Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum

Abstract The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodi...

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Autores principales:	Jung-Eun Kim, Hyejin Nam, Jiyeun Park, Gyung Ja Choi, Yin-Won Lee, Hokyoung Son
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f13bbf48a034e59babef88b74cfb93e
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spelling	oai:doaj.org-article:0f13bbf48a034e59babef88b74cfb93e2021-12-02T16:31:17ZCharacterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum10.1038/s41598-020-61885-42045-2322https://doaj.org/article/0f13bbf48a034e59babef88b74cfb93e2020-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-61885-4https://doaj.org/toc/2045-2322Abstract The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodimers or heterotrimeric complexes that bind to CCAAT sequences within the promoters of target genes and regulate various cellular processes. To date, except Hap complex, CCAAT-binding complex has been rarely reported in fungi. In this study, we characterized two CCAAT-binding transcription factors (Fct1 and Fct2) in the plant pathogenic fungus Fusarium graminearum. Previously, FCT1 and FCT2 were shown to be related to DNA damage response among eight CCAAT-binding transcription factors in F. graminearum. We demonstrate that the nuclear CCAAT-binding complex of F. graminearum has important functions in various fungal developmental processes, not just DNA damage response but virulence and mycotoxin production. Moreover, the results of biochemical and genetic analyses revealed that Fct1 and Fct2 may form a complex and play distinct roles among the eight CCAAT-binding transcription factors encoded by F. graminearum. To the best of our knowledge, the results of this study represent a substantial advancement in our understanding of the molecular mechanisms underlying the functions of CCAAT-binding factors in eukaryotes.Jung-Eun KimHyejin NamJiyeun ParkGyung Ja ChoiYin-Won LeeHokyoung SonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jung-Eun Kim Hyejin Nam Jiyeun Park Gyung Ja Choi Yin-Won Lee Hokyoung Son Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
description	Abstract The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodimers or heterotrimeric complexes that bind to CCAAT sequences within the promoters of target genes and regulate various cellular processes. To date, except Hap complex, CCAAT-binding complex has been rarely reported in fungi. In this study, we characterized two CCAAT-binding transcription factors (Fct1 and Fct2) in the plant pathogenic fungus Fusarium graminearum. Previously, FCT1 and FCT2 were shown to be related to DNA damage response among eight CCAAT-binding transcription factors in F. graminearum. We demonstrate that the nuclear CCAAT-binding complex of F. graminearum has important functions in various fungal developmental processes, not just DNA damage response but virulence and mycotoxin production. Moreover, the results of biochemical and genetic analyses revealed that Fct1 and Fct2 may form a complex and play distinct roles among the eight CCAAT-binding transcription factors encoded by F. graminearum. To the best of our knowledge, the results of this study represent a substantial advancement in our understanding of the molecular mechanisms underlying the functions of CCAAT-binding factors in eukaryotes.
format	article
author	Jung-Eun Kim Hyejin Nam Jiyeun Park Gyung Ja Choi Yin-Won Lee Hokyoung Son
author_facet	Jung-Eun Kim Hyejin Nam Jiyeun Park Gyung Ja Choi Yin-Won Lee Hokyoung Son
author_sort	Jung-Eun Kim
title	Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
title_short	Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
title_full	Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
title_fullStr	Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
title_full_unstemmed	Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
title_sort	characterization of the ccaat-binding transcription factor complex in the plant pathogenic fungus fusarium graminearum
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0f13bbf48a034e59babef88b74cfb93e
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Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum

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