Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing

Abstract Severe water deficit and highly polluting effluent generation from leather industries have constantly been pressurizing the tanners to adopt cleaner leather processing systems. The present study aims to minimize the use of water by substituting it with non-aqueous green solvents and also to...

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Autores principales:	Poornima Ramamoorthi, Aravindhan Rathinam, Raghava Rao Jonnalagadda, Thanikaivelan Palanisamy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0fca24a7d3ac47d4b3d6910d548fb263
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spelling	oai:doaj.org-article:0fca24a7d3ac47d4b3d6910d548fb2632021-12-02T13:34:11ZNon-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing10.1038/s41598-020-79406-82045-2322https://doaj.org/article/0fca24a7d3ac47d4b3d6910d548fb2632020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79406-8https://doaj.org/toc/2045-2322Abstract Severe water deficit and highly polluting effluent generation from leather industries have constantly been pressurizing the tanners to adopt cleaner leather processing systems. The present study aims to minimize the use of water by substituting it with non-aqueous green solvents and also to enhance the enzyme action in alpha-amylase based fiber opening process. The activity of alpha-amylase in select non-aqueous green solvents namely, heptane, polyethylene glycol 200 and propylene glycol is considerably higher by 62, 38 and 31% than in water, respectively. Comparable results are obtained for the catalytic efficiency of alpha-amylase and hence it is further validated in collagen fiber opening trials as well. Scanning electron micrographs, histological images and proteoglycan estimation supported the above findings at 1% alpha-amylase dosage. The final quality of the experimental leathers in terms of physical and bulk properties is comparable to that of control leathers. Recycling studies indicate that it is possible to replace water with green solvents for enzymatic fiber opening with the feasibility to recover more than 85% solvent-enzyme mixture and reuse without any additional alpha-amylase usage. Reduction in pollution load coupled with the efficient catalytic action of enzyme in non-aqueous media favors the present protocol for industrial applications.Poornima RamamoorthiAravindhan RathinamRaghava Rao JonnalagaddaThanikaivelan PalanisamyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Poornima Ramamoorthi Aravindhan Rathinam Raghava Rao Jonnalagadda Thanikaivelan Palanisamy Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
description	Abstract Severe water deficit and highly polluting effluent generation from leather industries have constantly been pressurizing the tanners to adopt cleaner leather processing systems. The present study aims to minimize the use of water by substituting it with non-aqueous green solvents and also to enhance the enzyme action in alpha-amylase based fiber opening process. The activity of alpha-amylase in select non-aqueous green solvents namely, heptane, polyethylene glycol 200 and propylene glycol is considerably higher by 62, 38 and 31% than in water, respectively. Comparable results are obtained for the catalytic efficiency of alpha-amylase and hence it is further validated in collagen fiber opening trials as well. Scanning electron micrographs, histological images and proteoglycan estimation supported the above findings at 1% alpha-amylase dosage. The final quality of the experimental leathers in terms of physical and bulk properties is comparable to that of control leathers. Recycling studies indicate that it is possible to replace water with green solvents for enzymatic fiber opening with the feasibility to recover more than 85% solvent-enzyme mixture and reuse without any additional alpha-amylase usage. Reduction in pollution load coupled with the efficient catalytic action of enzyme in non-aqueous media favors the present protocol for industrial applications.
format	article
author	Poornima Ramamoorthi Aravindhan Rathinam Raghava Rao Jonnalagadda Thanikaivelan Palanisamy
author_facet	Poornima Ramamoorthi Aravindhan Rathinam Raghava Rao Jonnalagadda Thanikaivelan Palanisamy
author_sort	Poornima Ramamoorthi
title	Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
title_short	Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
title_full	Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
title_fullStr	Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
title_full_unstemmed	Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
title_sort	non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0fca24a7d3ac47d4b3d6910d548fb263
work_keys_str_mv	AT poornimaramamoorthi nonaqueousgreensolventsimprovealphaamylaseinducedfiberopeninginleatherprocessing AT aravindhanrathinam nonaqueousgreensolventsimprovealphaamylaseinducedfiberopeninginleatherprocessing AT raghavaraojonnalagadda nonaqueousgreensolventsimprovealphaamylaseinducedfiberopeninginleatherprocessing AT thanikaivelanpalanisamy nonaqueousgreensolventsimprovealphaamylaseinducedfiberopeninginleatherprocessing
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Non-aqueous green solvents improve alpha-amylase induced fiber opening in leather processing

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