Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.

Despite an ongoing focus on the role of diet in health and disease, we have only a limited understanding of these concepts at the cellular and molecular levels. While obesity has been clearly recognized as contributing to metabolic syndrome and the pathogenesis of adult asthma, recent evidence has l...

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Autores principales:	Caroline M Percopo, Morgan McCullough, Ajinkya R Limkar, Kirk M Druey, Helene F Rosenberg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0ef99e27a7b54af194a477968066ac0d
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spelling	oai:doaj.org-article:0ef99e27a7b54af194a477968066ac0d2021-12-02T20:18:15ZImpact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.1932-620310.1371/journal.pone.0255997https://doaj.org/article/0ef99e27a7b54af194a477968066ac0d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255997https://doaj.org/toc/1932-6203Despite an ongoing focus on the role of diet in health and disease, we have only a limited understanding of these concepts at the cellular and molecular levels. While obesity has been clearly recognized as contributing to metabolic syndrome and the pathogenesis of adult asthma, recent evidence has linked high sugar intake alone to an increased risk of developing asthma in childhood. In this study, we examined the impact of diet in a mouse model of allergic airways inflammation with a specific focus on eosinophils. As anticipated, male C57BL/6 mice gained weight on a high-calorie, high-fat diet. However, mice also gained weight on an isocaloric high-sucrose diet. Elevated levels of leptin were detected in the serum and airways of mice maintained on the high-fat, but not the high-sucrose diets. We found that diet alone had no impact on eosinophil numbers in the airways at baseline or their recruitment in response to allergen (Alternaria alternata) challenge in either wild-type or leptin-deficient ob/ob mice. However, both bronchoalveolar lavage fluid and eosinophils isolated from lung tissue of allergen-challenged mice exhibited profound diet-dependent differences in cytokine content. Similarly, while all wild-type mice responded to allergen challenge with significant increases in methacholine-dependent total airway resistance (Rrs), airway resistance in mice maintained on the isocaloric high-sucrose (but not the high-calorie/high-fat) diet significantly exceeded that of mice maintained on the basic diet. In summary, our findings revealed that mice maintained on an isocaloric high-sucrose diet responded to allergen challenge with significant changes in both BAL and eosinophil cytokine content together with significant increases in Rrs. These results provide a model for further exploration of the unique risks associated with a high-sugar diet and its impact on allergen-associated respiratory dysfunction.Caroline M PercopoMorgan McCulloughAjinkya R LimkarKirk M DrueyHelene F RosenbergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255997 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Caroline M Percopo Morgan McCullough Ajinkya R Limkar Kirk M Druey Helene F Rosenberg Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
description	Despite an ongoing focus on the role of diet in health and disease, we have only a limited understanding of these concepts at the cellular and molecular levels. While obesity has been clearly recognized as contributing to metabolic syndrome and the pathogenesis of adult asthma, recent evidence has linked high sugar intake alone to an increased risk of developing asthma in childhood. In this study, we examined the impact of diet in a mouse model of allergic airways inflammation with a specific focus on eosinophils. As anticipated, male C57BL/6 mice gained weight on a high-calorie, high-fat diet. However, mice also gained weight on an isocaloric high-sucrose diet. Elevated levels of leptin were detected in the serum and airways of mice maintained on the high-fat, but not the high-sucrose diets. We found that diet alone had no impact on eosinophil numbers in the airways at baseline or their recruitment in response to allergen (Alternaria alternata) challenge in either wild-type or leptin-deficient ob/ob mice. However, both bronchoalveolar lavage fluid and eosinophils isolated from lung tissue of allergen-challenged mice exhibited profound diet-dependent differences in cytokine content. Similarly, while all wild-type mice responded to allergen challenge with significant increases in methacholine-dependent total airway resistance (Rrs), airway resistance in mice maintained on the isocaloric high-sucrose (but not the high-calorie/high-fat) diet significantly exceeded that of mice maintained on the basic diet. In summary, our findings revealed that mice maintained on an isocaloric high-sucrose diet responded to allergen challenge with significant changes in both BAL and eosinophil cytokine content together with significant increases in Rrs. These results provide a model for further exploration of the unique risks associated with a high-sugar diet and its impact on allergen-associated respiratory dysfunction.
format	article
author	Caroline M Percopo Morgan McCullough Ajinkya R Limkar Kirk M Druey Helene F Rosenberg
author_facet	Caroline M Percopo Morgan McCullough Ajinkya R Limkar Kirk M Druey Helene F Rosenberg
author_sort	Caroline M Percopo
title	Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
title_short	Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
title_full	Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
title_fullStr	Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
title_full_unstemmed	Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
title_sort	impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0ef99e27a7b54af194a477968066ac0d
work_keys_str_mv	AT carolinempercopo impactofcontrolledhighsucroseandhighfatdietsoneosinophilrecruitmentandcytokinecontentinallergenchallengedmice AT morganmccullough impactofcontrolledhighsucroseandhighfatdietsoneosinophilrecruitmentandcytokinecontentinallergenchallengedmice AT ajinkyarlimkar impactofcontrolledhighsucroseandhighfatdietsoneosinophilrecruitmentandcytokinecontentinallergenchallengedmice AT kirkmdruey impactofcontrolledhighsucroseandhighfatdietsoneosinophilrecruitmentandcytokinecontentinallergenchallengedmice AT helenefrosenberg impactofcontrolledhighsucroseandhighfatdietsoneosinophilrecruitmentandcytokinecontentinallergenchallengedmice
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Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice.

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