Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification

Yuemei Han,1,* Junmei Tang,1,* Jiayi Xia,1 Rui Wang,1 Chen Qin,1 Sihao Liu,1 Xia Zhao,1 Hao Chen,1,2 Quankui Lin1,2 1School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, People’s Republic of China; 2Wenzhou Institute of Biomaterials and Eng...

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Autores principales:	Han Y, Tang J, Xia J, Wang R, Qin C, Liu S, Zhao X, Chen H, Lin Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	surface modification intraocular lens posterior capsular opacification drug eluting coating nanoparticle Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0fbe8a61ffc04eb7acdebacbb53d44e9
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id	oai:doaj.org-article:0fbe8a61ffc04eb7acdebacbb53d44e9
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	surface modification intraocular lens posterior capsular opacification drug eluting coating nanoparticle Medicine (General) R5-920
spellingShingle	surface modification intraocular lens posterior capsular opacification drug eluting coating nanoparticle Medicine (General) R5-920 Han Y Tang J Xia J Wang R Qin C Liu S Zhao X Chen H Lin Q Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification
description	Yuemei Han,1,* Junmei Tang,1,* Jiayi Xia,1 Rui Wang,1 Chen Qin,1 Sihao Liu,1 Xia Zhao,1 Hao Chen,1,2 Quankui Lin1,2 1School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, People’s Republic of China; 2Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao ChenSchool of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, 270 Xueyuan Xi Road, Wenzhou 325027, People’s Republic of ChinaTel/Fax +86 577 8806 7962Email chenhao823@mail.eye.ac.cnQuankui LinSchool of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, 270 Xueyuan Xi Road, Wenzhou 325027, People’s Republic of ChinaTel/Fax +86 577 8806 7962Email linqk@wmu.edu.cnBackground: Posterior capsular opacification (PCO) is the main complication after intraocular lens (IOL) implantation in cataract surgery, which is the result of lens epithelial cell (LEC) adhesion, proliferation and migration on the IOL and at the lens capsule interface. Hydrophilic surface modification, such as surface heparinization, decreases the cell adhesion, which has been commercialized and used clinically. However, clinical long-term observation results show no significant difference between the pristine and heparinized IOLs.Methods: To prevent PCO over the long time span, we modified the IOLs with an antiproliferative drug-loaded hydrophilic coating. The antiproliferative drug doxorubicin (DOX)-incorporated chitosan (CHI) nanoparticle was fabricated by sodium tripolyphosphate (TPP) gelation. Such antiproliferative drug-loaded CHI-TPP-DOX nanoparticles (CTDNP) were used as one of the building blocks to prepare polyelectrolyte multilayer with heparin (HEP) via layer-by-layer assembly, obtaining (HEP/CTDNP)n multilayers. The assembly process was characterized by quartz crystal microbalance with dissipation (QCM-D). The drug release behavior of the coating was investigated by ultra-HPLC (UPLC). In vitro cell experiments were carried out to monitor the effects of multifunctional coatings on cellular adhesion, proliferation and migration. And the intraocular implantation was performed on rabbits to evaluate the in vivo PCO inhibitory effect of such surface-functionalized IOLs.Results: The positively charged CTDNP was successfully prepared by ionic gelation. The QCM-D results indicate the successful preparation of the (HEP/CTDNP)n multilayer film. Drug release profiles showed that surface-multifunctionalized IOL had drug-sustained release properties. In vitro cell culture results showed significant inhibition of adhesion, proliferation and migration of LECs after surface modification. The in vivo results showed that the IOLs with multifunctionalized surface can effectively reduce the posterior hyperplasia and Soemmering’s ring (SR) formation.Conclusion: These findings suggested that such multifunctionalized drug-eluting IOLs can effectively reduce the posterior hyperplasia and SR formation when intraocular implantation has a major impact on reducing PCO incidence. Thus they have a great potential in improving patient vision recovery and maintenance.Keywords: surface modification, intraocular lens, posterior capsular opacification, drug-eluting coating, nanoparticle
format	article
author	Han Y Tang J Xia J Wang R Qin C Liu S Zhao X Chen H Lin Q
author_facet	Han Y Tang J Xia J Wang R Qin C Liu S Zhao X Chen H Lin Q
author_sort	Han Y
title	Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification
