Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes

Abstract Calcium-dependent protein kinases (CDPKs) are crucial calcium sensors that play important roles in the regulation of plant growth and developmental processes, as well as protective responses to environmental stress. Here, we identified 28 CDPK genes from barley and cloned 5 new, full-length...

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Autores principales:	Yunqiang Yang, Qiuli Wang, Qian Chen, Xin Yin, Min Qian, Xudong Sun, Yongping Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f45aa79307d4355a915ae4516e779da
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spelling	oai:doaj.org-article:0f45aa79307d4355a915ae4516e779da2021-12-02T15:04:51ZGenome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes10.1038/s41598-017-05646-w2045-2322https://doaj.org/article/0f45aa79307d4355a915ae4516e779da2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05646-whttps://doaj.org/toc/2045-2322Abstract Calcium-dependent protein kinases (CDPKs) are crucial calcium sensors that play important roles in the regulation of plant growth and developmental processes, as well as protective responses to environmental stress. Here, we identified 28 CDPK genes from barley and cloned 5 new, full-length CDPK genes, MLOC_58648a, MLOC_19618a, MLOC_71733a, AK249361a and MLOC_4965a, using their expressed sequence tags. Phylogenetic and gene structural analyses revealed that the CDPK could be divided into four subgroups. Significant site-specific altered constraints and a high evolutionary rate may have contributed to the functional divergences among CDPK gene subfamilies. Expression profiles of different tissues and developmental stages suggested that several CDPK genes are involved in the functional development of plants. Different expression levels under a variety of abiotic stresses also indicated that the CDPK family underwent functional divergence during long-term evolution. Furthermore, several CDPK genes responded to single treatments and individual CDPK genes responded to multiple treatments, suggesting that barley CDPKs may be involved in mediating cross-talk among different signalling pathways. Our data provide an important foundation for the functional and evolutionary analyses of this important gene family in barley.Yunqiang YangQiuli WangQian ChenXin YinMin QianXudong SunYongping YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yunqiang Yang Qiuli Wang Qian Chen Xin Yin Min Qian Xudong Sun Yongping Yang Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes
description	Abstract Calcium-dependent protein kinases (CDPKs) are crucial calcium sensors that play important roles in the regulation of plant growth and developmental processes, as well as protective responses to environmental stress. Here, we identified 28 CDPK genes from barley and cloned 5 new, full-length CDPK genes, MLOC_58648a, MLOC_19618a, MLOC_71733a, AK249361a and MLOC_4965a, using their expressed sequence tags. Phylogenetic and gene structural analyses revealed that the CDPK could be divided into four subgroups. Significant site-specific altered constraints and a high evolutionary rate may have contributed to the functional divergences among CDPK gene subfamilies. Expression profiles of different tissues and developmental stages suggested that several CDPK genes are involved in the functional development of plants. Different expression levels under a variety of abiotic stresses also indicated that the CDPK family underwent functional divergence during long-term evolution. Furthermore, several CDPK genes responded to single treatments and individual CDPK genes responded to multiple treatments, suggesting that barley CDPKs may be involved in mediating cross-talk among different signalling pathways. Our data provide an important foundation for the functional and evolutionary analyses of this important gene family in barley.
format	article
author	Yunqiang Yang Qiuli Wang Qian Chen Xin Yin Min Qian Xudong Sun Yongping Yang
author_facet	Yunqiang Yang Qiuli Wang Qian Chen Xin Yin Min Qian Xudong Sun Yongping Yang
author_sort	Yunqiang Yang
title	Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes
title_short	Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes
title_full	Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes
title_fullStr	Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes
title_full_unstemmed	Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes
title_sort	genome-wide survey indicates diverse physiological roles of the barley (hordeum vulgare l.) calcium-dependent protein kinase genes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0f45aa79307d4355a915ae4516e779da
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Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes

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