THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS

Abstract. The four chimeric recombinant antigens CBD-CFP10, CBD-ESAT6, ESAT6-CFP10 and CBD-P38 contained aminoacid sequences of full-size proteins ESAT6, CFP10 and matured protein P38 of M. tuberculosis, joined with aminoacid sequences of cellulose bind domain of endogluconase A (CBD) from Cellumona...

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Autores principales:	E. V. Vasilyeva, V. N. Verbov, A. B. Beklemishev, A. L. Mamaev, A. O. Tsyrul’nikov, T. A. Stepanenko, V. B. Ivanovskii, S. V. Lapin, Areg A. Totolian
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	m. tuberculosis e. coli synthetic genes cloning expression p38 esat6 cfp10 antigenic characteristics blood sera. Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/0f70ac820eee470aa7ae0aa463fd7ade
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spelling	oai:doaj.org-article:0f70ac820eee470aa7ae0aa463fd7ade2021-11-22T07:09:46ZTHE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS2220-76192313-739810.15789/2220-7619-2013-3-235-242https://doaj.org/article/0f70ac820eee470aa7ae0aa463fd7ade2014-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/135https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. The four chimeric recombinant antigens CBD-CFP10, CBD-ESAT6, ESAT6-CFP10 and CBD-P38 contained aminoacid sequences of full-size proteins ESAT6, CFP10 and matured protein P38 of M. tuberculosis, joined with aminoacid sequences of cellulose bind domain of endogluconase A (CBD) from Cellumonas fimi have been obtained by gene engineering methods. Recombinant proteins were purified by affine chromatography in column with Ni-NTA-sepharose 6В-CL and as PPDN-3 were used for detection of their antigenic activity in indirect ELISA for TB serological diagnostics. The sera from patients with lung tuberculosis (n = 321), from persons who had professional contacts with TB patients (n = 42), from healthy blood donors (n = 366) and from patients with lung diseases of non-TB etiology were tested. It was detected that there was positive correlation between antibodies level for all studied antigens compared by pair. It has been demonstrated that although antigens were different by antigenic and immunobiological characteristics they add each other in the content of antigenic diagnostics compositions. Thus, all these antigens can be used in the test kits for serological diagnostics of TB. Using of these antigens will allow to detect persons infected by TB and patients with active tuberculosis.E. V. VasilyevaV. N. VerbovA. B. BeklemishevA. L. MamaevA. O. Tsyrul’nikovT. A. StepanenkoV. B. IvanovskiiS. V. LapinAreg A. TotolianSankt-Peterburg : NIIÈM imeni Pasteraarticlem. tuberculosise. colisynthetic genescloningexpressionp38esat6cfp10antigenic characteristicsblood sera.Infectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 3, Iss 3, Pp 235-242 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	m. tuberculosis e. coli synthetic genes cloning expression p38 esat6 cfp10 antigenic characteristics blood sera. Infectious and parasitic diseases RC109-216
spellingShingle	m. tuberculosis e. coli synthetic genes cloning expression p38 esat6 cfp10 antigenic characteristics blood sera. Infectious and parasitic diseases RC109-216 E. V. Vasilyeva V. N. Verbov A. B. Beklemishev A. L. Mamaev A. O. Tsyrul’nikov T. A. Stepanenko V. B. Ivanovskii S. V. Lapin Areg A. Totolian THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS
description	Abstract. The four chimeric recombinant antigens CBD-CFP10, CBD-ESAT6, ESAT6-CFP10 and CBD-P38 contained aminoacid sequences of full-size proteins ESAT6, CFP10 and matured protein P38 of M. tuberculosis, joined with aminoacid sequences of cellulose bind domain of endogluconase A (CBD) from Cellumonas fimi have been obtained by gene engineering methods. Recombinant proteins were purified by affine chromatography in column with Ni-NTA-sepharose 6В-CL and as PPDN-3 were used for detection of their antigenic activity in indirect ELISA for TB serological diagnostics. The sera from patients with lung tuberculosis (n = 321), from persons who had professional contacts with TB patients (n = 42), from healthy blood donors (n = 366) and from patients with lung diseases of non-TB etiology were tested. It was detected that there was positive correlation between antibodies level for all studied antigens compared by pair. It has been demonstrated that although antigens were different by antigenic and immunobiological characteristics they add each other in the content of antigenic diagnostics compositions. Thus, all these antigens can be used in the test kits for serological diagnostics of TB. Using of these antigens will allow to detect persons infected by TB and patients with active tuberculosis.
format	article
author	E. V. Vasilyeva V. N. Verbov A. B. Beklemishev A. L. Mamaev A. O. Tsyrul’nikov T. A. Stepanenko V. B. Ivanovskii S. V. Lapin Areg A. Totolian
author_facet	E. V. Vasilyeva V. N. Verbov A. B. Beklemishev A. L. Mamaev A. O. Tsyrul’nikov T. A. Stepanenko V. B. Ivanovskii S. V. Lapin Areg A. Totolian
author_sort	E. V. Vasilyeva
title	THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS
title_short	THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS
title_full	THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS
title_fullStr	THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS
title_full_unstemmed	THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS
title_sort	search of optimal combination of antigens for serological diagnostics of tuberculosis
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/0f70ac820eee470aa7ae0aa463fd7ade
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THE SEARCH OF OPTIMAL COMBINATION OF ANTIGENS FOR SEROLOGICAL DIAGNOSTICS OF TUBERCULOSIS

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