Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice

In this study, we aimed to observe the effects of Loofah cylindrica (LC) extract on learning and memory ability, brain tissue morphology, and immune function of aging mice. Kunming mice were selected and randomly divided into a control group, a positive control group, an aging group, and three dose...

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Autores principales:	Dong Limin, Liu Chunjie
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	loofah cylindrica extract aging d-galactose learning and memory ability morphology immune regulation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0f4cbf4d224e42dab90238c3e3402611
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spelling	oai:doaj.org-article:0f4cbf4d224e42dab90238c3e34026112021-12-05T14:10:41ZEffects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice2391-541210.1515/biol-2021-0046https://doaj.org/article/0f4cbf4d224e42dab90238c3e34026112021-04-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0046https://doaj.org/toc/2391-5412In this study, we aimed to observe the effects of Loofah cylindrica (LC) extract on learning and memory ability, brain tissue morphology, and immune function of aging mice. Kunming mice were selected and randomly divided into a control group, a positive control group, an aging group, and three dose groups. Three dose groups were administered 187.5, 375, and 750 mg/kg of LC extract, respectively. Except for the control group, the mice in any other group were continuously subcutaneously injected d-galactose on the back and neck. Platform tests and Morris water maze (MWM) were adopted to test the learning and memory ability of each group. The brain index, thymus index, and spleen index of each group were determined by the organ-to-body ratio method. The enzyme-linked immunosorbent assay was performed to measure the concentration of cytokines interleukin 2 and interferon gamma and the proliferation activity of T lymphocytes in mouse serum. In addition, the hematoxylin and eosin staining was employed to observe the morphological changes in mouse brain tissues of each group. The results show that the aging group made more errors in the platform test, had longer escape latency, shorter swimming time in the platform quadrant, and fewer platform crossings in the MWM; much fewer brain tissue cells; and smaller brain index, thymus index, and spleen index. The LC extracts (375 and 750 mg/kg) can significantly antagonize the changes in the above indices. It can be concluded that LC extract can improve the learning and memory of aging mice, enhance their immune activity, and delay the aging process.Dong LiminLiu ChunjieDe Gruyterarticleloofah cylindrica extractagingd-galactoselearning and memory abilitymorphologyimmune regulationBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 399-407 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	loofah cylindrica extract aging d-galactose learning and memory ability morphology immune regulation Biology (General) QH301-705.5
spellingShingle	loofah cylindrica extract aging d-galactose learning and memory ability morphology immune regulation Biology (General) QH301-705.5 Dong Limin Liu Chunjie Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
description	In this study, we aimed to observe the effects of Loofah cylindrica (LC) extract on learning and memory ability, brain tissue morphology, and immune function of aging mice. Kunming mice were selected and randomly divided into a control group, a positive control group, an aging group, and three dose groups. Three dose groups were administered 187.5, 375, and 750 mg/kg of LC extract, respectively. Except for the control group, the mice in any other group were continuously subcutaneously injected d-galactose on the back and neck. Platform tests and Morris water maze (MWM) were adopted to test the learning and memory ability of each group. The brain index, thymus index, and spleen index of each group were determined by the organ-to-body ratio method. The enzyme-linked immunosorbent assay was performed to measure the concentration of cytokines interleukin 2 and interferon gamma and the proliferation activity of T lymphocytes in mouse serum. In addition, the hematoxylin and eosin staining was employed to observe the morphological changes in mouse brain tissues of each group. The results show that the aging group made more errors in the platform test, had longer escape latency, shorter swimming time in the platform quadrant, and fewer platform crossings in the MWM; much fewer brain tissue cells; and smaller brain index, thymus index, and spleen index. The LC extracts (375 and 750 mg/kg) can significantly antagonize the changes in the above indices. It can be concluded that LC extract can improve the learning and memory of aging mice, enhance their immune activity, and delay the aging process.
format	article
author	Dong Limin Liu Chunjie
author_facet	Dong Limin Liu Chunjie
author_sort	Dong Limin
title	Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
title_short	Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
title_full	Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
title_fullStr	Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
title_full_unstemmed	Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
title_sort	effects of loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/0f4cbf4d224e42dab90238c3e3402611
work_keys_str_mv	AT donglimin effectsofloofahcylindricaextractonlearningandmemoryabilitybraintissuemorphologyandimmunefunctionofagingmice AT liuchunjie effectsofloofahcylindricaextractonlearningandmemoryabilitybraintissuemorphologyandimmunefunctionofagingmice
_version_	1718371822779498496

Effects of Loofah cylindrica extract on learning and memory ability, brain tissue morphology, and immune function of aging mice

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