Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt

A total of five Escherichia coli and eight Salmonella isolates were recovered from ready-to-eat meat samples obtained from different street vendors in Luxor city, Egypt. Bacterial isolates were assayed for antimicrobial susceptibility, its virulence and antimicrobial resistance genes. The total numb...

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Autores principales:	Rania I. Younis, Soad A. Nasef, Wesam M. Salem
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2019
Materias:	Escherichia coli Meat products Salmonella spp virulence genes Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/0f4f12b48a9e4a7d9f2aba9037698c2a
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spelling	oai:doaj.org-article:0f4f12b48a9e4a7d9f2aba9037698c2a2021-12-02T04:01:03ZDetection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt10.21608/svu.2019.231682535-18262535-1877https://doaj.org/article/0f4f12b48a9e4a7d9f2aba9037698c2a2019-01-01T00:00:00Zhttps://svu.journals.ekb.eg/article_23168.htmlhttps://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877A total of five Escherichia coli and eight Salmonella isolates were recovered from ready-to-eat meat samples obtained from different street vendors in Luxor city, Egypt. Bacterial isolates were assayed for antimicrobial susceptibility, its virulence and antimicrobial resistance genes. The total number recovered positive Salmonella spp and E. coli were 8 (6.66%) and 5 (4.16%) respectively. All E. coli isolates were exhibited resistance against streptomycin and cephalothin. While all Salmonella isolates were resistant to nalidixic acid. PCR screening for virulence genes showed that 2 (40%) of the E. coli (O111:H4) serovar were positive for stx1, stx2, and eaeA. While Salmonella enteritidis, typhimurium, and virchow hold invA, hilA and stn genes with percentage of 37.5, 25 and 12.5% respectively. The identified tetracycline resistance gene for E. coli isolates were tetB (60%), tetC (20%) and tetD (20%). The β-lactamase resistance gene blaCTX was identified in 50% of Salmonella isolates represented by S. enteritidis, S. typhimurium and S. Virchow. The blaCMY genes were detected in S. typhimurium and S. infantis (37.5%). These results highlighted the role of ready-to-eat meat as a potential source for multidrug-resistant strains of E. coli and Salmonella. The current results indicate the need for applying hygienic practices in food outlets - especially in street vendors - to reduce the incidence of food-borne bacteria and to prevent future food-borne outbreaks in the studied area.Rania I. Younis Soad A. NasefWesam M. Salem South Valley UniversityarticleEscherichia coliMeat productsSalmonella sppvirulence genesAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 2, Iss 1, Pp 20-35 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Escherichia coli Meat products Salmonella spp virulence genes Agriculture S Veterinary medicine SF600-1100
spellingShingle	Escherichia coli Meat products Salmonella spp virulence genes Agriculture S Veterinary medicine SF600-1100 Rania I. Younis Soad A. Nasef Wesam M. Salem Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt
description	A total of five Escherichia coli and eight Salmonella isolates were recovered from ready-to-eat meat samples obtained from different street vendors in Luxor city, Egypt. Bacterial isolates were assayed for antimicrobial susceptibility, its virulence and antimicrobial resistance genes. The total number recovered positive Salmonella spp and E. coli were 8 (6.66%) and 5 (4.16%) respectively. All E. coli isolates were exhibited resistance against streptomycin and cephalothin. While all Salmonella isolates were resistant to nalidixic acid. PCR screening for virulence genes showed that 2 (40%) of the E. coli (O111:H4) serovar were positive for stx1, stx2, and eaeA. While Salmonella enteritidis, typhimurium, and virchow hold invA, hilA and stn genes with percentage of 37.5, 25 and 12.5% respectively. The identified tetracycline resistance gene for E. coli isolates were tetB (60%), tetC (20%) and tetD (20%). The β-lactamase resistance gene blaCTX was identified in 50% of Salmonella isolates represented by S. enteritidis, S. typhimurium and S. Virchow. The blaCMY genes were detected in S. typhimurium and S. infantis (37.5%). These results highlighted the role of ready-to-eat meat as a potential source for multidrug-resistant strains of E. coli and Salmonella. The current results indicate the need for applying hygienic practices in food outlets - especially in street vendors - to reduce the incidence of food-borne bacteria and to prevent future food-borne outbreaks in the studied area.
format	article
author	Rania I. Younis Soad A. Nasef Wesam M. Salem
author_facet	Rania I. Younis Soad A. Nasef Wesam M. Salem
author_sort	Rania I. Younis
title	Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt
title_short	Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt
title_full	Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt
title_fullStr	Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt
title_full_unstemmed	Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt
title_sort	detection of multi-drug resistant food-borne bacteria in ready-to-eat meat products in luxor city, egypt
publisher	South Valley University
publishDate	2019
url	https://doaj.org/article/0f4f12b48a9e4a7d9f2aba9037698c2a
work_keys_str_mv	AT raniaiyounis detectionofmultidrugresistantfoodbornebacteriainreadytoeatmeatproductsinluxorcityegypt AT soadanasef detectionofmultidrugresistantfoodbornebacteriainreadytoeatmeatproductsinluxorcityegypt AT wesammsalem detectionofmultidrugresistantfoodbornebacteriainreadytoeatmeatproductsinluxorcityegypt
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Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt

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