Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats

Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats

Salvatore Valiante,1,* Annarita Falanga,2,3,* Luisa Cigliano,1 Giuseppina Iachetta,1 Rosa Anna Busiello,1 Valeria La Marca,1 Massimiliano Galdiero,4 Assunta Lombardi,1 Stefania Galdiero1,2 1Department of Biology, 2Department of Pharmacy, 3DFM Scarl, University of Naples Federico II, 4Depa...

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Autores principales: Valiante S, Falanga A, Cigliano L, Iachetta G, Busiello RA, La Marca V, Galdiero M, Lombardi A, Galdiero S
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/0f77f2749d2643e6b9445fdaf0da9613
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spelling oai:doaj.org-article:0f77f2749d2643e6b9445fdaf0da96132021-12-02T02:38:19ZPeptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats1178-2013https://doaj.org/article/0f77f2749d2643e6b9445fdaf0da96132015-03-01T00:00:00Zhttp://www.dovepress.com/peptide-gh625-enters-into-neuron-and-astrocyte-cell-lines-and-crosses--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Salvatore Valiante,1,* Annarita Falanga,2,3,* Luisa Cigliano,1 Giuseppina Iachetta,1 Rosa Anna Busiello,1 Valeria La Marca,1 Massimiliano Galdiero,4 Assunta Lombardi,1 Stefania Galdiero1,2 1Department of Biology, 2Department of Pharmacy, 3DFM Scarl, University of Naples Federico II, 4Department of Experimental Medicine, II University of Naples, Naples, Italy *These authors contributed equally to this paper and are considered joint first authors Abstract: Peptide gH625, derived from glycoprotein H of herpes simplex virus type 1, can enter cells efficiently and deliver a cargo. Nanoparticles armed with gH625 are able to cross an in vitro model of the blood–brain barrier (BBB). In the present study, in vitro experiments were performed to investigate whether gH625 can enter and accumulate in neuron and astrocyte cell lines. The ability of gH625 to cross the BBB in vivo was also evaluated. gH625 was administered in vivo to rats and its presence in the liver and in the brain was detected. Within 3.5 hours of intravenous administration, gH625 can be found beyond the BBB in proximity to cell neurites. gH625 has no toxic effects in vivo, since it does not affect the maximal oxidative capacity of the brain or the mitochondrial respiration rate. Our data suggest that gH625, with its ability to cross the BBB, represents a novel nanocarrier system for drug delivery to the central nervous system. These results open up new possibilities for direct delivery of drugs into patients in the field of theranostics and might address the treatment of several human diseases. Keywords: drug delivery, neurons, astrocytes, blood–brain barrier, peptideValiante SFalanga ACigliano LIachetta GBusiello RALa Marca VGaldiero MLombardi AGaldiero SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1885-1898 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Valiante S
Falanga A
Cigliano L
Iachetta G
Busiello RA
La Marca V
Galdiero M
Lombardi A
Galdiero S
Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
description Salvatore Valiante,1,* Annarita Falanga,2,3,* Luisa Cigliano,1 Giuseppina Iachetta,1 Rosa Anna Busiello,1 Valeria La Marca,1 Massimiliano Galdiero,4 Assunta Lombardi,1 Stefania Galdiero1,2 1Department of Biology, 2Department of Pharmacy, 3DFM Scarl, University of Naples Federico II, 4Department of Experimental Medicine, II University of Naples, Naples, Italy *These authors contributed equally to this paper and are considered joint first authors Abstract: Peptide gH625, derived from glycoprotein H of herpes simplex virus type 1, can enter cells efficiently and deliver a cargo. Nanoparticles armed with gH625 are able to cross an in vitro model of the blood–brain barrier (BBB). In the present study, in vitro experiments were performed to investigate whether gH625 can enter and accumulate in neuron and astrocyte cell lines. The ability of gH625 to cross the BBB in vivo was also evaluated. gH625 was administered in vivo to rats and its presence in the liver and in the brain was detected. Within 3.5 hours of intravenous administration, gH625 can be found beyond the BBB in proximity to cell neurites. gH625 has no toxic effects in vivo, since it does not affect the maximal oxidative capacity of the brain or the mitochondrial respiration rate. Our data suggest that gH625, with its ability to cross the BBB, represents a novel nanocarrier system for drug delivery to the central nervous system. These results open up new possibilities for direct delivery of drugs into patients in the field of theranostics and might address the treatment of several human diseases. Keywords: drug delivery, neurons, astrocytes, blood–brain barrier, peptide
format article
author Valiante S
Falanga A
Cigliano L
Iachetta G
Busiello RA
La Marca V
Galdiero M
Lombardi A
Galdiero S
author_facet Valiante S
Falanga A
Cigliano L
Iachetta G
Busiello RA
La Marca V
Galdiero M
Lombardi A
Galdiero S
author_sort Valiante S
title Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
title_short Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
title_full Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
title_fullStr Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
title_full_unstemmed Peptide gH625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
title_sort peptide gh625 enters into neuron and astrocyte cell lines and crosses the blood–brain barrier in rats
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/0f77f2749d2643e6b9445fdaf0da9613
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AT ciglianol peptidegh625entersintoneuronandastrocytecelllinesandcrossesthebloodndashbrainbarrierinnbsprats
AT iachettag peptidegh625entersintoneuronandastrocytecelllinesandcrossesthebloodndashbrainbarrierinnbsprats
AT busiellora peptidegh625entersintoneuronandastrocytecelllinesandcrossesthebloodndashbrainbarrierinnbsprats
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AT lombardia peptidegh625entersintoneuronandastrocytecelllinesandcrossesthebloodndashbrainbarrierinnbsprats
AT galdieros peptidegh625entersintoneuronandastrocytecelllinesandcrossesthebloodndashbrainbarrierinnbsprats
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