title_short	Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification
title_full	Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification
title_fullStr	Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification
title_full_unstemmed	Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification
title_sort	anti-adhesive and antiproliferative synergistic surface modification of intraocular lens for reduced posterior capsular opacification
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/0fbe8a61ffc04eb7acdebacbb53d44e9
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spelling	oai:doaj.org-article:0fbe8a61ffc04eb7acdebacbb53d44e92021-12-02T05:45:16ZAnti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification1178-2013https://doaj.org/article/0fbe8a61ffc04eb7acdebacbb53d44e92019-11-01T00:00:00Zhttps://www.dovepress.com/anti-adhesive-and-antiproliferative-synergistic-surface-modification-o-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yuemei Han,1,* Junmei Tang,1,* Jiayi Xia,1 Rui Wang,1 Chen Qin,1 Sihao Liu,1 Xia Zhao,1 Hao Chen,1,2 Quankui Lin1,2 1School of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, People’s Republic of China; 2Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou 32500, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hao ChenSchool of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, 270 Xueyuan Xi Road, Wenzhou 325027, People’s Republic of ChinaTel/Fax +86 577 8806 7962Email chenhao823@mail.eye.ac.cnQuankui LinSchool of Ophthalmology & Optometry, Eye Hospital, Wenzhou Medical University, 270 Xueyuan Xi Road, Wenzhou 325027, People’s Republic of ChinaTel/Fax +86 577 8806 7962Email linqk@wmu.edu.cnBackground: Posterior capsular opacification (PCO) is the main complication after intraocular lens (IOL) implantation in cataract surgery, which is the result of lens epithelial cell (LEC) adhesion, proliferation and migration on the IOL and at the lens capsule interface. Hydrophilic surface modification, such as surface heparinization, decreases the cell adhesion, which has been commercialized and used clinically. However, clinical long-term observation results show no significant difference between the pristine and heparinized IOLs.Methods: To prevent PCO over the long time span, we modified the IOLs with an antiproliferative drug-loaded hydrophilic coating. The antiproliferative drug doxorubicin (DOX)-incorporated chitosan (CHI) nanoparticle was fabricated by sodium tripolyphosphate (TPP) gelation. Such antiproliferative drug-loaded CHI-TPP-DOX nanoparticles (CTDNP) were used as one of the building blocks to prepare polyelectrolyte multilayer with heparin (HEP) via layer-by-layer assembly, obtaining (HEP/CTDNP)n multilayers. The assembly process was characterized by quartz crystal microbalance with dissipation (QCM-D). The drug release behavior of the coating was investigated by ultra-HPLC (UPLC). In vitro cell experiments were carried out to monitor the effects of multifunctional coatings on cellular adhesion, proliferation and migration. And the intraocular implantation was performed on rabbits to evaluate the in vivo PCO inhibitory effect of such surface-functionalized IOLs.Results: The positively charged CTDNP was successfully prepared by ionic gelation. The QCM-D results indicate the successful preparation of the (HEP/CTDNP)n multilayer film. Drug release profiles showed that surface-multifunctionalized IOL had drug-sustained release properties. In vitro cell culture results showed significant inhibition of adhesion, proliferation and migration of LECs after surface modification. The in vivo results showed that the IOLs with multifunctionalized surface can effectively reduce the posterior hyperplasia and Soemmering’s ring (SR) formation.Conclusion: These findings suggested that such multifunctionalized drug-eluting IOLs can effectively reduce the posterior hyperplasia and SR formation when intraocular implantation has a major impact on reducing PCO incidence. Thus they have a great potential in improving patient vision recovery and maintenance.Keywords: surface modification, intraocular lens, posterior capsular opacification, drug-eluting coating, nanoparticle    Han YTang JXia JWang RQin CLiu SZhao XChen HLin QDove Medical Pressarticlesurface modificationintraocular lensposterior capsular opacificationdrug eluting coatingnanoparticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9047-9061 (2019)

Anti-Adhesive And Antiproliferative Synergistic Surface Modification Of Intraocular Lens For Reduced Posterior Capsular Opacification

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