[54] Preparation of biopolymer (dextran) and gentamycin blend against multi-drug resistant bacterial infections associated with catheters
Objective: To characterise and investigate the toxicity of biopolymer dextran (from local isolates of Leuconostoc mesenteroides ssp.) and gentamycin blend against multi-drug resistant bacterial infections associated with catheters. Methods: Extraction, purification and characterisation of biopolymer...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/0f0e6c019de14ca8803fec72e129fc36 |
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| Sumario: | Objective: To characterise and investigate the toxicity of biopolymer dextran (from local isolates of Leuconostoc mesenteroides ssp.) and gentamycin blend against multi-drug resistant bacterial infections associated with catheters. Methods: Extraction, purification and characterisation of biopolymer from local isolates of L. mesenteroides were studied. Also the toxicity of the biopolymer was studied by determining the LD50 (dose required to kill half the members of a tested population after a specified test duration) of produced biopolymer. Results: Locally isolated L. mesenteroides had the ability to produce biopolymer. No toxicity of the biopolymer was observed in mice with an LD50 of >2000 mg/kg. The antibacterial effect of the produced biopolymer was studied against pathogenic bacteria that were multi-drug resistant (these bacteria were isolated and cultured from patients with urinary catheters) by determining the minimum inhibitory concentration (MIC) at different concentrations (2–512 mg/mL). Results showed that the biopolymer had an inhibitory effect against pathogenic bacteria with a MIC of 16–64 mg/mL. The anti-biofilm effect of the biopolymer against pathogenic bacteria was studied on the urinary catheters. The results showed the anti-biofilm effect of the biopolymer with biofilm inhibition reached 78%. All isolates were resistant to tetracycline, aztreonam, cefepime, cefotaxime, cefoxitin and gentamicin, whilst they were sensitive to imipenem, all isolates had ability to produce biofilm. The combined effect between antibiotics and biopolymer dextran was investigated against pathogenic bacteria isolated from the catheters. The antibacterial activity of gentamycin was increased in the presence of biopolymer dextran against all isolates. The anti-biofilm effect of the biopolymer dextran and its blender gentamycin was determined alone and as a blend (dextran-gentamycin) using pre-coated catheters. Results showed the biopolymer dextran-gentamycin blend had an anti-biofilm effect in catheters with a biofilm inhibition rate of 85% and 75% against E. coli and S. aureus, respectively. Conclusion: Locally isolated L. mesenteroides had the ability to produce biopolymer. The biopolymer had an inhibitory effect against pathogenic bacteria with a MIC. Biopolymer dextran-gentamycin blend had an anti-biofilm effect in catheters of up to 85%. |
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