Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.

<h4>Background</h4>Preconditioning with lipopolysaccharide (LPS) is used to improve the secretion of anti-inflammatory agents in B cells. However, there are only a few studies on canine B cells.<h4>Objective</h4>This study aimed to evaluate the immune regulatory capacity of c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hee-Won Jang, Ju-Hyun An, Kyeong Bo Kim, Jeong-Hwa Lee, Ye-In Oh, Su-Min Park, Hyung-Kyu Chae, Hwa-Young Youn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f1df57961bc43d49f380324982463eb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0f1df57961bc43d49f380324982463eb
record_format	dspace
spelling	oai:doaj.org-article:0f1df57961bc43d49f380324982463eb2021-12-02T20:16:17ZCanine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.1932-620310.1371/journal.pone.0256651https://doaj.org/article/0f1df57961bc43d49f380324982463eb2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256651https://doaj.org/toc/1932-6203<h4>Background</h4>Preconditioning with lipopolysaccharide (LPS) is used to improve the secretion of anti-inflammatory agents in B cells. However, there are only a few studies on canine B cells.<h4>Objective</h4>This study aimed to evaluate the immune regulatory capacity of canine peripheral blood mononuclear cell-derived B cells pretreated with LPS.<h4>Methods</h4>Canine B cells were isolated from canine peripheral blood mononuclear cells, which were obtained from three healthy canine donors. The B cells were preconditioned with LPS, and then cell viability and the expression of the regulatory B cell marker were assessed. Finally, RNA extraction and immunofluorescence analysis were performed.<h4>Results</h4>LPS primed B cells expressed the interleukin (IL)-10 surface marker and immunoregulatory gene expression, such as IL-10, programmed death-ligand 1, and transforming growth factor beta. Macrophages in the inflammatory condition cocultured with primed B cells were found to have significantly down-regulated pro-inflammatory cytokine, such as tumor necrosis factor-α, and up-regulated anti-inflammatory cytokines such as IL-10. Additionally, it was revealed that co-culture with primed B cells re-polarized M1 macrophages to M2 macrophages.<h4>Conclusions</h4>This study revealed that LPS-primed B cells have an anti-inflammatory effect and can re-polarize macrophages, suggesting the possibility of using LPS-primed B cells as a therapeutic agent for its anti-inflammatory effects and immune modulation.Hee-Won JangJu-Hyun AnKyeong Bo KimJeong-Hwa LeeYe-In OhSu-Min ParkHyung-Kyu ChaeHwa-Young YounPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0256651 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hee-Won Jang Ju-Hyun An Kyeong Bo Kim Jeong-Hwa Lee Ye-In Oh Su-Min Park Hyung-Kyu Chae Hwa-Young Youn Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
description	<h4>Background</h4>Preconditioning with lipopolysaccharide (LPS) is used to improve the secretion of anti-inflammatory agents in B cells. However, there are only a few studies on canine B cells.<h4>Objective</h4>This study aimed to evaluate the immune regulatory capacity of canine peripheral blood mononuclear cell-derived B cells pretreated with LPS.<h4>Methods</h4>Canine B cells were isolated from canine peripheral blood mononuclear cells, which were obtained from three healthy canine donors. The B cells were preconditioned with LPS, and then cell viability and the expression of the regulatory B cell marker were assessed. Finally, RNA extraction and immunofluorescence analysis were performed.<h4>Results</h4>LPS primed B cells expressed the interleukin (IL)-10 surface marker and immunoregulatory gene expression, such as IL-10, programmed death-ligand 1, and transforming growth factor beta. Macrophages in the inflammatory condition cocultured with primed B cells were found to have significantly down-regulated pro-inflammatory cytokine, such as tumor necrosis factor-α, and up-regulated anti-inflammatory cytokines such as IL-10. Additionally, it was revealed that co-culture with primed B cells re-polarized M1 macrophages to M2 macrophages.<h4>Conclusions</h4>This study revealed that LPS-primed B cells have an anti-inflammatory effect and can re-polarize macrophages, suggesting the possibility of using LPS-primed B cells as a therapeutic agent for its anti-inflammatory effects and immune modulation.
format	article
author	Hee-Won Jang Ju-Hyun An Kyeong Bo Kim Jeong-Hwa Lee Ye-In Oh Su-Min Park Hyung-Kyu Chae Hwa-Young Youn
author_facet	Hee-Won Jang Ju-Hyun An Kyeong Bo Kim Jeong-Hwa Lee Ye-In Oh Su-Min Park Hyung-Kyu Chae Hwa-Young Youn
author_sort	Hee-Won Jang
title	Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
title_short	Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
title_full	Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
title_fullStr	Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
title_full_unstemmed	Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
title_sort	canine peripheral blood mononuclear cell-derived b lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0f1df57961bc43d49f380324982463eb
work_keys_str_mv	AT heewonjang canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT juhyunan canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT kyeongbokim canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT jeonghwalee canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT yeinoh canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT suminpark canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT hyungkyuchae canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization AT hwayoungyoun canineperipheralbloodmononuclearcellderivedblymphocytespretreatedwithlipopolysaccharideenhancetheimmunomodulatoryeffectthroughmacrophagepolarization
_version_	1718374507423465472

Canine peripheral blood mononuclear cell-derived B lymphocytes pretreated with lipopolysaccharide enhance the immunomodulatory effect through macrophage polarization.

Ejemplares similares