Legionella pneumophila secretes a mitochondrial carrier protein during infection.

The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite tra...

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Autores principales:	Pavel Dolezal, Margareta Aili, Janette Tong, Jhih-Hang Jiang, Carlo M T Marobbio, Sau Fung Lee, Ralf Schuelein, Simon Belluzzo, Eva Binova, Aurelie Mousnier, Gad Frankel, Giulia Giannuzzi, Ferdinando Palmieri, Kipros Gabriel, Thomas Naderer, Elizabeth L Hartland, Trevor Lithgow
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0f5d265e5d654a888748be2592c79cc4
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spelling	oai:doaj.org-article:0f5d265e5d654a888748be2592c79cc42021-11-18T06:04:54ZLegionella pneumophila secretes a mitochondrial carrier protein during infection.1553-73661553-737410.1371/journal.ppat.1002459https://doaj.org/article/0f5d265e5d654a888748be2592c79cc42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22241989/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. We discovered a novel MCF member, termed Legionellanucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease. LncP was secreted via the bacterial Dot/Icm type IV secretion system into macrophages and assembled in the mitochondrial inner membrane. In a yeast cellular system, LncP induced a dominant-negative phenotype that was rescued by deleting an endogenous ATP carrier. Substrate transport studies on purified LncP reconstituted in liposomes revealed that it catalyzes unidirectional transport and exchange of ATP transport across membranes, thereby supporting a role for LncP as an ATP transporter. A hidden Markov model revealed further MCF proteins in the intracellular pathogens, Legionella longbeachae and Neorickettsia sennetsu, thereby challenging the notion that MCF proteins exist exclusively in eukaryotic organisms.Pavel DolezalMargareta AiliJanette TongJhih-Hang JiangCarlo M T MarobbioSau Fung LeeRalf SchueleinSimon BelluzzoEva BinovaAurelie MousnierGad FrankelGiulia GiannuzziFerdinando PalmieriKipros GabrielThomas NadererElizabeth L HartlandTrevor LithgowPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 1, p e1002459 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Pavel Dolezal Margareta Aili Janette Tong Jhih-Hang Jiang Carlo M T Marobbio Sau Fung Lee Ralf Schuelein Simon Belluzzo Eva Binova Aurelie Mousnier Gad Frankel Giulia Giannuzzi Ferdinando Palmieri Kipros Gabriel Thomas Naderer Elizabeth L Hartland Trevor Lithgow Legionella pneumophila secretes a mitochondrial carrier protein during infection.
description	The Mitochondrial Carrier Family (MCF) is a signature group of integral membrane proteins that transport metabolites across the mitochondrial inner membrane in eukaryotes. MCF proteins are characterized by six transmembrane segments that assemble to form a highly-selective channel for metabolite transport. We discovered a novel MCF member, termed Legionellanucleotide carrier Protein (LncP), encoded in the genome of Legionella pneumophila, the causative agent of Legionnaire's disease. LncP was secreted via the bacterial Dot/Icm type IV secretion system into macrophages and assembled in the mitochondrial inner membrane. In a yeast cellular system, LncP induced a dominant-negative phenotype that was rescued by deleting an endogenous ATP carrier. Substrate transport studies on purified LncP reconstituted in liposomes revealed that it catalyzes unidirectional transport and exchange of ATP transport across membranes, thereby supporting a role for LncP as an ATP transporter. A hidden Markov model revealed further MCF proteins in the intracellular pathogens, Legionella longbeachae and Neorickettsia sennetsu, thereby challenging the notion that MCF proteins exist exclusively in eukaryotic organisms.
format	article
author	Pavel Dolezal Margareta Aili Janette Tong Jhih-Hang Jiang Carlo M T Marobbio Sau Fung Lee Ralf Schuelein Simon Belluzzo Eva Binova Aurelie Mousnier Gad Frankel Giulia Giannuzzi Ferdinando Palmieri Kipros Gabriel Thomas Naderer Elizabeth L Hartland Trevor Lithgow
author_facet	Pavel Dolezal Margareta Aili Janette Tong Jhih-Hang Jiang Carlo M T Marobbio Sau Fung Lee Ralf Schuelein Simon Belluzzo Eva Binova Aurelie Mousnier Gad Frankel Giulia Giannuzzi Ferdinando Palmieri Kipros Gabriel Thomas Naderer Elizabeth L Hartland Trevor Lithgow
author_sort	Pavel Dolezal
title	Legionella pneumophila secretes a mitochondrial carrier protein during infection.
title_short	Legionella pneumophila secretes a mitochondrial carrier protein during infection.
title_full	Legionella pneumophila secretes a mitochondrial carrier protein during infection.
title_fullStr	Legionella pneumophila secretes a mitochondrial carrier protein during infection.
title_full_unstemmed	Legionella pneumophila secretes a mitochondrial carrier protein during infection.
title_sort	legionella pneumophila secretes a mitochondrial carrier protein during infection.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0f5d265e5d654a888748be2592c79cc4
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Legionella pneumophila secretes a mitochondrial carrier protein during infection.

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