Laboratory assessment of oxidative stress in semen

Objectives: To evaluate different laboratory assessments of oxidative stress (OS) in semen and identify a cost-efficient and highly sensitive instrument capable of providing a comprehensive measure of OS in a clinical setting, as early intervention and an accurate diagnostic test are important becau...

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Autores principales:	Ashok Agarwal, Emily Qiu, Rakesh Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/0f453fb86bd94375945c1b88ff194bee
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spelling	oai:doaj.org-article:0f453fb86bd94375945c1b88ff194bee2021-12-02T12:02:03ZLaboratory assessment of oxidative stress in semen2090-598X10.1016/j.aju.2017.11.008https://doaj.org/article/0f453fb86bd94375945c1b88ff194bee2018-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2090598X17301420https://doaj.org/toc/2090-598XObjectives: To evaluate different laboratory assessments of oxidative stress (OS) in semen and identify a cost-efficient and highly sensitive instrument capable of providing a comprehensive measure of OS in a clinical setting, as early intervention and an accurate diagnostic test are important because they help maintain a balance of free radicals and antioxidants; otherwise, excessive OS could lead to sperm damage and result in male infertility. Materials and methods: A systematic literature search was performed through a MedLine database search using the keywords ‘semen’ AND ‘oxygen reduction potential’. We also reviewed the references of retrieved articles to search for other potentially relevant research articles and additional book chapters discussing laboratory assessments for OS, ranging from 1994 to 2017. A total of 29 articles and book chapters involving OS-related laboratory assays were included. We excluded animal studies and articles written in languages other than English. Results: Direct laboratory techniques include: chemiluminescence, nitro blue tetrazolium, cytochrome C reduction test, fluorescein probe, electron spin resonance and oxidation–reduction potential (ORP). Indirect laboratory techniques include: measurement of Endtz test, lipid peroxidation, chemokines, antioxidants/micronutrients/vitamins, ascorbate, total antioxidant capacity, or DNA damage. Each of these laboratory techniques has its advantages and disadvantages. Conclusion: Traditional OS laboratory assessments have their limitations. Amongst the prevalent laboratory techniques, ORP is novel and better option as it can be easily used in a clinical setting to provide a comprehensive review of OS. However, more studies are needed to evaluate its reproducibility across various laboratory centres. Keywords: Semen, male infertility, Oxidative stress, Chemiluminescence, Total antioxidant capacity, Oxidation-reduction potentialAshok AgarwalEmily QiuRakesh SharmaTaylor & Francis GrouparticleDiseases of the genitourinary system. UrologyRC870-923ENArab Journal of Urology, Vol 16, Iss 1, Pp 77-86 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diseases of the genitourinary system. Urology RC870-923
spellingShingle	Diseases of the genitourinary system. Urology RC870-923 Ashok Agarwal Emily Qiu Rakesh Sharma Laboratory assessment of oxidative stress in semen
description	Objectives: To evaluate different laboratory assessments of oxidative stress (OS) in semen and identify a cost-efficient and highly sensitive instrument capable of providing a comprehensive measure of OS in a clinical setting, as early intervention and an accurate diagnostic test are important because they help maintain a balance of free radicals and antioxidants; otherwise, excessive OS could lead to sperm damage and result in male infertility. Materials and methods: A systematic literature search was performed through a MedLine database search using the keywords ‘semen’ AND ‘oxygen reduction potential’. We also reviewed the references of retrieved articles to search for other potentially relevant research articles and additional book chapters discussing laboratory assessments for OS, ranging from 1994 to 2017. A total of 29 articles and book chapters involving OS-related laboratory assays were included. We excluded animal studies and articles written in languages other than English. Results: Direct laboratory techniques include: chemiluminescence, nitro blue tetrazolium, cytochrome C reduction test, fluorescein probe, electron spin resonance and oxidation–reduction potential (ORP). Indirect laboratory techniques include: measurement of Endtz test, lipid peroxidation, chemokines, antioxidants/micronutrients/vitamins, ascorbate, total antioxidant capacity, or DNA damage. Each of these laboratory techniques has its advantages and disadvantages. Conclusion: Traditional OS laboratory assessments have their limitations. Amongst the prevalent laboratory techniques, ORP is novel and better option as it can be easily used in a clinical setting to provide a comprehensive review of OS. However, more studies are needed to evaluate its reproducibility across various laboratory centres. Keywords: Semen, male infertility, Oxidative stress, Chemiluminescence, Total antioxidant capacity, Oxidation-reduction potential
format	article
author	Ashok Agarwal Emily Qiu Rakesh Sharma
author_facet	Ashok Agarwal Emily Qiu Rakesh Sharma
author_sort	Ashok Agarwal
title	Laboratory assessment of oxidative stress in semen
title_short	Laboratory assessment of oxidative stress in semen
title_full	Laboratory assessment of oxidative stress in semen
title_fullStr	Laboratory assessment of oxidative stress in semen
title_full_unstemmed	Laboratory assessment of oxidative stress in semen
title_sort	laboratory assessment of oxidative stress in semen
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/0f453fb86bd94375945c1b88ff194bee
work_keys_str_mv	AT ashokagarwal laboratoryassessmentofoxidativestressinsemen AT emilyqiu laboratoryassessmentofoxidativestressinsemen AT rakeshsharma laboratoryassessmentofoxidativestressinsemen
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Laboratory assessment of oxidative stress in semen

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