Biocompatibility of a droplet-mixing device using an electro-conjugate fluid

An electro-conjugate fluid (ECF) is a type of functional fluid that produces a jet flow under a non-uniform electric field. We use an ECF to generate a rotational flow in a tiny cylindrical chamber. When multiple droplets are dripped into the rotational flow of the ECF, they may be subjected to a ce...

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Autores principales:	Kenjiro TAKEMURA, Kiho KOBAYASHI, Kazuya EDAMURA
Formato:	article
Lenguaje:	EN
Publicado:	The Japan Society of Mechanical Engineers 2016
Materias:	μtas mixing electro-conjugate fluid functional fluid bioluminescence Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/0fd628d6dc034a849a3f7de46b33ac53
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spelling	oai:doaj.org-article:0fd628d6dc034a849a3f7de46b33ac532021-11-26T06:40:17ZBiocompatibility of a droplet-mixing device using an electro-conjugate fluid2187-974510.1299/mej.15-00421https://doaj.org/article/0fd628d6dc034a849a3f7de46b33ac532016-02-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/mej/3/2/3_15-00421/_pdf/-char/enhttps://doaj.org/toc/2187-9745An electro-conjugate fluid (ECF) is a type of functional fluid that produces a jet flow under a non-uniform electric field. We use an ECF to generate a rotational flow in a tiny cylindrical chamber. When multiple droplets are dripped into the rotational flow of the ECF, they may be subjected to a centripetal force because of the difference in density between the droplets and ECF. This causes the droplets to move to the center of the chamber and mix with each other. In this paper, we conducted luminance measurements using a bioluminescence reaction to verify the suitability of this device for biological applications. We observed the luminescence produced by mixing droplets of luciferin and luciferase, and measured luminance maxima and area to assess the mixing ability of the device. In addition, because the enzyme luciferase can be deactivated by surroundings, we confirmed whether the proposed mixing method has any harmful influence on biological samples or not. To better understand the properties of the device, we conducted control experiments with two other set of conditions; i.e., without ECF and with still ECF. Our results confirmed that there was no difference in mixing ability between the three systems, and the influence of an electric- and centrifugal-field generated in the device on mixing might be negligible for biological applications.Kenjiro TAKEMURAKiho KOBAYASHIKazuya EDAMURAThe Japan Society of Mechanical Engineersarticleμtasmixingelectro-conjugate fluidfunctional fluidbioluminescenceMechanical engineering and machineryTJ1-1570ENMechanical Engineering Journal, Vol 3, Iss 2, Pp 15-00421-15-00421 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	μtas mixing electro-conjugate fluid functional fluid bioluminescence Mechanical engineering and machinery TJ1-1570
spellingShingle	μtas mixing electro-conjugate fluid functional fluid bioluminescence Mechanical engineering and machinery TJ1-1570 Kenjiro TAKEMURA Kiho KOBAYASHI Kazuya EDAMURA Biocompatibility of a droplet-mixing device using an electro-conjugate fluid
description	An electro-conjugate fluid (ECF) is a type of functional fluid that produces a jet flow under a non-uniform electric field. We use an ECF to generate a rotational flow in a tiny cylindrical chamber. When multiple droplets are dripped into the rotational flow of the ECF, they may be subjected to a centripetal force because of the difference in density between the droplets and ECF. This causes the droplets to move to the center of the chamber and mix with each other. In this paper, we conducted luminance measurements using a bioluminescence reaction to verify the suitability of this device for biological applications. We observed the luminescence produced by mixing droplets of luciferin and luciferase, and measured luminance maxima and area to assess the mixing ability of the device. In addition, because the enzyme luciferase can be deactivated by surroundings, we confirmed whether the proposed mixing method has any harmful influence on biological samples or not. To better understand the properties of the device, we conducted control experiments with two other set of conditions; i.e., without ECF and with still ECF. Our results confirmed that there was no difference in mixing ability between the three systems, and the influence of an electric- and centrifugal-field generated in the device on mixing might be negligible for biological applications.
format	article
author	Kenjiro TAKEMURA Kiho KOBAYASHI Kazuya EDAMURA
author_facet	Kenjiro TAKEMURA Kiho KOBAYASHI Kazuya EDAMURA
author_sort	Kenjiro TAKEMURA
title	Biocompatibility of a droplet-mixing device using an electro-conjugate fluid
title_short	Biocompatibility of a droplet-mixing device using an electro-conjugate fluid
title_full	Biocompatibility of a droplet-mixing device using an electro-conjugate fluid
title_fullStr	Biocompatibility of a droplet-mixing device using an electro-conjugate fluid
title_full_unstemmed	Biocompatibility of a droplet-mixing device using an electro-conjugate fluid
title_sort	biocompatibility of a droplet-mixing device using an electro-conjugate fluid
publisher	The Japan Society of Mechanical Engineers
publishDate	2016
url	https://doaj.org/article/0fd628d6dc034a849a3f7de46b33ac53
work_keys_str_mv	AT kenjirotakemura biocompatibilityofadropletmixingdeviceusinganelectroconjugatefluid AT kihokobayashi biocompatibilityofadropletmixingdeviceusinganelectroconjugatefluid AT kazuyaedamura biocompatibilityofadropletmixingdeviceusinganelectroconjugatefluid
_version_	1718409701382684672

Biocompatibility of a droplet-mixing device using an electro-conjugate fluid

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