Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.

Chemoresistance develops in triple negative breast cancer cells during prolonged therapy with chemotherapeutic drugs, diminishing the benefits of these drugs in breast cancer patients. Palmatine, a natural alkaloid extracted from several medicinal plants in West Africa, has been studied in vitro and...

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Autores principales:	Selase Ativui, Cynthia A. Danquah, Newman Osafo, Williams Adu, Michael Ofori
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Chemoresistance Palmatine Triple negative breast cancer multi drug resistant protein Science Q
Acceso en línea:	https://doaj.org/article/0fc6869174134688b6db0ef96680d04a
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spelling	oai:doaj.org-article:0fc6869174134688b6db0ef96680d04a2021-11-22T04:28:05ZPalmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.2468-227610.1016/j.sciaf.2021.e01022https://doaj.org/article/0fc6869174134688b6db0ef96680d04a2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468227621003239https://doaj.org/toc/2468-2276Chemoresistance develops in triple negative breast cancer cells during prolonged therapy with chemotherapeutic drugs, diminishing the benefits of these drugs in breast cancer patients. Palmatine, a natural alkaloid extracted from several medicinal plants in West Africa, has been studied in vitro and found to be a promising anticancer agent for treating triple negative breast cancer. This study seeks to investigate the therapeutic potential of palmatine in sensitizing resistant 4T1 triple negative breast cancer cells to doxorubicin therapy. Cell viability, Hoechst dye membrane transport, intracellular doxorubicin accumulation and Adenosine triphosphatase (ATPase) assays were performed. From the results, the intracellular concentrations of the Hoechst dye and doxorubicin were substantially increased following treatment with palmatine. In addition, palmatine inhibited the doxorubicin efflux activity of the Multidrug resistance protein 1 (MDR1) by stimulating the activity of the ATPase enzymes. A combination of low concentrations of palmatine and verapamil best achieved these results. This research highlights the use of palmatine as a chemosensitizer to aid the treatment of triple negative breast cancer disease.Selase AtivuiCynthia A. DanquahNewman OsafoWilliams AduMichael OforiElsevierarticleChemoresistancePalmatineTriple negative breast cancermulti drug resistant proteinScienceQENScientific African, Vol 14, Iss , Pp e01022- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chemoresistance Palmatine Triple negative breast cancer multi drug resistant protein Science Q
spellingShingle	Chemoresistance Palmatine Triple negative breast cancer multi drug resistant protein Science Q Selase Ativui Cynthia A. Danquah Newman Osafo Williams Adu Michael Ofori Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
description	Chemoresistance develops in triple negative breast cancer cells during prolonged therapy with chemotherapeutic drugs, diminishing the benefits of these drugs in breast cancer patients. Palmatine, a natural alkaloid extracted from several medicinal plants in West Africa, has been studied in vitro and found to be a promising anticancer agent for treating triple negative breast cancer. This study seeks to investigate the therapeutic potential of palmatine in sensitizing resistant 4T1 triple negative breast cancer cells to doxorubicin therapy. Cell viability, Hoechst dye membrane transport, intracellular doxorubicin accumulation and Adenosine triphosphatase (ATPase) assays were performed. From the results, the intracellular concentrations of the Hoechst dye and doxorubicin were substantially increased following treatment with palmatine. In addition, palmatine inhibited the doxorubicin efflux activity of the Multidrug resistance protein 1 (MDR1) by stimulating the activity of the ATPase enzymes. A combination of low concentrations of palmatine and verapamil best achieved these results. This research highlights the use of palmatine as a chemosensitizer to aid the treatment of triple negative breast cancer disease.
format	article
author	Selase Ativui Cynthia A. Danquah Newman Osafo Williams Adu Michael Ofori
author_facet	Selase Ativui Cynthia A. Danquah Newman Osafo Williams Adu Michael Ofori
author_sort	Selase Ativui
title	Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
title_short	Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
title_full	Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
title_fullStr	Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
title_full_unstemmed	Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
title_sort	palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/0fc6869174134688b6db0ef96680d04a
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Palmatine sensitizes chemoresistant triple negative breast cancer cells via efflux inhibition of multidrug resistant protein 1.

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