Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions

Abstract Aluminium salts such as aluminium chlorohydrate (ACH) are the active ingredients of antiperspirant products. Their mechanism of action involves a temporary and superficial plugging of eccrine sweat pores at the skin surface. We developed a microfluidic system that allows the real time obser...

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Autores principales:	Yasine Sakhawoth, Jules Dupire, Fabien Leonforte, Marion Chardon, Fabrice Monti, Patrick Tabeling, Bernard Cabane, Robert Botet, Jean-Baptiste Galey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f6ed9e018f34160aa8d0aaf03118994
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spelling	oai:doaj.org-article:0f6ed9e018f34160aa8d0aaf031189942021-12-02T13:18:08ZReal time observation of the interaction between aluminium salts and sweat under microfluidic conditions10.1038/s41598-021-85691-82045-2322https://doaj.org/article/0f6ed9e018f34160aa8d0aaf031189942021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85691-8https://doaj.org/toc/2045-2322Abstract Aluminium salts such as aluminium chlorohydrate (ACH) are the active ingredients of antiperspirant products. Their mechanism of action involves a temporary and superficial plugging of eccrine sweat pores at the skin surface. We developed a microfluidic system that allows the real time observation of the interactions between sweat and ACH in conditions mimicking physiological sweat flow and pore dimensions. Using artificial sweat containing bovine serum albumin as a model protein, we performed experiments under flowing conditions to demonstrate that pore clogging results from the aggregation of proteins by aluminium polycations at specific location in the sweat pore. Combining microfluidic experiments, confocal microscopy and numerical models helps to better understand the physical chemistry and mechanisms involved in pore plugging. The results show that plugging starts from the walls of sweat pores before expanding into the centre of the channel. The simulations aid in explaining the influence of ACH concentration as well as the impact of flow conditions on the localization of the plug. Altogether, these results outline the potential of both microfluidic confocal observations and numerical simulations at the single sweat pore level to understand why aluminium polycations are so efficient for sweat channel plugging.Yasine SakhawothJules DupireFabien LeonforteMarion ChardonFabrice MontiPatrick TabelingBernard CabaneRobert BotetJean-Baptiste GaleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yasine Sakhawoth Jules Dupire Fabien Leonforte Marion Chardon Fabrice Monti Patrick Tabeling Bernard Cabane Robert Botet Jean-Baptiste Galey Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
description	Abstract Aluminium salts such as aluminium chlorohydrate (ACH) are the active ingredients of antiperspirant products. Their mechanism of action involves a temporary and superficial plugging of eccrine sweat pores at the skin surface. We developed a microfluidic system that allows the real time observation of the interactions between sweat and ACH in conditions mimicking physiological sweat flow and pore dimensions. Using artificial sweat containing bovine serum albumin as a model protein, we performed experiments under flowing conditions to demonstrate that pore clogging results from the aggregation of proteins by aluminium polycations at specific location in the sweat pore. Combining microfluidic experiments, confocal microscopy and numerical models helps to better understand the physical chemistry and mechanisms involved in pore plugging. The results show that plugging starts from the walls of sweat pores before expanding into the centre of the channel. The simulations aid in explaining the influence of ACH concentration as well as the impact of flow conditions on the localization of the plug. Altogether, these results outline the potential of both microfluidic confocal observations and numerical simulations at the single sweat pore level to understand why aluminium polycations are so efficient for sweat channel plugging.
format	article
author	Yasine Sakhawoth Jules Dupire Fabien Leonforte Marion Chardon Fabrice Monti Patrick Tabeling Bernard Cabane Robert Botet Jean-Baptiste Galey
author_facet	Yasine Sakhawoth Jules Dupire Fabien Leonforte Marion Chardon Fabrice Monti Patrick Tabeling Bernard Cabane Robert Botet Jean-Baptiste Galey
author_sort	Yasine Sakhawoth
title	Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
title_short	Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
title_full	Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
title_fullStr	Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
title_full_unstemmed	Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
title_sort	real time observation of the interaction between aluminium salts and sweat under microfluidic conditions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0f6ed9e018f34160aa8d0aaf03118994
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Real time observation of the interaction between aluminium salts and sweat under microfluidic conditions

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