Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya
Abstract Brucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. Th...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/0f7655385d1544abbdcda13d57441b69 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:0f7655385d1544abbdcda13d57441b69 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:0f7655385d1544abbdcda13d57441b692021-12-02T16:45:10ZMolecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya10.1038/s41598-021-88327-z2045-2322https://doaj.org/article/0f7655385d1544abbdcda13d57441b692021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88327-zhttps://doaj.org/toc/2045-2322Abstract Brucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. This study was conducted in multi-host livestock populations in Kenya to detect the circulating Brucella species and assess evidence of host–pathogen associations. Serum samples were collected from 228 cattle, 162 goats, 158 sheep, 49 camels, and 257 humans from Narok and Marsabit counties in Kenya. Information on age, location and history of abortion or retained placenta were obtained for sampled livestock. Data on age, gender and location of residence were also collected for human participants. All samples were tested using genus level real-time PCR assays with primers specific for IS711 and bcsp31 targets for the detection of Brucella. All genus positive samples (positive for both targets) were further tested with a speciation assay for AlkB and BMEI1162 targets, specific for B. abortus and B. melitensis, respectively. Samples with adequate quantities aggregating to 577 were also tested with the Rose Bengal Test (RBT). A total of 199 (33.3%) livestock and 99 (38.5%) human samples tested positive for genus Brucella. Animal Brucella PCR positive status was positively predicted by RBT positive results (OR = 8.3, 95% CI 4.0–17.1). Humans aged 21–40 years had higher odds (OR = 2.8, 95% CI 1.2–6.6) of being Brucella PCR positive compared to the other age categories. The data on detection of different Brucella species indicates that B. abortus was detected more often in cattle (OR = 2.3, 95% CI 1.1–4.6) and camels (OR = 2.9, 95% CI 1.3–6.3), while B. melitensis was detected more in sheep (OR = 3.6, 95% CI 2.0–6.7) and goats (OR = 1.7, 95% CI 1.0–3.1). Both B. abortus and B. melitensis DNA were detected in humans and in multiple livestock host species, suggesting cross-transmission of these species among the different hosts. The detection of these two zoonotic Brucella species in humans further underpins the importance of One Health prevention strategies that target multiple host species, especially in the multi-host livestock populations.James M. AkokoRoger PelleAbdulHamid S. LukambagireEunice M. MachukaDaniel NthiwaColetha MathewEric M. FèvreBernard BettElizabeth A. J. CookDoreen OtheroBassirou BonfohRudovick R. KazwalaGabriel ShirimaEsther SchellingJo E. B. HallidayCollins OumaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q James M. Akoko Roger Pelle AbdulHamid S. Lukambagire Eunice M. Machuka Daniel Nthiwa Coletha Mathew Eric M. Fèvre Bernard Bett Elizabeth A. J. Cook Doreen Othero Bassirou Bonfoh Rudovick R. Kazwala Gabriel Shirima Esther Schelling Jo E. B. Halliday Collins Ouma Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya |
| description |
Abstract Brucellosis, caused by several species of the genus Brucella, is a zoonotic disease that affects humans and animal species worldwide. Information on the Brucella species circulating in different hosts in Kenya is largely unknown, thus limiting the adoption of targeted control strategies. This study was conducted in multi-host livestock populations in Kenya to detect the circulating Brucella species and assess evidence of host–pathogen associations. Serum samples were collected from 228 cattle, 162 goats, 158 sheep, 49 camels, and 257 humans from Narok and Marsabit counties in Kenya. Information on age, location and history of abortion or retained placenta were obtained for sampled livestock. Data on age, gender and location of residence were also collected for human participants. All samples were tested using genus level real-time PCR assays with primers specific for IS711 and bcsp31 targets for the detection of Brucella. All genus positive samples (positive for both targets) were further tested with a speciation assay for AlkB and BMEI1162 targets, specific for B. abortus and B. melitensis, respectively. Samples with adequate quantities aggregating to 577 were also tested with the Rose Bengal Test (RBT). A total of 199 (33.3%) livestock and 99 (38.5%) human samples tested positive for genus Brucella. Animal Brucella PCR positive status was positively predicted by RBT positive results (OR = 8.3, 95% CI 4.0–17.1). Humans aged 21–40 years had higher odds (OR = 2.8, 95% CI 1.2–6.6) of being Brucella PCR positive compared to the other age categories. The data on detection of different Brucella species indicates that B. abortus was detected more often in cattle (OR = 2.3, 95% CI 1.1–4.6) and camels (OR = 2.9, 95% CI 1.3–6.3), while B. melitensis was detected more in sheep (OR = 3.6, 95% CI 2.0–6.7) and goats (OR = 1.7, 95% CI 1.0–3.1). Both B. abortus and B. melitensis DNA were detected in humans and in multiple livestock host species, suggesting cross-transmission of these species among the different hosts. The detection of these two zoonotic Brucella species in humans further underpins the importance of One Health prevention strategies that target multiple host species, especially in the multi-host livestock populations. |
| format |
article |
| author |
James M. Akoko Roger Pelle AbdulHamid S. Lukambagire Eunice M. Machuka Daniel Nthiwa Coletha Mathew Eric M. Fèvre Bernard Bett Elizabeth A. J. Cook Doreen Othero Bassirou Bonfoh Rudovick R. Kazwala Gabriel Shirima Esther Schelling Jo E. B. Halliday Collins Ouma |
| author_facet |
James M. Akoko Roger Pelle AbdulHamid S. Lukambagire Eunice M. Machuka Daniel Nthiwa Coletha Mathew Eric M. Fèvre Bernard Bett Elizabeth A. J. Cook Doreen Othero Bassirou Bonfoh Rudovick R. Kazwala Gabriel Shirima Esther Schelling Jo E. B. Halliday Collins Ouma |
| author_sort |
James M. Akoko |
| title |
Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya |
| title_short |
Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya |
| title_full |
Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya |
| title_fullStr |
Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya |
| title_full_unstemmed |
Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya |
| title_sort |
molecular epidemiology of brucella species in mixed livestock-human ecosystems in kenya |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/0f7655385d1544abbdcda13d57441b69 |
| work_keys_str_mv |
AT jamesmakoko molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT rogerpelle molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT abdulhamidslukambagire molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT eunicemmachuka molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT danielnthiwa molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT colethamathew molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT ericmfevre molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT bernardbett molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT elizabethajcook molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT doreenothero molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT bassiroubonfoh molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT rudovickrkazwala molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT gabrielshirima molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT estherschelling molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT joebhalliday molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya AT collinsouma molecularepidemiologyofbrucellaspeciesinmixedlivestockhumanecosystemsinkenya |
| _version_ |
1718383496999731200 |