Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates

Abstract Carbon based polymer dots have piqued attention of researchers because of excellent biocompatibility, and good solubility. Most of the p-dots are able to generate ROS which is effective for photodynamic therapy for the treatment of cancer while some photosensitizers such as porphyrins posse...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Faiza Sajjad, Yi-Jia Yan, Davor Margetić, Zhi-Long Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f5fd02061fb4b1e81c89da4bcd158de
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0f5fd02061fb4b1e81c89da4bcd158de
record_format	dspace
spelling	oai:doaj.org-article:0f5fd02061fb4b1e81c89da4bcd158de2021-12-02T17:01:57ZSynthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates10.1038/s41598-021-89081-y2045-2322https://doaj.org/article/0f5fd02061fb4b1e81c89da4bcd158de2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89081-yhttps://doaj.org/toc/2045-2322Abstract Carbon based polymer dots have piqued attention of researchers because of excellent biocompatibility, and good solubility. Most of the p-dots are able to generate ROS which is effective for photodynamic therapy for the treatment of cancer while some photosensitizers such as porphyrins possess some drawbacks such as hydrophobicity, and dark toxicity. Therefore in this study we conjugated red emission carbon based polymer with pyropheophorbide-α through amide condensation and π–π stacking. One pot synthesis of the conjugate was successfully achieved. Their photophysiological properties were studied and structures were characterized by FT-IR, TEM and 1HNMR. pH- sensitivity of the conjugates was confirmed using fluorescence and UV–vis spectroscopy. Photo toxicity and dark toxicity of the prepared conjugates were evaluated in human esophageal cancer cell line (Eca-109). Hemocompatibility of the synthesized conjugates was evaluated and proved that the conjugates are safe to use for the treatment of tumor. Our results showed the PS doped p-dots had less dark toxicity and increased light toxicity as well as ROS generation was high as compared to precursor drug. Therefore, incorporation of p-dots to porphyrin improved biocompatibility and enhanced the photodynamic effect.Faiza SajjadYi-Jia YanDavor MargetićZhi-Long ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Faiza Sajjad Yi-Jia Yan Davor Margetić Zhi-Long Chen Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates
description	Abstract Carbon based polymer dots have piqued attention of researchers because of excellent biocompatibility, and good solubility. Most of the p-dots are able to generate ROS which is effective for photodynamic therapy for the treatment of cancer while some photosensitizers such as porphyrins possess some drawbacks such as hydrophobicity, and dark toxicity. Therefore in this study we conjugated red emission carbon based polymer with pyropheophorbide-α through amide condensation and π–π stacking. One pot synthesis of the conjugate was successfully achieved. Their photophysiological properties were studied and structures were characterized by FT-IR, TEM and 1HNMR. pH- sensitivity of the conjugates was confirmed using fluorescence and UV–vis spectroscopy. Photo toxicity and dark toxicity of the prepared conjugates were evaluated in human esophageal cancer cell line (Eca-109). Hemocompatibility of the synthesized conjugates was evaluated and proved that the conjugates are safe to use for the treatment of tumor. Our results showed the PS doped p-dots had less dark toxicity and increased light toxicity as well as ROS generation was high as compared to precursor drug. Therefore, incorporation of p-dots to porphyrin improved biocompatibility and enhanced the photodynamic effect.
format	article
author	Faiza Sajjad Yi-Jia Yan Davor Margetić Zhi-Long Chen
author_facet	Faiza Sajjad Yi-Jia Yan Davor Margetić Zhi-Long Chen
author_sort	Faiza Sajjad
title	Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates
title_short	Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates
title_full	Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates
title_fullStr	Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates
title_full_unstemmed	Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates
title_sort	synthesis and in vitro pdt evaluation of red emission polymer dots (r-cpds) and pyropheophorbide-α conjugates
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0f5fd02061fb4b1e81c89da4bcd158de
work_keys_str_mv	AT faizasajjad synthesisandinvitropdtevaluationofredemissionpolymerdotsrcpdsandpyropheophorbideaconjugates AT yijiayan synthesisandinvitropdtevaluationofredemissionpolymerdotsrcpdsandpyropheophorbideaconjugates AT davormargetic synthesisandinvitropdtevaluationofredemissionpolymerdotsrcpdsandpyropheophorbideaconjugates AT zhilongchen synthesisandinvitropdtevaluationofredemissionpolymerdotsrcpdsandpyropheophorbideaconjugates
_version_	1718381950743347200

Synthesis and in vitro PDT evaluation of red emission polymer dots (R-CPDs) and pyropheophorbide-α conjugates

Ejemplares similares