An alternative food source for metabolism and longevity studies in Caenorhabditis elegans

Beydoun, Choi et al. develop a metabolically inactive food source for C. elegans worms that minimizes the confounding effects of bacterial metabolism on worm metabolism and aging. They find that worms eat and grow well on a paraformaldehyde (PFA)-treated E. coli food source, recommending the use of...

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Autores principales: Safa Beydoun, Hyo Sub Choi, Gabrielle Dela-Cruz, Joseph Kruempel, Shijiao Huang, Daphne Bazopoulou, Hillary A. Miller, Megan L. Schaller, Charles R. Evans, Scott F. Leiser
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/0eeff3968ef44f02a9f5c443b0a3f8f5
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spelling oai:doaj.org-article:0eeff3968ef44f02a9f5c443b0a3f8f52021-12-02T13:20:00ZAn alternative food source for metabolism and longevity studies in Caenorhabditis elegans10.1038/s42003-021-01764-42399-3642https://doaj.org/article/0eeff3968ef44f02a9f5c443b0a3f8f52021-02-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01764-4https://doaj.org/toc/2399-3642Beydoun, Choi et al. develop a metabolically inactive food source for C. elegans worms that minimizes the confounding effects of bacterial metabolism on worm metabolism and aging. They find that worms eat and grow well on a paraformaldehyde (PFA)-treated E. coli food source, recommending the use of 0.5% PFA-killed bacteria as a nematode food source for metabolic experiments.Safa BeydounHyo Sub ChoiGabrielle Dela-CruzJoseph KruempelShijiao HuangDaphne BazopoulouHillary A. MillerMegan L. SchallerCharles R. EvansScott F. LeiserNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Safa Beydoun
Hyo Sub Choi
Gabrielle Dela-Cruz
Joseph Kruempel
Shijiao Huang
Daphne Bazopoulou
Hillary A. Miller
Megan L. Schaller
Charles R. Evans
Scott F. Leiser
An alternative food source for metabolism and longevity studies in Caenorhabditis elegans
description Beydoun, Choi et al. develop a metabolically inactive food source for C. elegans worms that minimizes the confounding effects of bacterial metabolism on worm metabolism and aging. They find that worms eat and grow well on a paraformaldehyde (PFA)-treated E. coli food source, recommending the use of 0.5% PFA-killed bacteria as a nematode food source for metabolic experiments.
format article
author Safa Beydoun
Hyo Sub Choi
Gabrielle Dela-Cruz
Joseph Kruempel
Shijiao Huang
Daphne Bazopoulou
Hillary A. Miller
Megan L. Schaller
Charles R. Evans
Scott F. Leiser
author_facet Safa Beydoun
Hyo Sub Choi
Gabrielle Dela-Cruz
Joseph Kruempel
Shijiao Huang
Daphne Bazopoulou
Hillary A. Miller
Megan L. Schaller
Charles R. Evans
Scott F. Leiser
author_sort Safa Beydoun
title An alternative food source for metabolism and longevity studies in Caenorhabditis elegans
title_short An alternative food source for metabolism and longevity studies in Caenorhabditis elegans
title_full An alternative food source for metabolism and longevity studies in Caenorhabditis elegans
title_fullStr An alternative food source for metabolism and longevity studies in Caenorhabditis elegans
title_full_unstemmed An alternative food source for metabolism and longevity studies in Caenorhabditis elegans
title_sort alternative food source for metabolism and longevity studies in caenorhabditis elegans
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/0eeff3968ef44f02a9f5c443b0a3f8f5
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