First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture

Abstract A symbiosis of bacterial community (sometimes called microbiota) play essential roles in developmental life cycle and health of coral, starting since a larva. For examples, coral bacterial holobionts function nitrogen fixation, carbon supply, sulfur cycling and antibiotic production. Yet, a...

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Autores principales:	Chitrasak Kullapanich, Suppakarn Jandang, Matanee Palasuk, Voranop Viyakarn, Suchana Chavanich, Naraporn Somboonna
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f77a12ddf4f4121acad2c55da2ed42c
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spelling	oai:doaj.org-article:0f77a12ddf4f4121acad2c55da2ed42c2021-12-02T17:51:28ZFirst dynamics of bacterial community during development of Acropora humilis larvae in aquaculture10.1038/s41598-021-91379-w2045-2322https://doaj.org/article/0f77a12ddf4f4121acad2c55da2ed42c2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91379-whttps://doaj.org/toc/2045-2322Abstract A symbiosis of bacterial community (sometimes called microbiota) play essential roles in developmental life cycle and health of coral, starting since a larva. For examples, coral bacterial holobionts function nitrogen fixation, carbon supply, sulfur cycling and antibiotic production. Yet, a study of the dynamic of bacteria associated coral larvae development is complicated owning to a vast diversity and culturable difficulty of bacteria; hence this type of study remains unexplored for Acropora humilis larvae in Thai sea. This study represented the first to utilize 16S rRNA gene sequencing to describe the timely bacterial compositions during successfully cultured and reared A. humilis larval transformation in aquaculture (gametes were collected from Sattahip Bay, Chonburi province, Thailand), from gamete spawning (0 h) and fertilization stage (1 h), to embryonic cleavage (8 h), round cell development (28, 39 and 41 h), and planula formation (48 h). The sequencing results as estimated by Good’s coverage at genus level covered 99.65 ± 0.24% of total bacteria. While core phyla of bacteria were observed (Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes), changes in bacterial population structures and differential predominant core bacterial orders were denoted for each larval developmental stage, from fertilization to embryonic cleavage and subsequently from the embryonic cleavage to round cell development (P = 0.007). For instances, Pseudoalteromonas and Oceanospirillales were found prevalent at 8 h, and Rhizobiales were at 48 h. The bacterial population structures from the round cell stage, particularly at 41 h, showed gradual drift towards those of the planula formation stage, suggesting microbial selection. Overall, this study provides preliminary insights into the dynamics of bacterial community and their potentially functional association (estimated from the bacterial compositions) during the developmental embryonic A. humilis in a cultivation system in Southeast Asia region.Chitrasak KullapanichSuppakarn JandangMatanee PalasukVoranop ViyakarnSuchana ChavanichNaraporn SomboonnaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chitrasak Kullapanich Suppakarn Jandang Matanee Palasuk Voranop Viyakarn Suchana Chavanich Naraporn Somboonna First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture
description	Abstract A symbiosis of bacterial community (sometimes called microbiota) play essential roles in developmental life cycle and health of coral, starting since a larva. For examples, coral bacterial holobionts function nitrogen fixation, carbon supply, sulfur cycling and antibiotic production. Yet, a study of the dynamic of bacteria associated coral larvae development is complicated owning to a vast diversity and culturable difficulty of bacteria; hence this type of study remains unexplored for Acropora humilis larvae in Thai sea. This study represented the first to utilize 16S rRNA gene sequencing to describe the timely bacterial compositions during successfully cultured and reared A. humilis larval transformation in aquaculture (gametes were collected from Sattahip Bay, Chonburi province, Thailand), from gamete spawning (0 h) and fertilization stage (1 h), to embryonic cleavage (8 h), round cell development (28, 39 and 41 h), and planula formation (48 h). The sequencing results as estimated by Good’s coverage at genus level covered 99.65 ± 0.24% of total bacteria. While core phyla of bacteria were observed (Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes), changes in bacterial population structures and differential predominant core bacterial orders were denoted for each larval developmental stage, from fertilization to embryonic cleavage and subsequently from the embryonic cleavage to round cell development (P = 0.007). For instances, Pseudoalteromonas and Oceanospirillales were found prevalent at 8 h, and Rhizobiales were at 48 h. The bacterial population structures from the round cell stage, particularly at 41 h, showed gradual drift towards those of the planula formation stage, suggesting microbial selection. Overall, this study provides preliminary insights into the dynamics of bacterial community and their potentially functional association (estimated from the bacterial compositions) during the developmental embryonic A. humilis in a cultivation system in Southeast Asia region.
format	article
author	Chitrasak Kullapanich Suppakarn Jandang Matanee Palasuk Voranop Viyakarn Suchana Chavanich Naraporn Somboonna
author_facet	Chitrasak Kullapanich Suppakarn Jandang Matanee Palasuk Voranop Viyakarn Suchana Chavanich Naraporn Somboonna
author_sort	Chitrasak Kullapanich
title	First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture
title_short	First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture
title_full	First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture
title_fullStr	First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture
title_full_unstemmed	First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture
title_sort	first dynamics of bacterial community during development of acropora humilis larvae in aquaculture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0f77a12ddf4f4121acad2c55da2ed42c
work_keys_str_mv	AT chitrasakkullapanich firstdynamicsofbacterialcommunityduringdevelopmentofacroporahumilislarvaeinaquaculture AT suppakarnjandang firstdynamicsofbacterialcommunityduringdevelopmentofacroporahumilislarvaeinaquaculture AT mataneepalasuk firstdynamicsofbacterialcommunityduringdevelopmentofacroporahumilislarvaeinaquaculture AT voranopviyakarn firstdynamicsofbacterialcommunityduringdevelopmentofacroporahumilislarvaeinaquaculture AT suchanachavanich firstdynamicsofbacterialcommunityduringdevelopmentofacroporahumilislarvaeinaquaculture AT narapornsomboonna firstdynamicsofbacterialcommunityduringdevelopmentofacroporahumilislarvaeinaquaculture
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First dynamics of bacterial community during development of Acropora humilis larvae in aquaculture

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