Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor

Abstract Epigallocatechin-3-gallate (EGCG) is one of the major polyphenolic compounds present in green tea extracts and has been used as a potential drug for the treatment of numerous diseases. The present study aimed to elucidate the role and mechanism of EGCG in protecting against H2O2-induced apo...

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Autores principales: Xue Yan, Yanfei Li, Han Yu, Wei Wang, Chunyan Wu, Yang Yang, Yongjia Hu, Xiujuan Shi, Jue Li
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/0f869f61ed504f27b8f4fd7d3fcf02d2
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spelling oai:doaj.org-article:0f869f61ed504f27b8f4fd7d3fcf02d22021-12-02T12:32:27ZEpigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor10.1038/s41598-017-08301-62045-2322https://doaj.org/article/0f869f61ed504f27b8f4fd7d3fcf02d22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08301-6https://doaj.org/toc/2045-2322Abstract Epigallocatechin-3-gallate (EGCG) is one of the major polyphenolic compounds present in green tea extracts and has been used as a potential drug for the treatment of numerous diseases. The present study aimed to elucidate the role and mechanism of EGCG in protecting against H2O2-induced apoptosis in mouse vascular smooth muscle cells (VSMCs). VSMCs were pretreated with various concentrations of EGCG for 2 hours prior to treatment with H2O2. Treatment with H2O2 significantly decreased the cell viability and induced apoptosis of VSMCs, which were attenuated by pretreatment with EGCG. In particular, EGCG pretreatment significantly inhibited the H2O2-induced upregulation of cleaved forms of caspase-3, caspase-8, and caspase-9, Bax, CathepsinD, and downregulation of Bcl-2. Moreover, the antioxidation effect of EGCG on VSMCs was determined to be associated with the 67kD laminin receptor (67LR). Our results demonstrated that EGCG improved cell viability and protected VSMCs against oxidative stress through both extrinsic and intrinsic pathways, while 67LR is likely to be an active and key receptor of EGCG. These findings provide a novel molecular mechanism of EGCG in inhibiting H2O2-induced apoptosis in VSMCs, as well as its function in preventing the development of atherosclerosis.Xue YanYanfei LiHan YuWei WangChunyan WuYang YangYongjia HuXiujuan ShiJue LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xue Yan
Yanfei Li
Han Yu
Wei Wang
Chunyan Wu
Yang Yang
Yongjia Hu
Xiujuan Shi
Jue Li
Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor
description Abstract Epigallocatechin-3-gallate (EGCG) is one of the major polyphenolic compounds present in green tea extracts and has been used as a potential drug for the treatment of numerous diseases. The present study aimed to elucidate the role and mechanism of EGCG in protecting against H2O2-induced apoptosis in mouse vascular smooth muscle cells (VSMCs). VSMCs were pretreated with various concentrations of EGCG for 2 hours prior to treatment with H2O2. Treatment with H2O2 significantly decreased the cell viability and induced apoptosis of VSMCs, which were attenuated by pretreatment with EGCG. In particular, EGCG pretreatment significantly inhibited the H2O2-induced upregulation of cleaved forms of caspase-3, caspase-8, and caspase-9, Bax, CathepsinD, and downregulation of Bcl-2. Moreover, the antioxidation effect of EGCG on VSMCs was determined to be associated with the 67kD laminin receptor (67LR). Our results demonstrated that EGCG improved cell viability and protected VSMCs against oxidative stress through both extrinsic and intrinsic pathways, while 67LR is likely to be an active and key receptor of EGCG. These findings provide a novel molecular mechanism of EGCG in inhibiting H2O2-induced apoptosis in VSMCs, as well as its function in preventing the development of atherosclerosis.
format article
author Xue Yan
Yanfei Li
Han Yu
Wei Wang
Chunyan Wu
Yang Yang
Yongjia Hu
Xiujuan Shi
Jue Li
author_facet Xue Yan
Yanfei Li
Han Yu
Wei Wang
Chunyan Wu
Yang Yang
Yongjia Hu
Xiujuan Shi
Jue Li
author_sort Xue Yan
title Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor
title_short Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor
title_full Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor
title_fullStr Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor
title_full_unstemmed Epigallocatechin-3-gallate inhibits H2O2-induced apoptosis in Mouse Vascular Smooth Muscle Cells via 67kD Laminin Receptor
title_sort epigallocatechin-3-gallate inhibits h2o2-induced apoptosis in mouse vascular smooth muscle cells via 67kd laminin receptor
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/0f869f61ed504f27b8f4fd7d3fcf02d2
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