Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor

Abstract Trimethylamine N-oxide (TMAO), a microbiota-derived metabolite has been implicated in human health and disease. Its early detection in body fluids has been presumed to be significant in understanding the pathogenesis and treatment of many diseases. Hence, the development of reliable and rap...

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Autores principales:	G. B. V. S. Lakshmi, Amit K. Yadav, Neha Mehlawat, Rekha Jalandra, Pratima R. Solanki, Anil Kumar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ef667f6caf64b18b959fe794abe850e
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spelling	oai:doaj.org-article:0ef667f6caf64b18b959fe794abe850e2021-12-02T15:23:00ZGut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor10.1038/s41598-020-80122-62045-2322https://doaj.org/article/0ef667f6caf64b18b959fe794abe850e2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80122-6https://doaj.org/toc/2045-2322Abstract Trimethylamine N-oxide (TMAO), a microbiota-derived metabolite has been implicated in human health and disease. Its early detection in body fluids has been presumed to be significant in understanding the pathogenesis and treatment of many diseases. Hence, the development of reliable and rapid technologies for TMAO detection may augment our understanding of pathogenesis and diagnosis of diseases that TMAO has implicated. The present work is the first report on the development of a molecularly imprinted polymer (MIP) based electrochemical sensor for sensitive and selective detection of TMAO in body fluids. The MIP developed was based on the polypyrrole (PPy), which was synthesized via chemical oxidation polymerization method, with and without the presence of TMAO. The MIP, NIP and the non-sonicated polymer (PPy-TMAO) were separately deposited electrophoretically onto the hydrolyzed indium tin oxide (ITO) coated glasses. The chemical, morphological, and electrochemical behavior of MIP, non-imprinted polymer (NIP), and PPy-TMAO were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and electrochemical techniques. The detection response was recorded using differential pulse voltammetry (DPV), which revealed a decrease in the peak current with the increase in concentration of TMAO. The MIP sensor showed a dynamic detection range of 1–15 ppm with a sensitivity of 2.47 µA mL ppm−1 cm−2. The developed sensor is easy to construct and operate and is also highly selective to detect TMAO in body fluids such as urine. The present research provides a basis for innovative strategies to develop sensors based on MIP to detect other metabolites derived from gut microbiota that are implicated in human health and diseases.G. B. V. S. LakshmiAmit K. YadavNeha MehlawatRekha JalandraPratima R. SolankiAnil KumarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q G. B. V. S. Lakshmi Amit K. Yadav Neha Mehlawat Rekha Jalandra Pratima R. Solanki Anil Kumar Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor
description	Abstract Trimethylamine N-oxide (TMAO), a microbiota-derived metabolite has been implicated in human health and disease. Its early detection in body fluids has been presumed to be significant in understanding the pathogenesis and treatment of many diseases. Hence, the development of reliable and rapid technologies for TMAO detection may augment our understanding of pathogenesis and diagnosis of diseases that TMAO has implicated. The present work is the first report on the development of a molecularly imprinted polymer (MIP) based electrochemical sensor for sensitive and selective detection of TMAO in body fluids. The MIP developed was based on the polypyrrole (PPy), which was synthesized via chemical oxidation polymerization method, with and without the presence of TMAO. The MIP, NIP and the non-sonicated polymer (PPy-TMAO) were separately deposited electrophoretically onto the hydrolyzed indium tin oxide (ITO) coated glasses. The chemical, morphological, and electrochemical behavior of MIP, non-imprinted polymer (NIP), and PPy-TMAO were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and electrochemical techniques. The detection response was recorded using differential pulse voltammetry (DPV), which revealed a decrease in the peak current with the increase in concentration of TMAO. The MIP sensor showed a dynamic detection range of 1–15 ppm with a sensitivity of 2.47 µA mL ppm−1 cm−2. The developed sensor is easy to construct and operate and is also highly selective to detect TMAO in body fluids such as urine. The present research provides a basis for innovative strategies to develop sensors based on MIP to detect other metabolites derived from gut microbiota that are implicated in human health and diseases.
format	article
author	G. B. V. S. Lakshmi Amit K. Yadav Neha Mehlawat Rekha Jalandra Pratima R. Solanki Anil Kumar
author_facet	G. B. V. S. Lakshmi Amit K. Yadav Neha Mehlawat Rekha Jalandra Pratima R. Solanki Anil Kumar
author_sort	G. B. V. S. Lakshmi
title	Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor
title_short	Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor
title_full	Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor
title_fullStr	Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor
title_full_unstemmed	Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor
title_sort	gut microbiota derived trimethylamine n-oxide (tmao) detection through molecularly imprinted polymer based sensor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0ef667f6caf64b18b959fe794abe850e
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Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor

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