ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS

Stress-induced immune disregulation is a risk factor of autoimmune and inflammatory diseases, but, so far, the mechanisms for this effect are not fully known. Expression levels of specific mRNAs were assessed in gut-associated lymphoid tissue (GALT) from Wistar rats subjected to chronic social stres...

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Autores principales:	A. M. Kamyshnyi, I. A. Topol
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2015
Materias:	chronic social stress lymphoid tissue gut-associated gene expression nr3c1 adrβ2 il-1β il-17α nlrp3 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/0f59766eaae74b408b63796b10bbefa4
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spelling	oai:doaj.org-article:0f59766eaae74b408b63796b10bbefa42021-11-18T08:03:45ZACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS1563-06252313-741X10.15789/1563-0625-2015-5-455-460https://doaj.org/article/0f59766eaae74b408b63796b10bbefa42015-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/933https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XStress-induced immune disregulation is a risk factor of autoimmune and inflammatory diseases, but, so far, the mechanisms for this effect are not fully known. Expression levels of specific mRNAs were assessed in gut-associated lymphoid tissue (GALT) from Wistar rats subjected to chronic social stress (CSS). Gene expression was evaluated for NR3C1, Adrβ2, as well as IL-1β, IL-17α pro-inflammatory cytokines, and Nlrp, an inflammasome gene. Under the CSS conditions, we have shown altered distribution of RORγt +, FoxP3+, LMP2+, XBP1+ lymphocytes in GALT.The experiments were carried out with female Wistar rats aged 5–6 months. Specific mRNA expression for the target genes was determined by means of real-time PCR performed in a CFX96™ thermocycler («BioRadLaboratories, Inc»,USA). Relative levels of a target gene expression were quantified by the ΔΔCt method, being compared with rat GAPDH reference gene expression. Statistical analysis was performed with available «BioRad СFX Manager 3.1» software. Specific monoclonal rat antibodes were used for detection of immunopositive lymphocytes by means of indirect immunofluorescence technique.CSS development leads to decreased levels of mRNA expression for Nr3c1 and Adrβ2-genes in the GALT cells, being accompanied with unidirectional changes, i.e., increased transcription of pro-inflammatory cytokine mRNAs (IL-1β, IL-17α) and Nlrp3-inflammasome genes. These changes are accompanied by decreased FoxP3+/RORγt + cell ratio and predominant Th17 differentiation accompanied by suppressor failure. In addition, CSS development was characterized by unidirectional tendency for increasing total number of LMP2+ lymphocytes and reduced ХВР1+ cell population density in lymphoid structures of rat ileum.The events observed in GALT cell populations under CSS conditions are opposing classical paradigm of the stress response. The CSS-associated effects do not promote immunosuppression, however, are able to cause activation of immune system and inflammatory process.A. M. KamyshnyiI. A. TopolSPb RAACIarticlechronic social stresslymphoid tissuegut-associatedgene expressionnr3c1adrβ2il-1βil-17αnlrp3Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 5, Pp 455-460 (2015)
institution	DOAJ
collection	DOAJ
language	RU
topic	chronic social stress lymphoid tissue gut-associated gene expression nr3c1 adrβ2 il-1β il-17α nlrp3 Immunologic diseases. Allergy RC581-607
spellingShingle	chronic social stress lymphoid tissue gut-associated gene expression nr3c1 adrβ2 il-1β il-17α nlrp3 Immunologic diseases. Allergy RC581-607 A. M. Kamyshnyi I. A. Topol ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS
description	Stress-induced immune disregulation is a risk factor of autoimmune and inflammatory diseases, but, so far, the mechanisms for this effect are not fully known. Expression levels of specific mRNAs were assessed in gut-associated lymphoid tissue (GALT) from Wistar rats subjected to chronic social stress (CSS). Gene expression was evaluated for NR3C1, Adrβ2, as well as IL-1β, IL-17α pro-inflammatory cytokines, and Nlrp, an inflammasome gene. Under the CSS conditions, we have shown altered distribution of RORγt +, FoxP3+, LMP2+, XBP1+ lymphocytes in GALT.The experiments were carried out with female Wistar rats aged 5–6 months. Specific mRNA expression for the target genes was determined by means of real-time PCR performed in a CFX96™ thermocycler («BioRadLaboratories, Inc»,USA). Relative levels of a target gene expression were quantified by the ΔΔCt method, being compared with rat GAPDH reference gene expression. Statistical analysis was performed with available «BioRad СFX Manager 3.1» software. Specific monoclonal rat antibodes were used for detection of immunopositive lymphocytes by means of indirect immunofluorescence technique.CSS development leads to decreased levels of mRNA expression for Nr3c1 and Adrβ2-genes in the GALT cells, being accompanied with unidirectional changes, i.e., increased transcription of pro-inflammatory cytokine mRNAs (IL-1β, IL-17α) and Nlrp3-inflammasome genes. These changes are accompanied by decreased FoxP3+/RORγt + cell ratio and predominant Th17 differentiation accompanied by suppressor failure. In addition, CSS development was characterized by unidirectional tendency for increasing total number of LMP2+ lymphocytes and reduced ХВР1+ cell population density in lymphoid structures of rat ileum.The events observed in GALT cell populations under CSS conditions are opposing classical paradigm of the stress response. The CSS-associated effects do not promote immunosuppression, however, are able to cause activation of immune system and inflammatory process.
format	article
author	A. M. Kamyshnyi I. A. Topol
author_facet	A. M. Kamyshnyi I. A. Topol
author_sort	A. M. Kamyshnyi
title	ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS
title_short	ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS
title_full	ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS
title_fullStr	ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS
title_full_unstemmed	ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS
title_sort	activation mechanisms of gut-associated lymphoid tissue under chronic social stress conditions
publisher	SPb RAACI
publishDate	2015
url	https://doaj.org/article/0f59766eaae74b408b63796b10bbefa4
work_keys_str_mv	AT amkamyshnyi activationmechanismsofgutassociatedlymphoidtissueunderchronicsocialstressconditions AT iatopol activationmechanismsofgutassociatedlymphoidtissueunderchronicsocialstressconditions
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ACTIVATION MECHANISMS OF GUT-ASSOCIATED LYMPHOID TISSUE UNDER CHRONIC SOCIAL STRESS CONDITIONS

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