Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

Ana Carolina Mazarin de Moraes,1 Bruna Araujo Lima,2 Andreia Fonseca de Faria,1 Marcelo Brocchi,2 Oswaldo Luiz Alves1 1Laboratory of Solid State Chemistry, Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil; 2Department of Genetics, Evolution and Bioagents, Insti...

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Autores principales:	de Moraes ACM, Lima BA, de Faria AF, Brocchi M, Alves OL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/0fca847796af4db7835e4eef640dea76
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spelling	oai:doaj.org-article:0fca847796af4db7835e4eef640dea762021-12-02T07:44:03ZGraphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus1178-2013https://doaj.org/article/0fca847796af4db7835e4eef640dea762015-11-01T00:00:00Zhttps://www.dovepress.com/graphene-oxide-silver-nanocomposite-as-a-promising-biocidal-agent-agai-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ana Carolina Mazarin de Moraes,1 Bruna Araujo Lima,2 Andreia Fonseca de Faria,1 Marcelo Brocchi,2 Oswaldo Luiz Alves1 1Laboratory of Solid State Chemistry, Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil; 2Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil Background: Methicillin-resistant Staphylococcus aureus (MRSA) has been responsible for serious hospital infections worldwide. Nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. In the past decade, graphene oxide (GO) has emerged as a material that is often used to support and stabilize silver nanoparticles (AgNPs) for the preparation of novel antibacterial nanocomposites. In this work, we report the synthesis of the graphene-oxide silver nanocomposite (GO-Ag) and its antibacterial activity against relevant microorganisms in medicine. Materials and methods: GO-Ag nanocomposite was synthesized through the reduction of silver ions (Ag+) by sodium citrate in an aqueous GO dispersion, and was extensively characterized using ultraviolet-visible absorption spectroscopy, X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, and transmission electron microscopy. The antibacterial activity was evaluated by microdilution assays and time-kill experiments. The morphology of bacterial cells treated with GO-Ag was investigated via transmission electron microscopy. Results: AgNPs were well distributed throughout GO sheets, with an average size of 9.4±2.8 nm. The GO-Ag nanocomposite exhibited an excellent antibacterial activity against methicillin-resistant S. aureus, Acinetobacter baumannii, Enterococcus faecalis, and Escherichia coli. All (100%) MRSA cells were inactivated after 4 hours of exposure to GO-Ag sheets. In addition, no toxicity was found for either pristine GO or bare AgNPs within the tested concentration range. Transmission electronic microscopy images offered insights into how GO-Ag nanosheets interacted with bacterial cells. Conclusion: Our results indicate that the GO-Ag nanocomposite is a promising antibacterial agent against common nosocomial bacteria, particularly antibiotic-resistant MRSA. Morphological injuries on MRSA cells revealed a likely loss of viability as a result of the direct contact between bacteria and the GO-Ag sheets. Keywords: graphene oxide, silver nanoparticles, graphene oxide-silver nanocomposite, antibacterial agent, MRSA, Escherichia colide Moraes ACMLima BAde Faria AFBrocchi MAlves OLDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6847-6861 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 de Moraes ACM Lima BA de Faria AF Brocchi M Alves OL Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus
description	Ana Carolina Mazarin de Moraes,1 Bruna Araujo Lima,2 Andreia Fonseca de Faria,1 Marcelo Brocchi,2 Oswaldo Luiz Alves1 1Laboratory of Solid State Chemistry, Institute of Chemistry, University of Campinas, Campinas, São Paulo, Brazil; 2Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil Background: Methicillin-resistant Staphylococcus aureus (MRSA) has been responsible for serious hospital infections worldwide. Nanomaterials are an alternative to conventional antibiotic compounds, because bacteria are unlikely to develop microbial resistance against nanomaterials. In the past decade, graphene oxide (GO) has emerged as a material that is often used to support and stabilize silver nanoparticles (AgNPs) for the preparation of novel antibacterial nanocomposites. In this work, we report the synthesis of the graphene-oxide silver nanocomposite (GO-Ag) and its antibacterial activity against relevant microorganisms in medicine. Materials and methods: GO-Ag nanocomposite was synthesized through the reduction of silver ions (Ag+) by sodium citrate in an aqueous GO dispersion, and was extensively characterized using ultraviolet-visible absorption spectroscopy, X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, and transmission electron microscopy. The antibacterial activity was evaluated by microdilution assays and time-kill experiments. The morphology of bacterial cells treated with GO-Ag was investigated via transmission electron microscopy. Results: AgNPs were well distributed throughout GO sheets, with an average size of 9.4±2.8 nm. The GO-Ag nanocomposite exhibited an excellent antibacterial activity against methicillin-resistant S. aureus, Acinetobacter baumannii, Enterococcus faecalis, and Escherichia coli. All (100%) MRSA cells were inactivated after 4 hours of exposure to GO-Ag sheets. In addition, no toxicity was found for either pristine GO or bare AgNPs within the tested concentration range. Transmission electronic microscopy images offered insights into how GO-Ag nanosheets interacted with bacterial cells. Conclusion: Our results indicate that the GO-Ag nanocomposite is a promising antibacterial agent against common nosocomial bacteria, particularly antibiotic-resistant MRSA. Morphological injuries on MRSA cells revealed a likely loss of viability as a result of the direct contact between bacteria and the GO-Ag sheets. Keywords: graphene oxide, silver nanoparticles, graphene oxide-silver nanocomposite, antibacterial agent, MRSA, Escherichia coli
format	article
author	de Moraes ACM Lima BA de Faria AF Brocchi M Alves OL
author_facet	de Moraes ACM Lima BA de Faria AF Brocchi M Alves OL
author_sort	de Moraes ACM
title	Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus
title_short	Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus
title_full	Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus
title_fullStr	Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus
title_full_unstemmed	Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus
title_sort	graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant staphylococcus aureus
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/0fca847796af4db7835e4eef640dea76
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Graphene oxide-silver nanocomposite as a promising biocidal agent against methicillin-resistant Staphylococcus aureus

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