psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation

ATP-dependent helicases remodel the spliceosome and proofread splice site recognition. A new method – Purified Spliceosome iCLIP (psiCLIP) – probes protein-RNA interactions in defined spliceosome complexes to reveal how the helicases Prp16 and Prp22 promote correct mRNA synthesis through dynamic bin...

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Autores principales:	Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule, Kiyoshi Nagai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0fcc6541964d40bfa8b86f4263c781c4
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spelling	oai:doaj.org-article:0fcc6541964d40bfa8b86f4263c781c42021-12-02T15:52:50ZpsiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation10.1038/s41467-021-21745-92041-1723https://doaj.org/article/0fcc6541964d40bfa8b86f4263c781c42021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21745-9https://doaj.org/toc/2041-1723ATP-dependent helicases remodel the spliceosome and proofread splice site recognition. A new method – Purified Spliceosome iCLIP (psiCLIP) – probes protein-RNA interactions in defined spliceosome complexes to reveal how the helicases Prp16 and Prp22 promote correct mRNA synthesis through dynamic binding on their RNA substrates.Lisa M. StrittmatterCharlotte CapitanchikAndrew J. NewmanMartina HalleggerChristine M. NormanSebastian M. FicaChris OubridgeNicholas M. LuscombeJernej UleKiyoshi NagaiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Lisa M. Strittmatter Charlotte Capitanchik Andrew J. Newman Martina Hallegger Christine M. Norman Sebastian M. Fica Chris Oubridge Nicholas M. Luscombe Jernej Ule Kiyoshi Nagai psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
description	ATP-dependent helicases remodel the spliceosome and proofread splice site recognition. A new method – Purified Spliceosome iCLIP (psiCLIP) – probes protein-RNA interactions in defined spliceosome complexes to reveal how the helicases Prp16 and Prp22 promote correct mRNA synthesis through dynamic binding on their RNA substrates.
format	article
author	Lisa M. Strittmatter Charlotte Capitanchik Andrew J. Newman Martina Hallegger Christine M. Norman Sebastian M. Fica Chris Oubridge Nicholas M. Luscombe Jernej Ule Kiyoshi Nagai
author_facet	Lisa M. Strittmatter Charlotte Capitanchik Andrew J. Newman Martina Hallegger Christine M. Norman Sebastian M. Fica Chris Oubridge Nicholas M. Luscombe Jernej Ule Kiyoshi Nagai
author_sort	Lisa M. Strittmatter
title	psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
title_short	psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
title_full	psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
title_fullStr	psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
title_full_unstemmed	psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation
title_sort	psiclip reveals dynamic rna binding by deah-box helicases before and after exon ligation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0fcc6541964d40bfa8b86f4263c781c4
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psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation

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