Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.

Small noncoding RNAs (sRNA) can function as posttranscriptional activators of gene expression to regulate stress responses and metabolism. We here describe the mechanisms by which two sRNAs, GlmY and GlmZ, activate the Escherichia coli glmS mRNA, coding for an essential enzyme in amino-sugar metabol...

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Autores principales:	Johannes H Urban, Jörg Vogel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0f846a8aca55495e90d9782e91ff4005
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spelling	oai:doaj.org-article:0f846a8aca55495e90d9782e91ff40052021-11-25T05:33:26ZTwo seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.1544-91731545-788510.1371/journal.pbio.0060064https://doaj.org/article/0f846a8aca55495e90d9782e91ff40052008-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18351803/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Small noncoding RNAs (sRNA) can function as posttranscriptional activators of gene expression to regulate stress responses and metabolism. We here describe the mechanisms by which two sRNAs, GlmY and GlmZ, activate the Escherichia coli glmS mRNA, coding for an essential enzyme in amino-sugar metabolism. The two sRNAs, although being highly similar in sequence and structure, act in a hierarchical manner. GlmZ, together with the RNA chaperone, Hfq, directly activates glmS mRNA translation by an anti-antisense mechanism. In contrast, GlmY acts upstream of GlmZ and positively regulates glmS by antagonizing GlmZ RNA inactivation. We also report the first example, to our knowledge, of mRNA expression being controlled by the poly(A) status of a chromosomally encoded sRNA. We show that in wild-type cells, GlmY RNA is unstable due to 3' end polyadenylation; whereas in an E. coli pcnB mutant defective in RNA polyadenylation, GlmY is stabilized and accumulates, which in turn stabilizes GlmZ and causes GlmS overproduction. Our study reveals hierarchical action of two well-conserved sRNAs in a complex regulatory cascade that controls the glmS mRNA. Similar cascades of noncoding RNA regulators may operate in other organisms.Johannes H UrbanJörg VogelPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 3, p e64 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Johannes H Urban Jörg Vogel Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
description	Small noncoding RNAs (sRNA) can function as posttranscriptional activators of gene expression to regulate stress responses and metabolism. We here describe the mechanisms by which two sRNAs, GlmY and GlmZ, activate the Escherichia coli glmS mRNA, coding for an essential enzyme in amino-sugar metabolism. The two sRNAs, although being highly similar in sequence and structure, act in a hierarchical manner. GlmZ, together with the RNA chaperone, Hfq, directly activates glmS mRNA translation by an anti-antisense mechanism. In contrast, GlmY acts upstream of GlmZ and positively regulates glmS by antagonizing GlmZ RNA inactivation. We also report the first example, to our knowledge, of mRNA expression being controlled by the poly(A) status of a chromosomally encoded sRNA. We show that in wild-type cells, GlmY RNA is unstable due to 3' end polyadenylation; whereas in an E. coli pcnB mutant defective in RNA polyadenylation, GlmY is stabilized and accumulates, which in turn stabilizes GlmZ and causes GlmS overproduction. Our study reveals hierarchical action of two well-conserved sRNAs in a complex regulatory cascade that controls the glmS mRNA. Similar cascades of noncoding RNA regulators may operate in other organisms.
format	article
author	Johannes H Urban Jörg Vogel
author_facet	Johannes H Urban Jörg Vogel
author_sort	Johannes H Urban
title	Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
title_short	Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
title_full	Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
title_fullStr	Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
title_full_unstemmed	Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.
title_sort	two seemingly homologous noncoding rnas act hierarchically to activate glms mrna translation.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/0f846a8aca55495e90d9782e91ff4005
work_keys_str_mv	AT johanneshurban twoseeminglyhomologousnoncodingrnasacthierarchicallytoactivateglmsmrnatranslation AT jorgvogel twoseeminglyhomologousnoncodingrnasacthierarchicallytoactivateglmsmrnatranslation
_version_	1718414620427812864

Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation.

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