UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium

Makiko Saita,1 Takayuki Ikeda,1,2 Masahiro Yamada,1,3 Katsuhiko Kimoto,4 Masaichi Chang-Il Lee,5 Takahiro Ogawa1 1Division of Advanced Prosthodontics, Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA, USA; 2Department of Complete Denture Prosthodontics, N...

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Autores principales:	Saita M, Ikeda T, Yamada M, Kimoto K, Lee MC, Ogawa T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	nanotechnology dental and orthopedic implants superhydrophilic hydrocarbon osseointegration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0f037f3a2cfe452da3dc8b81880c9ed7
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spelling	oai:doaj.org-article:0f037f3a2cfe452da3dc8b81880c9ed72021-12-02T05:36:18ZUV photofunctionalization promotes nano-biomimetic apatite deposition on titanium1178-2013https://doaj.org/article/0f037f3a2cfe452da3dc8b81880c9ed72016-01-01T00:00:00Zhttps://www.dovepress.com/uv-photofunctionalization-promotes-nano-biomimetic-apatite-deposition--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Makiko Saita,1 Takayuki Ikeda,1,2 Masahiro Yamada,1,3 Katsuhiko Kimoto,4 Masaichi Chang-Il Lee,5 Takahiro Ogawa1 1Division of Advanced Prosthodontics, Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA, USA; 2Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Yokosuka, Japan; 3Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan; 4Department of Prosthodontics and Oral Rehabilitation, 5Yokosuka-Shonan Disaster Health Emergency Research Center and ESR Laboratories, Kanagawa Dental University Graduate School of Dentistry, Yokosuka, Japan Background: Although biomimetic apatite coating is a promising way to provide titanium with osteoconductivity, the efficiency and quality of deposition is often poor. Most titanium implants have microscale surface morphology, and an addition of nanoscale features while preserving the micromorphology may provide further biological benefit. Here, we examined the effect of ultraviolet (UV) light treatment of titanium, or photofunctionalization, on the efficacy of biomimetic apatite deposition on titanium and its biological capability.Methods and results: Micro-roughed titanium disks were prepared by acid-etching with sulfuric acid. Micro-roughened disks with or without photofunctionalization (20-minute exposure to UV light) were immersed in simulated body fluid (SBF) for 1 or 5 days. Photofunctionalized titanium disks were superhydrophilic and did not form surface air bubbles when immersed in SBF, whereas non-photofunctionalized disks were hydrophobic and largely covered with air bubbles during immersion. An apatite-related signal was observed by X-ray diffraction on photofunctionalized titanium after 1 day of SBF immersion, which was equivalent to the one observed after 5 days of immersion of control titanium. Scanning electron microscopy revealed nodular apatite deposition in the valleys and at the inclines of micro-roughened structures without affecting the existing micro-configuration. Micro-roughened titanium and apatite-deposited titanium surfaces had similar roughness values. The attachment, spreading, settling, proliferation, and alkaline phosphate activity of bone marrow-derived osteoblasts were promoted on apatite-coated titanium with photofunctionalization.Conclusion: UV-photofunctionalization of titanium enabled faster deposition of nanoscale biomimetic apatite, resulting in the improved biological capability compared to the similarly prepared apatite-deposited titanium without photofunctionalization. Photofunctionalization-assisted biomimetic apatite deposition may be a novel method to effectively enhance micro-roughened titanium surfaces without altering their microscale morphology. Keywords: nanotechnology, dental and orthopedic implants, superhydrophilic, hydrocarbon, osseointegration Saita MIkeda TYamada MKimoto KLee MCOgawa TDove Medical Pressarticlenanotechnologydental and orthopedic implantssuperhydrophilichydrocarbonosseointegrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 223-234 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanotechnology dental and orthopedic implants superhydrophilic hydrocarbon osseointegration Medicine (General) R5-920
spellingShingle	nanotechnology dental and orthopedic implants superhydrophilic hydrocarbon osseointegration Medicine (General) R5-920 Saita M Ikeda T Yamada M Kimoto K Lee MC Ogawa T UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium
