Local Response to Microneedle-Based Influenza Immunization in the Skin

ABSTRACT Microneedle patches (MN) provide a novel method of vaccine delivery to the skin with the objective of targeting the large network of resident antigen-presenting cells to induce an efficient immune response. Our previous reports demonstrated that cutaneous delivery of inactivated influenza v...

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Autores principales:	Maria del Pilar Martin, William C. Weldon, Vladimir G. Zarnitsyn, Dimitrios G. Koutsonanos, Hamed Akbari, Ioanna Skountzou, Joshy Jacob, Mark R. Prausnitz, Richard W. Compans
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2012
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/0f8e0295880549288b936d74718676b4
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spelling	oai:doaj.org-article:0f8e0295880549288b936d74718676b42021-11-15T15:39:08ZLocal Response to Microneedle-Based Influenza Immunization in the Skin10.1128/mBio.00012-122150-7511https://doaj.org/article/0f8e0295880549288b936d74718676b42012-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00012-12https://doaj.org/toc/2150-7511ABSTRACT Microneedle patches (MN) provide a novel method of vaccine delivery to the skin with the objective of targeting the large network of resident antigen-presenting cells to induce an efficient immune response. Our previous reports demonstrated that cutaneous delivery of inactivated influenza virus-coated MN to mice protects against lethal infection. Protection is correlated with sustained levels of anti-influenza virus serum antibodies, hemagglutination inhibition titers, and robust cellular responses that are often stronger than those generated by intramuscular vaccination. Here we dissect the early events occurring in murine skin after microneedle delivery of inactivated influenza virus. We demonstrate correlation of immunization against influenza virus with a local increase of cytokines important for recruitment of neutrophils, monocytes and dendritic cells at the site of immunization. We also observed prolonged antigen deposition, and migration of matured dendritic cells bearing influenza virus antigen from the skin. IMPORTANCE The immunological mechanisms by which MN vaccination confers protective immunity are not well understood. The present study provides a first analysis of the early immune events after microneedle-based vaccination.Maria del Pilar MartinWilliam C. WeldonVladimir G. ZarnitsynDimitrios G. KoutsonanosHamed AkbariIoanna SkountzouJoshy JacobMark R. PrausnitzRichard W. CompansAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 3, Iss 2 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Maria del Pilar Martin William C. Weldon Vladimir G. Zarnitsyn Dimitrios G. Koutsonanos Hamed Akbari Ioanna Skountzou Joshy Jacob Mark R. Prausnitz Richard W. Compans Local Response to Microneedle-Based Influenza Immunization in the Skin
description	ABSTRACT Microneedle patches (MN) provide a novel method of vaccine delivery to the skin with the objective of targeting the large network of resident antigen-presenting cells to induce an efficient immune response. Our previous reports demonstrated that cutaneous delivery of inactivated influenza virus-coated MN to mice protects against lethal infection. Protection is correlated with sustained levels of anti-influenza virus serum antibodies, hemagglutination inhibition titers, and robust cellular responses that are often stronger than those generated by intramuscular vaccination. Here we dissect the early events occurring in murine skin after microneedle delivery of inactivated influenza virus. We demonstrate correlation of immunization against influenza virus with a local increase of cytokines important for recruitment of neutrophils, monocytes and dendritic cells at the site of immunization. We also observed prolonged antigen deposition, and migration of matured dendritic cells bearing influenza virus antigen from the skin. IMPORTANCE The immunological mechanisms by which MN vaccination confers protective immunity are not well understood. The present study provides a first analysis of the early immune events after microneedle-based vaccination.
format	article
author	Maria del Pilar Martin William C. Weldon Vladimir G. Zarnitsyn Dimitrios G. Koutsonanos Hamed Akbari Ioanna Skountzou Joshy Jacob Mark R. Prausnitz Richard W. Compans
author_facet	Maria del Pilar Martin William C. Weldon Vladimir G. Zarnitsyn Dimitrios G. Koutsonanos Hamed Akbari Ioanna Skountzou Joshy Jacob Mark R. Prausnitz Richard W. Compans
author_sort	Maria del Pilar Martin
title	Local Response to Microneedle-Based Influenza Immunization in the Skin
title_short	Local Response to Microneedle-Based Influenza Immunization in the Skin
title_full	Local Response to Microneedle-Based Influenza Immunization in the Skin
title_fullStr	Local Response to Microneedle-Based Influenza Immunization in the Skin
title_full_unstemmed	Local Response to Microneedle-Based Influenza Immunization in the Skin
title_sort	local response to microneedle-based influenza immunization in the skin
publisher	American Society for Microbiology
publishDate	2012
url	https://doaj.org/article/0f8e0295880549288b936d74718676b4
work_keys_str_mv	AT mariadelpilarmartin localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT williamcweldon localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT vladimirgzarnitsyn localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT dimitriosgkoutsonanos localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT hamedakbari localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT ioannaskountzou localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT joshyjacob localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT markrprausnitz localresponsetomicroneedlebasedinfluenzaimmunizationintheskin AT richardwcompans localresponsetomicroneedlebasedinfluenzaimmunizationintheskin
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Local Response to Microneedle-Based Influenza Immunization in the Skin

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