Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa

Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa

Consistent intake of legumes has been correlated with decreased possibility of developing colorectal cancer (CRC) due to the content of some phytochemicals like polyphenols. Bambara groundnut (BGN) is an underutilized crop with a rich nutritional profile, but have not been exploited for its nutraceu...

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Autores principales: Jane N.C. Okafor, Fanie Rautenbauch, Mervin Meyer, Marilize Le Roes-Hill, Taahir Harris, Victoria A. Jideani
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
Materias:
Bambara groundnut
Cotyledon
Seed coat
Phenolic
Antioxidant activity
Colon cancer
Science (General)
Q1-390
Social sciences (General)
H1-99
Acceso en línea:https://doaj.org/article/0f484760af40430abb5d796bfc5014a6
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spelling oai:doaj.org-article:0f484760af40430abb5d796bfc5014a62021-12-02T05:03:01ZPhenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa2405-844010.1016/j.heliyon.2021.e08397https://doaj.org/article/0f484760af40430abb5d796bfc5014a62021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021025007https://doaj.org/toc/2405-8440Consistent intake of legumes has been correlated with decreased possibility of developing colorectal cancer (CRC) due to the content of some phytochemicals like polyphenols. Bambara groundnut (BGN) is an underutilized crop with a rich nutritional profile, but have not been exploited for its nutraceutical and medicinal benefits. In this study, total polyphenol, flavonoid (flavonol and flavanol) content, antioxidant activity and cytotoxicity/antiproliferative properties of 70% ethanolic extracts of whole BGN, cotyledon and seed coat on Caco-2 and HT-29 colon cancer cells were evaluated. Seed coat had a significantly (p < 0.05) higher composition of total polyphenol, flavonol and flavan-3-ol (flavanol) compared to whole seed and cotyledon. Antioxidant activity determined with ferric reducing antioxidant power (FRAP), 2,2- azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assays, showed that seed coat with higher polyphenolic content had significantly (p < 0.05) greater antioxidant activity. BGN fractions demonstrated cytotoxic and antiproliferative effects against HT-29 and Caco-2 colon cancer cells in a dose-dependent manner, with seed coat and whole seed exhibiting greater cytotoxicity and higher antiproliferative activity and colon cancer cell inhibition. Extracts of the cotyledon also showed cytotoxic activity and hindered cancer cell growth/division but to a significantly (p < 0.05) lower magnitude. BGN parts indicated a greater cytotoxic effect and potential to slow down Caco-2 colon cancer cell growth and division over HT-29. This result provides new knowledge on the possible health benefits of BGN, as well as the potential for product development and may influence its consumption and utilisation.Jane N.C. OkaforFanie RautenbauchMervin MeyerMarilize Le Roes-HillTaahir HarrisVictoria A. JideaniElsevierarticleBambara groundnutCotyledonSeed coatPhenolicAntioxidant activityColon cancerScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08397- (2021)
institution DOAJ
collection DOAJ
language EN
topic Bambara groundnut
Cotyledon
Seed coat
Phenolic
Antioxidant activity
Colon cancer
Science (General)
Q1-390
Social sciences (General)
H1-99
spellingShingle Bambara groundnut
Cotyledon
Seed coat
Phenolic
Antioxidant activity
Colon cancer
Science (General)
Q1-390
Social sciences (General)
H1-99
Jane N.C. Okafor
Fanie Rautenbauch
Mervin Meyer
Marilize Le Roes-Hill
Taahir Harris
Victoria A. Jideani
Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa
description Consistent intake of legumes has been correlated with decreased possibility of developing colorectal cancer (CRC) due to the content of some phytochemicals like polyphenols. Bambara groundnut (BGN) is an underutilized crop with a rich nutritional profile, but have not been exploited for its nutraceutical and medicinal benefits. In this study, total polyphenol, flavonoid (flavonol and flavanol) content, antioxidant activity and cytotoxicity/antiproliferative properties of 70% ethanolic extracts of whole BGN, cotyledon and seed coat on Caco-2 and HT-29 colon cancer cells were evaluated. Seed coat had a significantly (p < 0.05) higher composition of total polyphenol, flavonol and flavan-3-ol (flavanol) compared to whole seed and cotyledon. Antioxidant activity determined with ferric reducing antioxidant power (FRAP), 2,2- azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) assays, showed that seed coat with higher polyphenolic content had significantly (p < 0.05) greater antioxidant activity. BGN fractions demonstrated cytotoxic and antiproliferative effects against HT-29 and Caco-2 colon cancer cells in a dose-dependent manner, with seed coat and whole seed exhibiting greater cytotoxicity and higher antiproliferative activity and colon cancer cell inhibition. Extracts of the cotyledon also showed cytotoxic activity and hindered cancer cell growth/division but to a significantly (p < 0.05) lower magnitude. BGN parts indicated a greater cytotoxic effect and potential to slow down Caco-2 colon cancer cell growth and division over HT-29. This result provides new knowledge on the possible health benefits of BGN, as well as the potential for product development and may influence its consumption and utilisation.
format article
author Jane N.C. Okafor
Fanie Rautenbauch
Mervin Meyer
Marilize Le Roes-Hill
Taahir Harris
Victoria A. Jideani
author_facet Jane N.C. Okafor
Fanie Rautenbauch
Mervin Meyer
Marilize Le Roes-Hill
Taahir Harris
Victoria A. Jideani
author_sort Jane N.C. Okafor
title Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa
title_short Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa
title_full Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa
title_fullStr Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa
title_full_unstemmed Phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of Vigna subterraenea (L.) verdc from Mpumalanga, South Africa
title_sort phenolic content, antioxidant, cytotoxic and antiproliferative effects of fractions of vigna subterraenea (l.) verdc from mpumalanga, south africa
publisher Elsevier
publishDate 2021
url https://doaj.org/article/0f484760af40430abb5d796bfc5014a6
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