description	Makiko Saita,1 Takayuki Ikeda,1,2 Masahiro Yamada,1,3 Katsuhiko Kimoto,4 Masaichi Chang-Il Lee,5 Takahiro Ogawa1 1Division of Advanced Prosthodontics, Weintraub Center for Reconstructive Biotechnology, UCLA School of Dentistry, Los Angeles, CA, USA; 2Department of Complete Denture Prosthodontics, Nihon University School of Dentistry, Yokosuka, Japan; 3Division of Molecular and Regenerative Prosthodontics, Tohoku University Graduate School of Dentistry, Sendai, Miyagi, Japan; 4Department of Prosthodontics and Oral Rehabilitation, 5Yokosuka-Shonan Disaster Health Emergency Research Center and ESR Laboratories, Kanagawa Dental University Graduate School of Dentistry, Yokosuka, Japan Background: Although biomimetic apatite coating is a promising way to provide titanium with osteoconductivity, the efficiency and quality of deposition is often poor. Most titanium implants have microscale surface morphology, and an addition of nanoscale features while preserving the micromorphology may provide further biological benefit. Here, we examined the effect of ultraviolet (UV) light treatment of titanium, or photofunctionalization, on the efficacy of biomimetic apatite deposition on titanium and its biological capability.Methods and results: Micro-roughed titanium disks were prepared by acid-etching with sulfuric acid. Micro-roughened disks with or without photofunctionalization (20-minute exposure to UV light) were immersed in simulated body fluid (SBF) for 1 or 5 days. Photofunctionalized titanium disks were superhydrophilic and did not form surface air bubbles when immersed in SBF, whereas non-photofunctionalized disks were hydrophobic and largely covered with air bubbles during immersion. An apatite-related signal was observed by X-ray diffraction on photofunctionalized titanium after 1 day of SBF immersion, which was equivalent to the one observed after 5 days of immersion of control titanium. Scanning electron microscopy revealed nodular apatite deposition in the valleys and at the inclines of micro-roughened structures without affecting the existing micro-configuration. Micro-roughened titanium and apatite-deposited titanium surfaces had similar roughness values. The attachment, spreading, settling, proliferation, and alkaline phosphate activity of bone marrow-derived osteoblasts were promoted on apatite-coated titanium with photofunctionalization.Conclusion: UV-photofunctionalization of titanium enabled faster deposition of nanoscale biomimetic apatite, resulting in the improved biological capability compared to the similarly prepared apatite-deposited titanium without photofunctionalization. Photofunctionalization-assisted biomimetic apatite deposition may be a novel method to effectively enhance micro-roughened titanium surfaces without altering their microscale morphology. Keywords: nanotechnology, dental and orthopedic implants, superhydrophilic, hydrocarbon, osseointegration
format	article
author	Saita M Ikeda T Yamada M Kimoto K Lee MC Ogawa T
author_facet	Saita M Ikeda T Yamada M Kimoto K Lee MC Ogawa T
author_sort	Saita M
title	UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium
title_short	UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium
title_full	UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium
title_fullStr	UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium
title_full_unstemmed	UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium
title_sort	uv photofunctionalization promotes nano-biomimetic apatite deposition on titanium
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/0f037f3a2cfe452da3dc8b81880c9ed7
work_keys_str_mv	AT saitam uvphotofunctionalizationpromotesnanobiomimeticapatitedepositionontitanium AT ikedat uvphotofunctionalizationpromotesnanobiomimeticapatitedepositionontitanium AT yamadam uvphotofunctionalizationpromotesnanobiomimeticapatitedepositionontitanium AT kimotok uvphotofunctionalizationpromotesnanobiomimeticapatitedepositionontitanium AT leemc uvphotofunctionalizationpromotesnanobiomimeticapatitedepositionontitanium AT ogawat uvphotofunctionalizationpromotesnanobiomimeticapatitedepositionontitanium
_version_	1718400276390477824

UV photofunctionalization promotes nano-biomimetic apatite deposition on titanium

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