The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease

Abstract Pine wilt disease (PWD) is a devastating forest disease present worldwide. In this study we analyzed the effects of the invasion of the pinewood nematode Bursaphelenchus xylophilus, the major pathogen causing PWD, on the endophytic microbiome of adult P. pinaster trees. Wood samples from tr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Diogo Neves Proença, Romeu Francisco, Susanne Kublik, Anne Schöler, Gisle Vestergaard, Michael Schloter, Paula V. Morais
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f01979b19e946778a903a2bdf23b409
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0f01979b19e946778a903a2bdf23b409
record_format	dspace
spelling	oai:doaj.org-article:0f01979b19e946778a903a2bdf23b4092021-12-02T12:31:58ZThe Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease10.1038/s41598-017-04141-62045-2322https://doaj.org/article/0f01979b19e946778a903a2bdf23b4092017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04141-6https://doaj.org/toc/2045-2322Abstract Pine wilt disease (PWD) is a devastating forest disease present worldwide. In this study we analyzed the effects of the invasion of the pinewood nematode Bursaphelenchus xylophilus, the major pathogen causing PWD, on the endophytic microbiome of adult P. pinaster trees. Wood samples from trees with different degrees of PWD disease were collected at two sites (A and M) in Portugal. Endophytic bacteria were characterized based on directly extracted DNA by fingerprinting and barcoding using the 16S rRNA gene as marker. Furthermore, cultivation-based approaches were used to obtain isolates of the major taxa to study their ecophysiology. The endophytic microbiome from P. pinaster trees differed significantly between the two sampling sites. Main bacterial OTUs belonged to the Proteobacteria (39% (site M) - 97% (site A)), and Firmicutes (0.70% (site A) - 44% (site M)). However, consequences of the invasion with the pathogen were comparable. Interestingly diversity of wood endophytic bacteria increased with the severity of the diseases, with highest diversity levels observed in in the most affected trees. Our results suggest that in the first stages of the disease, the defence mechanisms of plants are repressed by the pathogen, resulting in a colonization of the wood interior by soil microorganisms.Diogo Neves ProençaRomeu FranciscoSusanne KublikAnne SchölerGisle VestergaardMichael SchloterPaula V. MoraisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diogo Neves Proença Romeu Francisco Susanne Kublik Anne Schöler Gisle Vestergaard Michael Schloter Paula V. Morais The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
description	Abstract Pine wilt disease (PWD) is a devastating forest disease present worldwide. In this study we analyzed the effects of the invasion of the pinewood nematode Bursaphelenchus xylophilus, the major pathogen causing PWD, on the endophytic microbiome of adult P. pinaster trees. Wood samples from trees with different degrees of PWD disease were collected at two sites (A and M) in Portugal. Endophytic bacteria were characterized based on directly extracted DNA by fingerprinting and barcoding using the 16S rRNA gene as marker. Furthermore, cultivation-based approaches were used to obtain isolates of the major taxa to study their ecophysiology. The endophytic microbiome from P. pinaster trees differed significantly between the two sampling sites. Main bacterial OTUs belonged to the Proteobacteria (39% (site M) - 97% (site A)), and Firmicutes (0.70% (site A) - 44% (site M)). However, consequences of the invasion with the pathogen were comparable. Interestingly diversity of wood endophytic bacteria increased with the severity of the diseases, with highest diversity levels observed in in the most affected trees. Our results suggest that in the first stages of the disease, the defence mechanisms of plants are repressed by the pathogen, resulting in a colonization of the wood interior by soil microorganisms.
format	article
author	Diogo Neves Proença Romeu Francisco Susanne Kublik Anne Schöler Gisle Vestergaard Michael Schloter Paula V. Morais
author_facet	Diogo Neves Proença Romeu Francisco Susanne Kublik Anne Schöler Gisle Vestergaard Michael Schloter Paula V. Morais
author_sort	Diogo Neves Proença
title	The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
title_short	The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
title_full	The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
title_fullStr	The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
title_full_unstemmed	The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
title_sort	microbiome of endophytic, wood colonizing bacteria from pine trees as affected by pine wilt disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0f01979b19e946778a903a2bdf23b409
work_keys_str_mv	AT diogonevesproenca themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT romeufrancisco themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT susannekublik themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT annescholer themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT gislevestergaard themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT michaelschloter themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT paulavmorais themicrobiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT diogonevesproenca microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT romeufrancisco microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT susannekublik microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT annescholer microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT gislevestergaard microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT michaelschloter microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease AT paulavmorais microbiomeofendophyticwoodcolonizingbacteriafrompinetreesasaffectedbypinewiltdisease
_version_	1718394200422088704

The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease

Ejemplares similares