STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.

Formalin fixed and paraffin-embedded human tissue resected during cancer surgery is indispensable for diagnostic and therapeutic purposes and represents a vast and largely unexploited resource for research. Optical microscopy of such specimen is curtailed by the diffraction-limited resolution of con...

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Autores principales:	Peter Ilgen, Stefan Stoldt, Lena-Christin Conradi, Christian Andreas Wurm, Josef Rüschoff, B Michael Ghadimi, Torsten Liersch, Stefan Jakobs
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f5155c0842d49838fc674b32a73b80e
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spelling	oai:doaj.org-article:0f5155c0842d49838fc674b32a73b80e2021-11-25T06:08:31ZSTED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.1932-620310.1371/journal.pone.0101563https://doaj.org/article/0f5155c0842d49838fc674b32a73b80e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25025184/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Formalin fixed and paraffin-embedded human tissue resected during cancer surgery is indispensable for diagnostic and therapeutic purposes and represents a vast and largely unexploited resource for research. Optical microscopy of such specimen is curtailed by the diffraction-limited resolution of conventional optical microscopy. To overcome this limitation, we used STED super-resolution microscopy enabling optical resolution well below the diffraction barrier. We visualized nanoscale protein distributions in sections of well-annotated paraffin-embedded human rectal cancer tissue stored in a clinical repository. Using antisera against several mitochondrial proteins, STED microscopy revealed distinct sub-mitochondrial protein distributions, suggesting a high level of structural preservation. Analysis of human tissues stored for up to 17 years demonstrated that these samples were still amenable for super-resolution microscopy. STED microscopy of sections of HER2 positive rectal adenocarcinoma revealed details in the surface and intracellular HER2 distribution that were blurred in the corresponding conventional images, demonstrating the potential of super-resolution microscopy to explore the thus far largely untapped nanoscale regime in tissues stored in biorepositories.Peter IlgenStefan StoldtLena-Christin ConradiChristian Andreas WurmJosef RüschoffB Michael GhadimiTorsten LierschStefan JakobsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e101563 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter Ilgen Stefan Stoldt Lena-Christin Conradi Christian Andreas Wurm Josef Rüschoff B Michael Ghadimi Torsten Liersch Stefan Jakobs STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
description	Formalin fixed and paraffin-embedded human tissue resected during cancer surgery is indispensable for diagnostic and therapeutic purposes and represents a vast and largely unexploited resource for research. Optical microscopy of such specimen is curtailed by the diffraction-limited resolution of conventional optical microscopy. To overcome this limitation, we used STED super-resolution microscopy enabling optical resolution well below the diffraction barrier. We visualized nanoscale protein distributions in sections of well-annotated paraffin-embedded human rectal cancer tissue stored in a clinical repository. Using antisera against several mitochondrial proteins, STED microscopy revealed distinct sub-mitochondrial protein distributions, suggesting a high level of structural preservation. Analysis of human tissues stored for up to 17 years demonstrated that these samples were still amenable for super-resolution microscopy. STED microscopy of sections of HER2 positive rectal adenocarcinoma revealed details in the surface and intracellular HER2 distribution that were blurred in the corresponding conventional images, demonstrating the potential of super-resolution microscopy to explore the thus far largely untapped nanoscale regime in tissues stored in biorepositories.
format	article
author	Peter Ilgen Stefan Stoldt Lena-Christin Conradi Christian Andreas Wurm Josef Rüschoff B Michael Ghadimi Torsten Liersch Stefan Jakobs
author_facet	Peter Ilgen Stefan Stoldt Lena-Christin Conradi Christian Andreas Wurm Josef Rüschoff B Michael Ghadimi Torsten Liersch Stefan Jakobs
author_sort	Peter Ilgen
title	STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
title_short	STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
title_full	STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
title_fullStr	STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
title_full_unstemmed	STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
title_sort	sted super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/0f5155c0842d49838fc674b32a73b80e
work_keys_str_mv	AT peterilgen stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT stefanstoldt stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT lenachristinconradi stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT christianandreaswurm stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT josefruschoff stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT bmichaelghadimi stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT torstenliersch stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue AT stefanjakobs stedsuperresolutionmicroscopyofclinicalparaffinembeddedhumanrectalcancertissue
_version_	1718414086427901952

STED super-resolution microscopy of clinical paraffin-embedded human rectal cancer tissue.

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