Novel Components of the Flagellar System in Epsilonproteobacteria

ABSTRACT Motility is essential for the pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a cell wall-anchored motor using chemical energy. Although some components of the flagellar apparatus are common to many bacteri...

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Autores principales:	Beile Gao, Maria Lara-Tejero, Matthew Lefebre, Andrew L. Goodman, Jorge E. Galán
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Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0f206e26069448bb8de32c1d149d59bb
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spelling	oai:doaj.org-article:0f206e26069448bb8de32c1d149d59bb2021-11-15T15:47:38ZNovel Components of the Flagellar System in Epsilonproteobacteria10.1128/mBio.01349-142150-7511https://doaj.org/article/0f206e26069448bb8de32c1d149d59bb2014-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01349-14https://doaj.org/toc/2150-7511ABSTRACT Motility is essential for the pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a cell wall-anchored motor using chemical energy. Although some components of the flagellar apparatus are common to many bacterial species, recent studies have shown significant differences in the flagellar structures of different bacterial species. The molecular bases for these differences, however, are not understood. The flagella from epsilonproteobacteria, which include the bacterial pathogens Campylobacter jejuni and Helicobacter pylori, are among the most divergent. Using next-generation sequencing combined with transposon mutagenesis, we have conducted a comprehensive high-throughput genetic screen in Campylobacter jejuni, which identified several novel components of its flagellar system. Biochemical analyses detected interactions between the identified proteins and known components of the flagellar machinery, and in vivo imaging located them to the bacterial poles, where flagella assemble. Most of the identified new components are conserved within but restricted to epsilonproteobacteria. These studies provide insight into the divergent flagella of this group of bacteria and highlight the complexity of this remarkable structure, which has adapted to carry out its conserved functions in the context of widely diverse bacterial species. IMPORTANCE Motility is essential for the normal physiology and pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a motor that uses chemical energy as fuel. Although some components of the flagellar apparatus are common to many bacterial species, recent studies have shown significant divergence in the flagellar structures across bacterial species. However, the molecular bases for these differences are not understood. The flagella from epsilonproteobacteria, which include the bacterial pathogens Campylobacter jejuni and Helicobacter pylori, are among the most divergent. We conducted a comprehensive genetic screen in Campylobacter jejuni and identified several novel components of the flagellar system. These studies provide important information to understand how flagella have adapted to function in the context of widely diverse sets of bacterial species and bring unique insight into the evolution and function of this remarkable bacterial organelle.Beile GaoMaria Lara-TejeroMatthew LefebreAndrew L. GoodmanJorge E. GalánAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 3 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Beile Gao Maria Lara-Tejero Matthew Lefebre Andrew L. Goodman Jorge E. Galán Novel Components of the Flagellar System in Epsilonproteobacteria
description	ABSTRACT Motility is essential for the pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a cell wall-anchored motor using chemical energy. Although some components of the flagellar apparatus are common to many bacterial species, recent studies have shown significant differences in the flagellar structures of different bacterial species. The molecular bases for these differences, however, are not understood. The flagella from epsilonproteobacteria, which include the bacterial pathogens Campylobacter jejuni and Helicobacter pylori, are among the most divergent. Using next-generation sequencing combined with transposon mutagenesis, we have conducted a comprehensive high-throughput genetic screen in Campylobacter jejuni, which identified several novel components of its flagellar system. Biochemical analyses detected interactions between the identified proteins and known components of the flagellar machinery, and in vivo imaging located them to the bacterial poles, where flagella assemble. Most of the identified new components are conserved within but restricted to epsilonproteobacteria. These studies provide insight into the divergent flagella of this group of bacteria and highlight the complexity of this remarkable structure, which has adapted to carry out its conserved functions in the context of widely diverse bacterial species. IMPORTANCE Motility is essential for the normal physiology and pathogenesis of many bacterial species. Most bacteria move using flagella, which are multiprotein filaments that rotate propelled by a motor that uses chemical energy as fuel. Although some components of the flagellar apparatus are common to many bacterial species, recent studies have shown significant divergence in the flagellar structures across bacterial species. However, the molecular bases for these differences are not understood. The flagella from epsilonproteobacteria, which include the bacterial pathogens Campylobacter jejuni and Helicobacter pylori, are among the most divergent. We conducted a comprehensive genetic screen in Campylobacter jejuni and identified several novel components of the flagellar system. These studies provide important information to understand how flagella have adapted to function in the context of widely diverse sets of bacterial species and bring unique insight into the evolution and function of this remarkable bacterial organelle.
format	article
author	Beile Gao Maria Lara-Tejero Matthew Lefebre Andrew L. Goodman Jorge E. Galán
author_facet	Beile Gao Maria Lara-Tejero Matthew Lefebre Andrew L. Goodman Jorge E. Galán
author_sort	Beile Gao
title	Novel Components of the Flagellar System in Epsilonproteobacteria
title_short	Novel Components of the Flagellar System in Epsilonproteobacteria
title_full	Novel Components of the Flagellar System in Epsilonproteobacteria
title_fullStr	Novel Components of the Flagellar System in Epsilonproteobacteria
title_full_unstemmed	Novel Components of the Flagellar System in Epsilonproteobacteria
title_sort	novel components of the flagellar system in epsilonproteobacteria
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/0f206e26069448bb8de32c1d149d59bb
work_keys_str_mv	AT beilegao novelcomponentsoftheflagellarsysteminepsilonproteobacteria AT marialaratejero novelcomponentsoftheflagellarsysteminepsilonproteobacteria AT matthewlefebre novelcomponentsoftheflagellarsysteminepsilonproteobacteria AT andrewlgoodman novelcomponentsoftheflagellarsysteminepsilonproteobacteria AT jorgeegalan novelcomponentsoftheflagellarsysteminepsilonproteobacteria
_version_	1718427501743570944

Novel Components of the Flagellar System in Epsilonproteobacteria

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