Optogenetic control of protein binding using light-switchable nanobodies

The ability to regulate nanobody affinity with light would expand the applications toolbox for these reagents. Here the authors insert an optimised photoswitchable AsLOV2 domain into multiple nanobodies and demonstrate photoswitchable binding to fluorescent proteins and endogenous proteins in cells.

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Autores principales:	Agnieszka A. Gil, César Carrasco-López, Liyuan Zhu, Evan M. Zhao, Pavithran T. Ravindran, Maxwell Z. Wilson, Alexander G. Goglia, José L. Avalos, Jared E. Toettcher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0efe65320d7b472997bd875d0dcd862e
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spelling	oai:doaj.org-article:0efe65320d7b472997bd875d0dcd862e2021-12-02T15:08:24ZOptogenetic control of protein binding using light-switchable nanobodies10.1038/s41467-020-17836-82041-1723https://doaj.org/article/0efe65320d7b472997bd875d0dcd862e2020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17836-8https://doaj.org/toc/2041-1723The ability to regulate nanobody affinity with light would expand the applications toolbox for these reagents. Here the authors insert an optimised photoswitchable AsLOV2 domain into multiple nanobodies and demonstrate photoswitchable binding to fluorescent proteins and endogenous proteins in cells.Agnieszka A. GilCésar Carrasco-LópezLiyuan ZhuEvan M. ZhaoPavithran T. RavindranMaxwell Z. WilsonAlexander G. GogliaJosé L. AvalosJared E. ToettcherNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Agnieszka A. Gil César Carrasco-López Liyuan Zhu Evan M. Zhao Pavithran T. Ravindran Maxwell Z. Wilson Alexander G. Goglia José L. Avalos Jared E. Toettcher Optogenetic control of protein binding using light-switchable nanobodies
description	The ability to regulate nanobody affinity with light would expand the applications toolbox for these reagents. Here the authors insert an optimised photoswitchable AsLOV2 domain into multiple nanobodies and demonstrate photoswitchable binding to fluorescent proteins and endogenous proteins in cells.
format	article
author	Agnieszka A. Gil César Carrasco-López Liyuan Zhu Evan M. Zhao Pavithran T. Ravindran Maxwell Z. Wilson Alexander G. Goglia José L. Avalos Jared E. Toettcher
author_facet	Agnieszka A. Gil César Carrasco-López Liyuan Zhu Evan M. Zhao Pavithran T. Ravindran Maxwell Z. Wilson Alexander G. Goglia José L. Avalos Jared E. Toettcher
author_sort	Agnieszka A. Gil
title	Optogenetic control of protein binding using light-switchable nanobodies
title_short	Optogenetic control of protein binding using light-switchable nanobodies
title_full	Optogenetic control of protein binding using light-switchable nanobodies
title_fullStr	Optogenetic control of protein binding using light-switchable nanobodies
title_full_unstemmed	Optogenetic control of protein binding using light-switchable nanobodies
title_sort	optogenetic control of protein binding using light-switchable nanobodies
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0efe65320d7b472997bd875d0dcd862e
work_keys_str_mv	AT agnieszkaagil optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT cesarcarrascolopez optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT liyuanzhu optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT evanmzhao optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT pavithrantravindran optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT maxwellzwilson optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT alexanderggoglia optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT joselavalos optogeneticcontrolofproteinbindingusinglightswitchablenanobodies AT jaredetoettcher optogeneticcontrolofproteinbindingusinglightswitchablenanobodies
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Optogenetic control of protein binding using light-switchable nanobodies

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