Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.

To identify long non-coding RNAs (lncRNAs) and their potential roles in hepatic fibrosis in rat liver issues induced by CCl4, lncRNAs and genes were analyzed in fibrotic rat liver tissues by RNA sequencing and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Differ...

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Autores principales:	Yan Wang, Xiong Xiao, Xiaobo Wang, Feng Guo, Xiaozhong Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f3a22c7d56c4bfa84db943a908e6c5d
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spelling	oai:doaj.org-article:0f3a22c7d56c4bfa84db943a908e6c5d2021-12-02T20:17:24ZIdentification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.1932-620310.1371/journal.pone.0258194https://doaj.org/article/0f3a22c7d56c4bfa84db943a908e6c5d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258194https://doaj.org/toc/1932-6203To identify long non-coding RNAs (lncRNAs) and their potential roles in hepatic fibrosis in rat liver issues induced by CCl4, lncRNAs and genes were analyzed in fibrotic rat liver tissues by RNA sequencing and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Differentially expressed (DE) lncRNAs (DE-lncRNAs) and genes were subjected to bioinformatics analysis and used to construct a co-expression network. We identified 10 novel DE-lncRNAs that were downregulated during the hepatic fibrosis process. The cis target gene of DE-lncRNA, XLOC118358, was Met, and the cis target gene of the other nine DE-lncRNAs, XLOC004600, XLOC004605, XLOC004610, XLOC004611, XLOC004568, XLOC004580 XLOC004598, XLOC004601, and XLOC004602 was Nox4. The results of construction of a pathway-DEG co-expression network show that lncRNA-Met and lncRNAs-Nox4 were involved in oxidation-reduction processes and PI3K/Akt signaling pathway. Our results identified 10 DE-lncRNAs related to hepatic fibrosis, and the potential roles of DE-lncRNAs and target genes in hepatic fibrosis might provide new therapeutic strategies for hepatic fibrosis.Yan WangXiong XiaoXiaobo WangFeng GuoXiaozhong WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258194 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Wang Xiong Xiao Xiaobo Wang Feng Guo Xiaozhong Wang Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.
description	To identify long non-coding RNAs (lncRNAs) and their potential roles in hepatic fibrosis in rat liver issues induced by CCl4, lncRNAs and genes were analyzed in fibrotic rat liver tissues by RNA sequencing and verified by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Differentially expressed (DE) lncRNAs (DE-lncRNAs) and genes were subjected to bioinformatics analysis and used to construct a co-expression network. We identified 10 novel DE-lncRNAs that were downregulated during the hepatic fibrosis process. The cis target gene of DE-lncRNA, XLOC118358, was Met, and the cis target gene of the other nine DE-lncRNAs, XLOC004600, XLOC004605, XLOC004610, XLOC004611, XLOC004568, XLOC004580 XLOC004598, XLOC004601, and XLOC004602 was Nox4. The results of construction of a pathway-DEG co-expression network show that lncRNA-Met and lncRNAs-Nox4 were involved in oxidation-reduction processes and PI3K/Akt signaling pathway. Our results identified 10 DE-lncRNAs related to hepatic fibrosis, and the potential roles of DE-lncRNAs and target genes in hepatic fibrosis might provide new therapeutic strategies for hepatic fibrosis.
format	article
author	Yan Wang Xiong Xiao Xiaobo Wang Feng Guo Xiaozhong Wang
author_facet	Yan Wang Xiong Xiao Xiaobo Wang Feng Guo Xiaozhong Wang
author_sort	Yan Wang
title	Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.
title_short	Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.
title_full	Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.
title_fullStr	Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.
title_full_unstemmed	Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.
title_sort	identification of differentially expressed long noncoding rnas and pathways in liver tissues from rats with hepatic fibrosis.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/0f3a22c7d56c4bfa84db943a908e6c5d
work_keys_str_mv	AT yanwang identificationofdifferentiallyexpressedlongnoncodingrnasandpathwaysinlivertissuesfromratswithhepaticfibrosis AT xiongxiao identificationofdifferentiallyexpressedlongnoncodingrnasandpathwaysinlivertissuesfromratswithhepaticfibrosis AT xiaobowang identificationofdifferentiallyexpressedlongnoncodingrnasandpathwaysinlivertissuesfromratswithhepaticfibrosis AT fengguo identificationofdifferentiallyexpressedlongnoncodingrnasandpathwaysinlivertissuesfromratswithhepaticfibrosis AT xiaozhongwang identificationofdifferentiallyexpressedlongnoncodingrnasandpathwaysinlivertissuesfromratswithhepaticfibrosis
_version_	1718374390378266624

Identification of differentially expressed long noncoding RNAs and pathways in liver tissues from rats with hepatic fibrosis.

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