Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing
Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed...
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2021
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oai:doaj.org-article:0f86879ad39a45a58718f743ba9bcf082021-11-16T05:38:43ZIdentification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing1664-462X10.3389/fpls.2021.703520https://doaj.org/article/0f86879ad39a45a58718f743ba9bcf082021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.703520/fullhttps://doaj.org/toc/1664-462XClubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.Fuquan CeFuquan CeFuquan CeFuquan CeJiaqin MeiJiaqin MeiJiaqin MeiHaiyan HeHaiyan HeHaiyan HeYu ZhaoYu ZhaoWenhui HuWenhui HuWenhui HuFengqun YuQinfei LiQinfei LiQinfei LiXuesong RenXuesong RenXuesong RenJun SiJun SiJun SiHongyuan SongHongyuan SongHongyuan SongWei QianWei QianWei QianFrontiers Media S.A.articleBrassica oleraceaPlasmodiophora brassicaeclubrootgenomic resequencingcandidate genefunctional markerPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
Brassica oleracea Plasmodiophora brassicae clubroot genomic resequencing candidate gene functional marker Plant culture SB1-1110 |
| spellingShingle |
Brassica oleracea Plasmodiophora brassicae clubroot genomic resequencing candidate gene functional marker Plant culture SB1-1110 Fuquan Ce Fuquan Ce Fuquan Ce Fuquan Ce Jiaqin Mei Jiaqin Mei Jiaqin Mei Haiyan He Haiyan He Haiyan He Yu Zhao Yu Zhao Wenhui Hu Wenhui Hu Wenhui Hu Fengqun Yu Qinfei Li Qinfei Li Qinfei Li Xuesong Ren Xuesong Ren Xuesong Ren Jun Si Jun Si Jun Si Hongyuan Song Hongyuan Song Hongyuan Song Wei Qian Wei Qian Wei Qian Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing |
| description |
Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR. |
| format |
article |
| author |
Fuquan Ce Fuquan Ce Fuquan Ce Fuquan Ce Jiaqin Mei Jiaqin Mei Jiaqin Mei Haiyan He Haiyan He Haiyan He Yu Zhao Yu Zhao Wenhui Hu Wenhui Hu Wenhui Hu Fengqun Yu Qinfei Li Qinfei Li Qinfei Li Xuesong Ren Xuesong Ren Xuesong Ren Jun Si Jun Si Jun Si Hongyuan Song Hongyuan Song Hongyuan Song Wei Qian Wei Qian Wei Qian |
| author_facet |
Fuquan Ce Fuquan Ce Fuquan Ce Fuquan Ce Jiaqin Mei Jiaqin Mei Jiaqin Mei Haiyan He Haiyan He Haiyan He Yu Zhao Yu Zhao Wenhui Hu Wenhui Hu Wenhui Hu Fengqun Yu Qinfei Li Qinfei Li Qinfei Li Xuesong Ren Xuesong Ren Xuesong Ren Jun Si Jun Si Jun Si Hongyuan Song Hongyuan Song Hongyuan Song Wei Qian Wei Qian Wei Qian |
| author_sort |
Fuquan Ce |
| title |
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing |
| title_short |
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing |
| title_full |
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing |
| title_fullStr |
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing |
| title_full_unstemmed |
Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing |
| title_sort |
identification of candidate genes for clubroot-resistance in brassica oleracea using quantitative trait loci-sequencing |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/0f86879ad39a45a58718f743ba9bcf08 |
| work_keys_str_mv |
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