Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis.
<h4>Background</h4>The comparatively straightforward and cheaper light-emitting diode fluorescent microscope (LEDFM) was suggested by WHO to replace conventional microscope in tuberculosis (TB) laboratories. However, the comparable efficacy of each of those techniques differs from labora...
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oai:doaj.org-article:0f1a35e1208746838a3e6d181e38db282021-12-02T20:08:55ZDiagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis.1932-620310.1371/journal.pone.0255146https://doaj.org/article/0f1a35e1208746838a3e6d181e38db282021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255146https://doaj.org/toc/1932-6203<h4>Background</h4>The comparatively straightforward and cheaper light-emitting diode fluorescent microscope (LEDFM) was suggested by WHO to replace conventional microscope in tuberculosis (TB) laboratories. However, the comparable efficacy of each of those techniques differs from laboratory to laboratory. We investigated the efficacy of LEDFM for the diagnosis of tuberculous lymphadenitis (TBLN) patients.<h4>Methods</h4>A cross-sectional study was conducted on 211 samples from clinically suspected tuberculous lymphadenitis patients. Three smears were prepared from FNA on microscope slides for cytomorphology study, Auramine O (AO), and for Ziehl-Neelsen (ZN) staining. The left-over samples were inoculated onto Lowenstein-Jensen (LJ) media. Statistical analysis was done using STATA version 11. The sensitivity, specificity, positive and negative predictive values were calculated by considering the culture results as the gold standard using a 95% confidence interval.<h4>Results</h4>Among 211 samples 49.7% (105) were positive by cytomorphology, 32.7% (69) by LEDFM, 23.69% (50) by LJ culture, and 13.7% (29) by ZN. Compared to the gold standard sensitivity of ZN, LEDFM, and cytomorphology were 30% [95% CI: 17.9-44.6], 66% [95% CI: 51.2-78.8] 78% [95% CI: 64-88.5], respectively. The specificity of ZN, LEDFM, and cytomorphology was 91.3% [95% CI: 85.8-95.2], 77.6% [95% CI: 70.4-83.8], 58.8% [95% CI: 50.7-66.5], respectively.<h4>Conclusion</h4>LED fluorescence microscopy gives a legitimate option in contrast to conventional ZN techniques in terms of its higher sensitivity, a bit lower specificity, time-saving, and minimal effort.Gebeyehu AssefaKassu DestaShambel ArayaSelfu GirmaAdane MihretTsegaye HailuAbay AtnafuNigatu EndalaferAdugna AberaShiferaw BekeleLeila BirhanuGetu DiribaYordanos MengistuBiniyam DagneKidist BoboshaAbraham AseffaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255146 (2021) |
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Medicine R Science Q Gebeyehu Assefa Kassu Desta Shambel Araya Selfu Girma Adane Mihret Tsegaye Hailu Abay Atnafu Nigatu Endalafer Adugna Abera Shiferaw Bekele Leila Birhanu Getu Diriba Yordanos Mengistu Biniyam Dagne Kidist Bobosha Abraham Aseffa Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis. |
| description |
<h4>Background</h4>The comparatively straightforward and cheaper light-emitting diode fluorescent microscope (LEDFM) was suggested by WHO to replace conventional microscope in tuberculosis (TB) laboratories. However, the comparable efficacy of each of those techniques differs from laboratory to laboratory. We investigated the efficacy of LEDFM for the diagnosis of tuberculous lymphadenitis (TBLN) patients.<h4>Methods</h4>A cross-sectional study was conducted on 211 samples from clinically suspected tuberculous lymphadenitis patients. Three smears were prepared from FNA on microscope slides for cytomorphology study, Auramine O (AO), and for Ziehl-Neelsen (ZN) staining. The left-over samples were inoculated onto Lowenstein-Jensen (LJ) media. Statistical analysis was done using STATA version 11. The sensitivity, specificity, positive and negative predictive values were calculated by considering the culture results as the gold standard using a 95% confidence interval.<h4>Results</h4>Among 211 samples 49.7% (105) were positive by cytomorphology, 32.7% (69) by LEDFM, 23.69% (50) by LJ culture, and 13.7% (29) by ZN. Compared to the gold standard sensitivity of ZN, LEDFM, and cytomorphology were 30% [95% CI: 17.9-44.6], 66% [95% CI: 51.2-78.8] 78% [95% CI: 64-88.5], respectively. The specificity of ZN, LEDFM, and cytomorphology was 91.3% [95% CI: 85.8-95.2], 77.6% [95% CI: 70.4-83.8], 58.8% [95% CI: 50.7-66.5], respectively.<h4>Conclusion</h4>LED fluorescence microscopy gives a legitimate option in contrast to conventional ZN techniques in terms of its higher sensitivity, a bit lower specificity, time-saving, and minimal effort. |
| format |
article |
| author |
Gebeyehu Assefa Kassu Desta Shambel Araya Selfu Girma Adane Mihret Tsegaye Hailu Abay Atnafu Nigatu Endalafer Adugna Abera Shiferaw Bekele Leila Birhanu Getu Diriba Yordanos Mengistu Biniyam Dagne Kidist Bobosha Abraham Aseffa |
| author_facet |
Gebeyehu Assefa Kassu Desta Shambel Araya Selfu Girma Adane Mihret Tsegaye Hailu Abay Atnafu Nigatu Endalafer Adugna Abera Shiferaw Bekele Leila Birhanu Getu Diriba Yordanos Mengistu Biniyam Dagne Kidist Bobosha Abraham Aseffa |
| author_sort |
Gebeyehu Assefa |
| title |
Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis. |
| title_short |
Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis. |
| title_full |
Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis. |
| title_fullStr |
Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis. |
| title_full_unstemmed |
Diagnostic efficacy of Light-Emitting Diode (LED) Fluorescence based Microscope for the diagnosis of Tuberculous lymphadenitis. |
| title_sort |
diagnostic efficacy of light-emitting diode (led) fluorescence based microscope for the diagnosis of tuberculous lymphadenitis. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/0f1a35e1208746838a3e6d181e38db28 |
| work_keys_str_mv |
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