Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population

The first step toward marker-assisted selection is linking the phenotypes to molecular markers through quantitative trait loci (QTL) analysis. While the process is straightforward in self-pollinating diploid (2x) species, QTL analysis in polyploids requires unconventional methods. In this study, we...

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Autores principales:	Brigitte Uwimana, Gerald Mwanje, Michael Batte, Violet Akech, Trushar Shah, Marnik Vuylsteke, Rony Swennen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	banana banana weevil continuous mapping Cosmopolites sordidus Musa spp. polyploids Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/0f6ad23974594e7889fb8de5add3adca
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spelling	oai:doaj.org-article:0f6ad23974594e7889fb8de5add3adca2021-12-01T13:42:35ZContinuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population1664-462X10.3389/fpls.2021.753241https://doaj.org/article/0f6ad23974594e7889fb8de5add3adca2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.753241/fullhttps://doaj.org/toc/1664-462XThe first step toward marker-assisted selection is linking the phenotypes to molecular markers through quantitative trait loci (QTL) analysis. While the process is straightforward in self-pollinating diploid (2x) species, QTL analysis in polyploids requires unconventional methods. In this study, we have identified markers associated with weevil Cosmopolites sordidus (Germar) resistance in bananas using 138 triploid (2n = 3x) hybrids derived from a cross between a tetraploid “Monyet” (2n = 4x) and a 2x “Kokopo” (2n = 2x) banana genotypes. The population was genotyped by Diversity Arrays Technology Sequencing (DArTSeq), resulting in 18,009 polymorphic single nucleotide polymorphisms (SNPs) between the two parents. Marker–trait association was carried out by continuous mapping where the adjusted trait means for the corm peripheral damage (PD) and total cross-section damage (TXD), both on the logit scale, were regressed on the marker allele frequencies. Forty-four SNPs that were associated with corm PD were identified on the chromosomes 5, 6, and 8, with 41 of them located on chromosome 6 and segregated in “Kokopo.” Eleven SNPs associated with corm total TXD were identified on chromosome 6 and segregated in “Monyet.” The additive effect of replacing one reference allele with the alternative allele was determined at each marker position. The PD QTL was confirmed using conventional QTL linkage analysis in the simplex markers segregating in “Kokopo” (AAAA × RA). We also identified 43 putative genes in the vicinity of the markers significantly associated with the two traits. The identified loci associated with resistance to weevil damage will be used in the efforts of developing molecular tools for marker-assisted breeding in bananas.Brigitte UwimanaGerald MwanjeMichael BatteViolet AkechTrushar ShahMarnik VuylstekeRony SwennenRony SwennenFrontiers Media S.A.articlebananabanana weevilcontinuous mappingCosmopolites sordidusMusa spp.polyploidsPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	banana banana weevil continuous mapping Cosmopolites sordidus Musa spp. polyploids Plant culture SB1-1110
spellingShingle	banana banana weevil continuous mapping Cosmopolites sordidus Musa spp. polyploids Plant culture SB1-1110 Brigitte Uwimana Gerald Mwanje Michael Batte Violet Akech Trushar Shah Marnik Vuylsteke Rony Swennen Rony Swennen Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population
description	The first step toward marker-assisted selection is linking the phenotypes to molecular markers through quantitative trait loci (QTL) analysis. While the process is straightforward in self-pollinating diploid (2x) species, QTL analysis in polyploids requires unconventional methods. In this study, we have identified markers associated with weevil Cosmopolites sordidus (Germar) resistance in bananas using 138 triploid (2n = 3x) hybrids derived from a cross between a tetraploid “Monyet” (2n = 4x) and a 2x “Kokopo” (2n = 2x) banana genotypes. The population was genotyped by Diversity Arrays Technology Sequencing (DArTSeq), resulting in 18,009 polymorphic single nucleotide polymorphisms (SNPs) between the two parents. Marker–trait association was carried out by continuous mapping where the adjusted trait means for the corm peripheral damage (PD) and total cross-section damage (TXD), both on the logit scale, were regressed on the marker allele frequencies. Forty-four SNPs that were associated with corm PD were identified on the chromosomes 5, 6, and 8, with 41 of them located on chromosome 6 and segregated in “Kokopo.” Eleven SNPs associated with corm total TXD were identified on chromosome 6 and segregated in “Monyet.” The additive effect of replacing one reference allele with the alternative allele was determined at each marker position. The PD QTL was confirmed using conventional QTL linkage analysis in the simplex markers segregating in “Kokopo” (AAAA × RA). We also identified 43 putative genes in the vicinity of the markers significantly associated with the two traits. The identified loci associated with resistance to weevil damage will be used in the efforts of developing molecular tools for marker-assisted breeding in bananas.
format	article
author	Brigitte Uwimana Gerald Mwanje Michael Batte Violet Akech Trushar Shah Marnik Vuylsteke Rony Swennen Rony Swennen
author_facet	Brigitte Uwimana Gerald Mwanje Michael Batte Violet Akech Trushar Shah Marnik Vuylsteke Rony Swennen Rony Swennen
author_sort	Brigitte Uwimana
title	Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population
title_short	Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population
title_full	Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population
title_fullStr	Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population
title_full_unstemmed	Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population
title_sort	continuous mapping identifies loci associated with weevil resistance [cosmopolites sordidus (germar)] in a triploid banana population
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/0f6ad23974594e7889fb8de5add3adca
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Continuous Mapping Identifies Loci Associated With Weevil Resistance [Cosmopolites sordidus (Germar)] in a Triploid Banana Population

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