New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics

Abstract In this investigation, we report the effect on the microscopic dynamics and interactions of the cytokine interferon gamma (IFN-γ) and antibodies to IFN-γ (anti-IFN-γ) and to the interferon gamma receptor 1 (anti-IFNGR1) prepared in exceptionally dilute solutions of initial proteins. Using b...

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Autor principal:	Kristina N. Woods
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0fb7f4bc74944d049a0ba23b214bd7f3
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spelling	oai:doaj.org-article:0fb7f4bc74944d049a0ba23b214bd7f32021-12-02T16:31:47ZNew insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics10.1038/s41598-021-93326-12045-2322https://doaj.org/article/0fb7f4bc74944d049a0ba23b214bd7f32021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93326-1https://doaj.org/toc/2045-2322Abstract In this investigation, we report the effect on the microscopic dynamics and interactions of the cytokine interferon gamma (IFN-γ) and antibodies to IFN-γ (anti-IFN-γ) and to the interferon gamma receptor 1 (anti-IFNGR1) prepared in exceptionally dilute solutions of initial proteins. Using both THz spectroscopy and molecular dynamics simulations we have uncovered that the high dilution method of sample preparation results in the reorganization of the sample surface residue dynamics at the solvent–protein interface that leads to both structural and kinetic heterogeneous dynamics that ultimately create interactions that enhance the binding probability of the antigen binding site. Our results indicate that the modified interfacial dynamics of anti-IFN-γ and anti-IFGNR1 that we probe experimentally are directly associated with alterations in the complementarity regions of the distinct antibodies that designate both antigen–antibody affinity and recognition.Kristina N. WoodsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-26 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristina N. Woods New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
description	Abstract In this investigation, we report the effect on the microscopic dynamics and interactions of the cytokine interferon gamma (IFN-γ) and antibodies to IFN-γ (anti-IFN-γ) and to the interferon gamma receptor 1 (anti-IFNGR1) prepared in exceptionally dilute solutions of initial proteins. Using both THz spectroscopy and molecular dynamics simulations we have uncovered that the high dilution method of sample preparation results in the reorganization of the sample surface residue dynamics at the solvent–protein interface that leads to both structural and kinetic heterogeneous dynamics that ultimately create interactions that enhance the binding probability of the antigen binding site. Our results indicate that the modified interfacial dynamics of anti-IFN-γ and anti-IFGNR1 that we probe experimentally are directly associated with alterations in the complementarity regions of the distinct antibodies that designate both antigen–antibody affinity and recognition.
format	article
author	Kristina N. Woods
author_facet	Kristina N. Woods
author_sort	Kristina N. Woods
title	New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
title_short	New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
title_full	New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
title_fullStr	New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
title_full_unstemmed	New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
title_sort	new insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0fb7f4bc74944d049a0ba23b214bd7f3
work_keys_str_mv	AT kristinanwoods newinsightsintothemicroscopicinteractionsassociatedwiththephysicalmechanismofactionofhighlydilutedbiologics
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New insights into the microscopic interactions associated with the physical mechanism of action of highly diluted biologics

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