Local Response to Microneedle-Based Influenza Immunization in the Skin

ABSTRACT Microneedle patches (MN) provide a novel method of vaccine delivery to the skin with the objective of targeting the large network of resident antigen-presenting cells to induce an efficient immune response. Our previous reports demonstrated that cutaneous delivery of inactivated influenza v...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maria del Pilar Martin, William C. Weldon, Vladimir G. Zarnitsyn, Dimitrios G. Koutsonanos, Hamed Akbari, Ioanna Skountzou, Joshy Jacob, Mark R. Prausnitz, Richard W. Compans
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2012
Materias:
Acceso en línea:https://doaj.org/article/0f8e0295880549288b936d74718676b4
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
Descripción
Sumario:ABSTRACT Microneedle patches (MN) provide a novel method of vaccine delivery to the skin with the objective of targeting the large network of resident antigen-presenting cells to induce an efficient immune response. Our previous reports demonstrated that cutaneous delivery of inactivated influenza virus-coated MN to mice protects against lethal infection. Protection is correlated with sustained levels of anti-influenza virus serum antibodies, hemagglutination inhibition titers, and robust cellular responses that are often stronger than those generated by intramuscular vaccination. Here we dissect the early events occurring in murine skin after microneedle delivery of inactivated influenza virus. We demonstrate correlation of immunization against influenza virus with a local increase of cytokines important for recruitment of neutrophils, monocytes and dendritic cells at the site of immunization. We also observed prolonged antigen deposition, and migration of matured dendritic cells bearing influenza virus antigen from the skin. IMPORTANCE The immunological mechanisms by which MN vaccination confers protective immunity are not well understood. The present study provides a first analysis of the early immune events after microneedle-based vaccination.