An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to...

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Autores principales:	Jadranka Milosevic, Mengrou Lu, Wallace Greene, Hong-Zhang He, Si-Yang Zheng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	ultrafast one-step RT-qPCR assay SARS-CoV-2 detection COVID-19 nasopharyngeal swab Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0f23aaf52ea64b4ba3ecd0d4c47ef909
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spelling	oai:doaj.org-article:0f23aaf52ea64b4ba3ecd0d4c47ef9092021-11-04T08:24:07ZAn Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-21664-302X10.3389/fmicb.2021.749783https://doaj.org/article/0f23aaf52ea64b4ba3ecd0d4c47ef9092021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.749783/fullhttps://doaj.org/toc/1664-302XWe developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.Jadranka MilosevicJadranka MilosevicMengrou LuWallace GreeneHong-Zhang HeHong-Zhang HeSi-Yang ZhengSi-Yang ZhengFrontiers Media S.A.articleultrafastone-step RT-qPCR assaySARS-CoV-2 detectionCOVID-19nasopharyngeal swabMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ultrafast one-step RT-qPCR assay SARS-CoV-2 detection COVID-19 nasopharyngeal swab Microbiology QR1-502
spellingShingle	ultrafast one-step RT-qPCR assay SARS-CoV-2 detection COVID-19 nasopharyngeal swab Microbiology QR1-502 Jadranka Milosevic Jadranka Milosevic Mengrou Lu Wallace Greene Hong-Zhang He Hong-Zhang He Si-Yang Zheng Si-Yang Zheng An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
description	We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.
format	article
author	Jadranka Milosevic Jadranka Milosevic Mengrou Lu Wallace Greene Hong-Zhang He Hong-Zhang He Si-Yang Zheng Si-Yang Zheng
author_facet	Jadranka Milosevic Jadranka Milosevic Mengrou Lu Wallace Greene Hong-Zhang He Hong-Zhang He Si-Yang Zheng Si-Yang Zheng
author_sort	Jadranka Milosevic
title	An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_short	An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_full	An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_fullStr	An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_full_unstemmed	An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
title_sort	ultrafast one-step quantitative reverse transcription–polymerase chain reaction assay for detection of sars-cov-2
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0f23aaf52ea64b4ba3ecd0d4c47ef909
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An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

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