Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

Abstract In the eye, intraocular pressure (IOP) is tightly regulated and its persistent increase leads to ocular hypertension and glaucoma. We have previously shown that trabecular meshwork (TM) cells might detect aqueous humor fluid shear stress via interaction of the extracellular matrix (ECM) pro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Teresia A. Carreon, Aida Castellanos, Xavier Gasull, Sanjoy K. Bhattacharya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0f9f8ab4fd0649ebb60fa5214f2cdc00
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0f9f8ab4fd0649ebb60fa5214f2cdc00
record_format	dspace
spelling	oai:doaj.org-article:0f9f8ab4fd0649ebb60fa5214f2cdc002021-12-02T11:52:15ZInteraction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure10.1038/s41598-017-00430-22045-2322https://doaj.org/article/0f9f8ab4fd0649ebb60fa5214f2cdc002017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00430-2https://doaj.org/toc/2045-2322Abstract In the eye, intraocular pressure (IOP) is tightly regulated and its persistent increase leads to ocular hypertension and glaucoma. We have previously shown that trabecular meshwork (TM) cells might detect aqueous humor fluid shear stress via interaction of the extracellular matrix (ECM) protein cochlin with the cell surface bound and stretch-activated channel TREK-1. We provide evidence here that interaction between both proteins are involved in IOP regulation. Silencing of TREK-1 in mice prevents the previously demonstrated cochlin-overexpression mediated increase in IOP. Biochemical and electrophysiological experiments demonstrate that high shear stress-induced multimeric cochlin produces a qualitatively different interaction with TREK-1 compared to monomeric cochlin. Physiological concentrations of multimeric but not monomeric cochlin reduce TREK-1 current. Results presented here indicate that the interaction of TREK-1 and cochlin play an important role for maintaining IOP homeostasis.Teresia A. CarreonAida CastellanosXavier GasullSanjoy K. BhattacharyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teresia A. Carreon Aida Castellanos Xavier Gasull Sanjoy K. Bhattacharya Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
description	Abstract In the eye, intraocular pressure (IOP) is tightly regulated and its persistent increase leads to ocular hypertension and glaucoma. We have previously shown that trabecular meshwork (TM) cells might detect aqueous humor fluid shear stress via interaction of the extracellular matrix (ECM) protein cochlin with the cell surface bound and stretch-activated channel TREK-1. We provide evidence here that interaction between both proteins are involved in IOP regulation. Silencing of TREK-1 in mice prevents the previously demonstrated cochlin-overexpression mediated increase in IOP. Biochemical and electrophysiological experiments demonstrate that high shear stress-induced multimeric cochlin produces a qualitatively different interaction with TREK-1 compared to monomeric cochlin. Physiological concentrations of multimeric but not monomeric cochlin reduce TREK-1 current. Results presented here indicate that the interaction of TREK-1 and cochlin play an important role for maintaining IOP homeostasis.
format	article
author	Teresia A. Carreon Aida Castellanos Xavier Gasull Sanjoy K. Bhattacharya
author_facet	Teresia A. Carreon Aida Castellanos Xavier Gasull Sanjoy K. Bhattacharya
author_sort	Teresia A. Carreon
title	Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
title_short	Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
title_full	Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
title_fullStr	Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
title_full_unstemmed	Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
title_sort	interaction of cochlin and mechanosensitive channel trek-1 in trabecular meshwork cells influences the regulation of intraocular pressure
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0f9f8ab4fd0649ebb60fa5214f2cdc00
work_keys_str_mv	AT teresiaacarreon interactionofcochlinandmechanosensitivechanneltrek1intrabecularmeshworkcellsinfluencestheregulationofintraocularpressure AT aidacastellanos interactionofcochlinandmechanosensitivechanneltrek1intrabecularmeshworkcellsinfluencestheregulationofintraocularpressure AT xaviergasull interactionofcochlinandmechanosensitivechanneltrek1intrabecularmeshworkcellsinfluencestheregulationofintraocularpressure AT sanjoykbhattacharya interactionofcochlinandmechanosensitivechanneltrek1intrabecularmeshworkcellsinfluencestheregulationofintraocularpressure
_version_	1718395086998339584

Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure

Ejemplares similares