A new class of wheat gliadin genes and proteins.

The utility of mining DNA sequence data to understand the structure and expression of cereal prolamin genes is demonstrated by the identification of a new class of wheat prolamins. This previously unrecognized wheat prolamin class, given the name δ-gliadins, is the most direct ortholog of barley γ3-...

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Autores principales:	Olin D Anderson, Lingli Dong, Naxin Huo, Yong Q Gu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f147d59ab6144478bf445066321d262
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spelling	oai:doaj.org-article:0f147d59ab6144478bf445066321d2622021-11-18T08:04:18ZA new class of wheat gliadin genes and proteins.1932-620310.1371/journal.pone.0052139https://doaj.org/article/0f147d59ab6144478bf445066321d2622012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23284903/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The utility of mining DNA sequence data to understand the structure and expression of cereal prolamin genes is demonstrated by the identification of a new class of wheat prolamins. This previously unrecognized wheat prolamin class, given the name δ-gliadins, is the most direct ortholog of barley γ3-hordeins. Phylogenetic analysis shows that the orthologous δ-gliadins and γ3-hordeins form a distinct prolamin branch that existed separate from the γ-gliadins and γ-hordeins in an ancestral Triticeae prior to the branching of wheat and barley. The expressed δ-gliadins are encoded by a single gene in each of the hexaploid wheat genomes. This single δ-gliadin/γ3-hordein ortholog may be a general feature of the Triticeae tribe since examination of ESTs from three barley cultivars also confirms a single γ3-hordein gene. Analysis of ESTs and cDNAs shows that the genes are expressed in at least five hexaploid wheat cultivars in addition to diploids Triticum monococcum and Aegilops tauschii. The latter two sequences also allow assignment of the δ-gliadin genes to the A and D genomes, respectively, with the third sequence type assumed to be from the B genome. Two wheat cultivars for which there are sufficient ESTs show different patterns of expression, i.e., with cv Chinese Spring expressing the genes from the A and B genomes, while cv Recital has ESTs from the A and D genomes. Genomic sequences of Chinese Spring show that the D genome gene is inactivated by tandem premature stop codons. A fourth δ-gliadin sequence occurs in the D genome of both Chinese Spring and Ae. tauschii, but no ESTs match this sequence and limited genomic sequences indicates a pseudogene containing frame shifts and premature stop codons. Sequencing of BACs covering a 3 Mb region from Ae. tauschii locates the δ-gliadin gene to the complex Gli-1 plus Glu-3 region on chromosome 1.Olin D AndersonLingli DongNaxin HuoYong Q GuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52139 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Olin D Anderson Lingli Dong Naxin Huo Yong Q Gu A new class of wheat gliadin genes and proteins.
description	The utility of mining DNA sequence data to understand the structure and expression of cereal prolamin genes is demonstrated by the identification of a new class of wheat prolamins. This previously unrecognized wheat prolamin class, given the name δ-gliadins, is the most direct ortholog of barley γ3-hordeins. Phylogenetic analysis shows that the orthologous δ-gliadins and γ3-hordeins form a distinct prolamin branch that existed separate from the γ-gliadins and γ-hordeins in an ancestral Triticeae prior to the branching of wheat and barley. The expressed δ-gliadins are encoded by a single gene in each of the hexaploid wheat genomes. This single δ-gliadin/γ3-hordein ortholog may be a general feature of the Triticeae tribe since examination of ESTs from three barley cultivars also confirms a single γ3-hordein gene. Analysis of ESTs and cDNAs shows that the genes are expressed in at least five hexaploid wheat cultivars in addition to diploids Triticum monococcum and Aegilops tauschii. The latter two sequences also allow assignment of the δ-gliadin genes to the A and D genomes, respectively, with the third sequence type assumed to be from the B genome. Two wheat cultivars for which there are sufficient ESTs show different patterns of expression, i.e., with cv Chinese Spring expressing the genes from the A and B genomes, while cv Recital has ESTs from the A and D genomes. Genomic sequences of Chinese Spring show that the D genome gene is inactivated by tandem premature stop codons. A fourth δ-gliadin sequence occurs in the D genome of both Chinese Spring and Ae. tauschii, but no ESTs match this sequence and limited genomic sequences indicates a pseudogene containing frame shifts and premature stop codons. Sequencing of BACs covering a 3 Mb region from Ae. tauschii locates the δ-gliadin gene to the complex Gli-1 plus Glu-3 region on chromosome 1.
format	article
author	Olin D Anderson Lingli Dong Naxin Huo Yong Q Gu
author_facet	Olin D Anderson Lingli Dong Naxin Huo Yong Q Gu
author_sort	Olin D Anderson
title	A new class of wheat gliadin genes and proteins.
title_short	A new class of wheat gliadin genes and proteins.
title_full	A new class of wheat gliadin genes and proteins.
title_fullStr	A new class of wheat gliadin genes and proteins.
title_full_unstemmed	A new class of wheat gliadin genes and proteins.
title_sort	new class of wheat gliadin genes and proteins.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0f147d59ab6144478bf445066321d262
work_keys_str_mv	AT olindanderson anewclassofwheatgliadingenesandproteins AT linglidong anewclassofwheatgliadingenesandproteins AT naxinhuo anewclassofwheatgliadingenesandproteins AT yongqgu anewclassofwheatgliadingenesandproteins AT olindanderson newclassofwheatgliadingenesandproteins AT linglidong newclassofwheatgliadingenesandproteins AT naxinhuo newclassofwheatgliadingenesandproteins AT yongqgu newclassofwheatgliadingenesandproteins
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A new class of wheat gliadin genes and proteins.

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