20, and 25 mg L<sup>−1</sup>). During the 2020 and 2021 seasons, a pot experiment was conducted using four different soil amendments (control, biochar, <i>P. stutzeri</i>, and a combination) under four levels of Cd (0, 40, 80, and 120 mg kg<sup>−1</sup>) and assessing the effect on growth parameters, physiological modifications, antioxidant enzymes, and Cd accumulation in lettuce plants (<i>Lactuca sativa</i> cv. <i>Balady</i>). In vitro, the results showed that <i>P. stutzeri</i> was the most tolerant of Cd. Our findings in pot trials showed that T4 (biochar + <i>P. stutzeri</i>) was a more efficient treatment in terms of the growth parameters, with 452.00 g plant<sup>−1</sup> was recorded for fresh weight, 40.10 g plant<sup>−1</sup> for dry weight, 18.89 cm plant<sup>−1</sup> for plant height, 6.03 cm<sup>2</sup> for leaf area, and 20.48 for the number of leaves plant<sup>−1</sup>, while in terms of physiological characteristics, we recorded 1.29 mg g<sup>−1</sup> FW, 0.35 μg g<sup>−1</sup> FW, and 3.69 μg g<sup>−1</sup> FW for total chlorophyll, carotenoids, and total soluble sugar, respectively; this was also reflected in the number of antioxidant enzymes and intensity of soil biological activities in soil treated with 120 mg kg<sup>−1</sup> Cd compared with the control and other treatments in the first season. A similar trend was observed in the second season. Additionally, significantly lower Cd was observed in both the root (67%) and shoots (78%). Therefore, a combined application of biochar and <i>P. stutzeri</i> could be used as an alternative to mitigate Cd toxicity.Mohssen ElbagoryDiaa K. FarragAhmed M. HashimAlaa El-Dein OmaraMDPI AGarticlecadmiumbiochar<i>Pseudomonas stutzeri</i>bioavailabilitylettucePlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 430, p 430 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cadmium biochar <i>Pseudomonas stutzeri</i> bioavailability lettuce Plant culture SB1-1110
spellingShingle	cadmium biochar <i>Pseudomonas stutzeri</i> bioavailability lettuce Plant culture SB1-1110 Mohssen Elbagory Diaa K. Farrag Ahmed M. Hashim Alaa El-Dein Omara The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress
description	Agricultural activities lead to the accumulation of cadmium (Cd) in the soil. It is necessary to identify effective and economical ways to reduce the soil Cd bioavailability. To achieve this, three bacterial strains, <i>Pseudomonas stutzeri</i>, <i>P. koreensis</i>, and <i>P. fluorescens</i>, were tested for tolerance and biosorption of different concentrations of Cd (0, 5, 10, 15, 20, and 25 mg L<sup>−1</sup>). During the 2020 and 2021 seasons, a pot experiment was conducted using four different soil amendments (control, biochar, <i>P. stutzeri</i>, and a combination) under four levels of Cd (0, 40, 80, and 120 mg kg<sup>−1</sup>) and assessing the effect on growth parameters, physiological modifications, antioxidant enzymes, and Cd accumulation in lettuce plants (<i>Lactuca sativa</i> cv. <i>Balady</i>). In vitro, the results showed that <i>P. stutzeri</i> was the most tolerant of Cd. Our findings in pot trials showed that T4 (biochar + <i>P. stutzeri</i>) was a more efficient treatment in terms of the growth parameters, with 452.00 g plant<sup>−1</sup> was recorded for fresh weight, 40.10 g plant<sup>−1</sup> for dry weight, 18.89 cm plant<sup>−1</sup> for plant height, 6.03 cm<sup>2</sup> for leaf area, and 20.48 for the number of leaves plant<sup>−1</sup>, while in terms of physiological characteristics, we recorded 1.29 mg g<sup>−1</sup> FW, 0.35 μg g<sup>−1</sup> FW, and 3.69 μg g<sup>−1</sup> FW for total chlorophyll, carotenoids, and total soluble sugar, respectively; this was also reflected in the number of antioxidant enzymes and intensity of soil biological activities in soil treated with 120 mg kg<sup>−1</sup> Cd compared with the control and other treatments in the first season. A similar trend was observed in the second season. Additionally, significantly lower Cd was observed in both the root (67%) and shoots (78%). Therefore, a combined application of biochar and <i>P. stutzeri</i> could be used as an alternative to mitigate Cd toxicity.
format	article
author	Mohssen Elbagory Diaa K. Farrag Ahmed M. Hashim Alaa El-Dein Omara
author_facet	Mohssen Elbagory Diaa K. Farrag Ahmed M. Hashim Alaa El-Dein Omara
author_sort	Mohssen Elbagory
title	The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress
title_short	The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress
title_full	The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress
title_fullStr	The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress
title_full_unstemmed	The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress
title_sort	combined effect of <i>pseudomonas stutzeri</i> and biochar on the growth dynamics and tolerance of lettuce plants (<i>lactuca sativa</i>) to cadmium stress
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0eed56ac862242908943e16190ba8e0e
work_keys_str_mv	AT mohssenelbagory thecombinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT diaakfarrag thecombinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT ahmedmhashim thecombinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT alaaeldeinomara thecombinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT mohssenelbagory combinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT diaakfarrag combinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT ahmedmhashim combinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress AT alaaeldeinomara combinedeffectofipseudomonasstutzeriiandbiocharonthegrowthdynamicsandtoleranceoflettuceplantsilactucasativaitocadmiumstress
_version_	1718412027699920896

The Combined Effect of <i>Pseudomonas stutzeri</i> and Biochar on the Growth Dynamics and Tolerance of Lettuce Plants (<i>Lactuca sativa</i>) to Cadmium Stress

Ejemplares similares