Microbiological diagnostics of pyoinflammatory abdominal diseases

The aim of the work was to establish the significance of complex bacteriological research in the diagnostics of acute appendicitis and to determine an optimal material for the research based on the findings. We examined 19 patients with acute phlegmonous or acute gangrenous appendicitis (males, aged...

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Autores principales:	T. V. Fadeeva, I. A. Shurygina, M. G. Shurygin, A. V. Vetokhina, L. M. Vladimirova, N. I. Ayushinova, A. I. Panasyuk
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	acute appendicitis diagnostics microscopic flora exudate biopsy specimen antibiotic resistance Science Q
Acceso en línea:	https://doaj.org/article/0f3cac1475064b0680cb55d5b67ed42e
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id	oai:doaj.org-article:0f3cac1475064b0680cb55d5b67ed42e
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spelling	oai:doaj.org-article:0f3cac1475064b0680cb55d5b67ed42e2021-11-23T06:14:35ZMicrobiological diagnostics of pyoinflammatory abdominal diseases2541-94202587-959610.12737/22976https://doaj.org/article/0f3cac1475064b0680cb55d5b67ed42e2016-07-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/249https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The aim of the work was to establish the significance of complex bacteriological research in the diagnostics of acute appendicitis and to determine an optimal material for the research based on the findings. We examined 19 patients with acute phlegmonous or acute gangrenous appendicitis (males, aged 18-60 years). We performed bacteriological research of abdominal exudate (n = 19) and biopsy specimen (n = l9) of appendix wall taken before opening the lumen of the intestine. Both abdominal exudate and appendix wall specimen were taken at the same time. Aerobic and anaerobic microorganisms were detected and identified, antimicrobial susceptibility was tested. In total, we detected 25 strains of aerobic and 13 strains of anaerobic microorganisms. It has been established that a bioptate was most informative for testing (68.4 %); the parallel study of an abdominal exudate gave positive results in 21.1 % of cases. In the structure of clinically significant microflora dominated E. coli (43.3 %), then went nonfermentative gram-negative bacteria (13.3 %) and Bacteroides spp. (16.7 %). We marked growing resistance of detected strains of gram-negative bacteria to some antibiotics. For instance, 62 % of detected E. coli strains were resistant to ampicillin, 25 % - to ciprofloxacin. 92 % of strains were resistant to cefepime, 93 % -to ceftriaxone, 77 % - to Amoxiclav, 67 % - to gentamicin, 90 % - to tobramycin. From one bioptate a strain of E. coli ESBL was separated. The study of intraoperative bioptate of appendix wall increases effectiveness of microbiological diagnostics in comparison with the abdominal exudate research.T. V. FadeevaI. A. ShuryginaM. G. ShuryginA. V. VetokhinaL. M. VladimirovaN. I. AyushinovaA. I. PanasyukScientific Сentre for Family Health and Human Reproduction Problemsarticleacute appendicitisdiagnosticsmicroscopic floraexudatebiopsy specimenantibiotic resistanceScienceQRUActa Biomedica Scientifica, Vol 1, Iss 4, Pp 95-98 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	acute appendicitis diagnostics microscopic flora exudate biopsy specimen antibiotic resistance Science Q
spellingShingle	acute appendicitis diagnostics microscopic flora exudate biopsy specimen antibiotic resistance Science Q T. V. Fadeeva I. A. Shurygina M. G. Shurygin A. V. Vetokhina L. M. Vladimirova N. I. Ayushinova A. I. Panasyuk Microbiological diagnostics of pyoinflammatory abdominal diseases
description	The aim of the work was to establish the significance of complex bacteriological research in the diagnostics of acute appendicitis and to determine an optimal material for the research based on the findings. We examined 19 patients with acute phlegmonous or acute gangrenous appendicitis (males, aged 18-60 years). We performed bacteriological research of abdominal exudate (n = 19) and biopsy specimen (n = l9) of appendix wall taken before opening the lumen of the intestine. Both abdominal exudate and appendix wall specimen were taken at the same time. Aerobic and anaerobic microorganisms were detected and identified, antimicrobial susceptibility was tested. In total, we detected 25 strains of aerobic and 13 strains of anaerobic microorganisms. It has been established that a bioptate was most informative for testing (68.4 %); the parallel study of an abdominal exudate gave positive results in 21.1 % of cases. In the structure of clinically significant microflora dominated E. coli (43.3 %), then went nonfermentative gram-negative bacteria (13.3 %) and Bacteroides spp. (16.7 %). We marked growing resistance of detected strains of gram-negative bacteria to some antibiotics. For instance, 62 % of detected E. coli strains were resistant to ampicillin, 25 % - to ciprofloxacin. 92 % of strains were resistant to cefepime, 93 % -to ceftriaxone, 77 % - to Amoxiclav, 67 % - to gentamicin, 90 % - to tobramycin. From one bioptate a strain of E. coli ESBL was separated. The study of intraoperative bioptate of appendix wall increases effectiveness of microbiological diagnostics in comparison with the abdominal exudate research.
format	article
author	T. V. Fadeeva I. A. Shurygina M. G. Shurygin A. V. Vetokhina L. M. Vladimirova N. I. Ayushinova A. I. Panasyuk
author_facet	T. V. Fadeeva I. A. Shurygina M. G. Shurygin A. V. Vetokhina L. M. Vladimirova N. I. Ayushinova A. I. Panasyuk
author_sort	T. V. Fadeeva
title	Microbiological diagnostics of pyoinflammatory abdominal diseases
title_short	Microbiological diagnostics of pyoinflammatory abdominal diseases
title_full	Microbiological diagnostics of pyoinflammatory abdominal diseases
title_fullStr	Microbiological diagnostics of pyoinflammatory abdominal diseases
title_full_unstemmed	Microbiological diagnostics of pyoinflammatory abdominal diseases
title_sort	microbiological diagnostics of pyoinflammatory abdominal diseases
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/0f3cac1475064b0680cb55d5b67ed42e
work_keys_str_mv	AT tvfadeeva microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases AT iashurygina microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases AT mgshurygin microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases AT avvetokhina microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases AT lmvladimirova microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases AT niayushinova microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases AT aipanasyuk microbiologicaldiagnosticsofpyoinflammatoryabdominaldiseases
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Microbiological diagnostics of pyoinflammatory abdominal diseases

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