Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons

Abstract A strong focus on sex-related differences has arisen recently in neurobiology, but most investigations focus on brain function in vivo, ignoring common experimental models like cultured neurons. A few studies have addressed morphological differences between male and female neurons in cultur...

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Autores principales:	Sinem M. Sertel, Wiebke Blumenstein, Sunit Mandad, Orr Shomroni, Gabriela Salinas, Silvio O. Rizzoli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f1a0c3869b0406384c4f8fddfa03658
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spelling	oai:doaj.org-article:0f1a0c3869b0406384c4f8fddfa036582021-12-02T16:38:25ZDifferences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons10.1038/s41598-021-96846-y2045-2322https://doaj.org/article/0f1a0c3869b0406384c4f8fddfa036582021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96846-yhttps://doaj.org/toc/2045-2322Abstract A strong focus on sex-related differences has arisen recently in neurobiology, but most investigations focus on brain function in vivo, ignoring common experimental models like cultured neurons. A few studies have addressed morphological differences between male and female neurons in culture, but very few works focused on functional aspects, and especially on presynaptic function. To fill this gap, we studied here functional parameters of synaptic vesicle recycling in hippocampal cultures from male and female rats, which are a standard model system for many laboratories. We found that, although the total vesicle pools are similar, the recycling pool of male synapses was larger, and was more frequently used. This was in line with the observation that the male synapses engaged in stronger local translation. Nevertheless, the general network activity of the neurons was similar, and only small differences could be found when stimulating the cultures. We also found only limited differences in several other assays. We conclude that, albeit these cultures are similar in behavior, future studies of synapse behavior in culture should take the sex of the animals into account.Sinem M. SertelWiebke BlumensteinSunit MandadOrr ShomroniGabriela SalinasSilvio O. RizzoliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sinem M. Sertel Wiebke Blumenstein Sunit Mandad Orr Shomroni Gabriela Salinas Silvio O. Rizzoli Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
description	Abstract A strong focus on sex-related differences has arisen recently in neurobiology, but most investigations focus on brain function in vivo, ignoring common experimental models like cultured neurons. A few studies have addressed morphological differences between male and female neurons in culture, but very few works focused on functional aspects, and especially on presynaptic function. To fill this gap, we studied here functional parameters of synaptic vesicle recycling in hippocampal cultures from male and female rats, which are a standard model system for many laboratories. We found that, although the total vesicle pools are similar, the recycling pool of male synapses was larger, and was more frequently used. This was in line with the observation that the male synapses engaged in stronger local translation. Nevertheless, the general network activity of the neurons was similar, and only small differences could be found when stimulating the cultures. We also found only limited differences in several other assays. We conclude that, albeit these cultures are similar in behavior, future studies of synapse behavior in culture should take the sex of the animals into account.
format	article
author	Sinem M. Sertel Wiebke Blumenstein Sunit Mandad Orr Shomroni Gabriela Salinas Silvio O. Rizzoli
author_facet	Sinem M. Sertel Wiebke Blumenstein Sunit Mandad Orr Shomroni Gabriela Salinas Silvio O. Rizzoli
author_sort	Sinem M. Sertel
title	Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
title_short	Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
title_full	Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
title_fullStr	Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
title_full_unstemmed	Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
title_sort	differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0f1a0c3869b0406384c4f8fddfa03658
work_keys_str_mv	AT sinemmsertel differencesinsynapticvesiclepoolbehaviorbetweenmaleandfemalehippocampalculturedneurons AT wiebkeblumenstein differencesinsynapticvesiclepoolbehaviorbetweenmaleandfemalehippocampalculturedneurons AT sunitmandad differencesinsynapticvesiclepoolbehaviorbetweenmaleandfemalehippocampalculturedneurons AT orrshomroni differencesinsynapticvesiclepoolbehaviorbetweenmaleandfemalehippocampalculturedneurons AT gabrielasalinas differencesinsynapticvesiclepoolbehaviorbetweenmaleandfemalehippocampalculturedneurons AT silvioorizzoli differencesinsynapticvesiclepoolbehaviorbetweenmaleandfemalehippocampalculturedneurons
_version_	1718383557303336960

Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons

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