Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress

Abstract Pulmonary fibrosis is a kind of devastating interstitial lung disease due to the limited therapeutic strategies. Traditional Chinese medicine (TCM) practices have put forth Shenks as a promising treatment approach. Here, we performed in vivo study and in vitro study to delineate the anti-fi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Haiyan Chu, Ying Shi, Shuai Jiang, Qicheng Zhong, Yongqiang Zhao, Qingmei Liu, Yanyun Ma, Xiangguang Shi, Weifeng Ding, Xiaodong Zhou, Jimin Cui, Li Jin, Gang Guo, Jiucun Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0facbc85898245e5ac42ab18e616186d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0facbc85898245e5ac42ab18e616186d
record_format	dspace
spelling	oai:doaj.org-article:0facbc85898245e5ac42ab18e616186d2021-12-02T11:51:11ZTreatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress10.1038/s41598-017-02293-z2045-2322https://doaj.org/article/0facbc85898245e5ac42ab18e616186d2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02293-zhttps://doaj.org/toc/2045-2322Abstract Pulmonary fibrosis is a kind of devastating interstitial lung disease due to the limited therapeutic strategies. Traditional Chinese medicine (TCM) practices have put forth Shenks as a promising treatment approach. Here, we performed in vivo study and in vitro study to delineate the anti-fibrotic mechanisms behind Shenks treatment for pulmonary fibrosis. We found that regardless of the prophylactic or therapeutic treatment, Shenks was able to attenuate BLM-induced-fibrosis in mice, down regulate extracellular matrix genes expression, and reduce collagen production. The aberrantly high Smad3 phosphorylation levels and SBE activity in TGF-β-induced fibroblasts were dramatically decreased as a result of Shenks treatment. At the same time, Shenks was able to increase the expression of antioxidant-related genes, including Gclc and Ec-sod, while reduce the transcription levels of oxidative-related genes, such as Rac1 and Nox4 demonstrated by both in vivo and in vitro studies. Further investigations found that Shenks could decrease the oxidative productions of protein (3-nitrotyrosine) and lipid (malondialdehyde) and increase GSH content both in bleomycin treated mouse lungs and TGF-β stimulated fibroblasts, as well as inhibit the production of ROS stimulated by TGF-β to fight against oxidative stress. Overall, Shenks inhibited fibrosis by blocking TGF-β pathway and modulating the oxidant/antioxidant balance.Haiyan ChuYing ShiShuai JiangQicheng ZhongYongqiang ZhaoQingmei LiuYanyun MaXiangguang ShiWeifeng DingXiaodong ZhouJimin CuiLi JinGang GuoJiucun WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haiyan Chu Ying Shi Shuai Jiang Qicheng Zhong Yongqiang Zhao Qingmei Liu Yanyun Ma Xiangguang Shi Weifeng Ding Xiaodong Zhou Jimin Cui Li Jin Gang Guo Jiucun Wang Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress
description	Abstract Pulmonary fibrosis is a kind of devastating interstitial lung disease due to the limited therapeutic strategies. Traditional Chinese medicine (TCM) practices have put forth Shenks as a promising treatment approach. Here, we performed in vivo study and in vitro study to delineate the anti-fibrotic mechanisms behind Shenks treatment for pulmonary fibrosis. We found that regardless of the prophylactic or therapeutic treatment, Shenks was able to attenuate BLM-induced-fibrosis in mice, down regulate extracellular matrix genes expression, and reduce collagen production. The aberrantly high Smad3 phosphorylation levels and SBE activity in TGF-β-induced fibroblasts were dramatically decreased as a result of Shenks treatment. At the same time, Shenks was able to increase the expression of antioxidant-related genes, including Gclc and Ec-sod, while reduce the transcription levels of oxidative-related genes, such as Rac1 and Nox4 demonstrated by both in vivo and in vitro studies. Further investigations found that Shenks could decrease the oxidative productions of protein (3-nitrotyrosine) and lipid (malondialdehyde) and increase GSH content both in bleomycin treated mouse lungs and TGF-β stimulated fibroblasts, as well as inhibit the production of ROS stimulated by TGF-β to fight against oxidative stress. Overall, Shenks inhibited fibrosis by blocking TGF-β pathway and modulating the oxidant/antioxidant balance.
format	article
author	Haiyan Chu Ying Shi Shuai Jiang Qicheng Zhong Yongqiang Zhao Qingmei Liu Yanyun Ma Xiangguang Shi Weifeng Ding Xiaodong Zhou Jimin Cui Li Jin Gang Guo Jiucun Wang
author_facet	Haiyan Chu Ying Shi Shuai Jiang Qicheng Zhong Yongqiang Zhao Qingmei Liu Yanyun Ma Xiangguang Shi Weifeng Ding Xiaodong Zhou Jimin Cui Li Jin Gang Guo Jiucun Wang
author_sort	Haiyan Chu
title	Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress
title_short	Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress
title_full	Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress
title_fullStr	Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress
title_full_unstemmed	Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress
title_sort	treatment effects of the traditional chinese medicine shenks in bleomycin-induced lung fibrosis through regulation of tgf-beta/smad3 signaling and oxidative stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0facbc85898245e5ac42ab18e616186d
work_keys_str_mv	AT haiyanchu treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT yingshi treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT shuaijiang treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT qichengzhong treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT yongqiangzhao treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT qingmeiliu treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT yanyunma treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT xiangguangshi treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT weifengding treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT xiaodongzhou treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT jimincui treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT lijin treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT gangguo treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress AT jiucunwang treatmenteffectsofthetraditionalchinesemedicineshenksinbleomycininducedlungfibrosisthroughregulationoftgfbetasmad3signalingandoxidativestress
_version_	1718395148458524672

Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress

Ejemplares similares