Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

Abstract Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luc...

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Autores principales:	Ryo Nishihara, Masahiro Abe, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0fd91365332d49d7b0d8b82a604a2b2e
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spelling	oai:doaj.org-article:0fd91365332d49d7b0d8b82a604a2b2e2021-12-02T15:05:13ZLuciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays10.1038/s41598-017-00955-62045-2322https://doaj.org/article/0fd91365332d49d7b0d8b82a604a2b2e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00955-6https://doaj.org/toc/2045-2322Abstract Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.Ryo NishiharaMasahiro AbeShigeru NishiyamaDaniel CitterioKoji SuzukiSung Bae KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryo Nishihara Masahiro Abe Shigeru Nishiyama Daniel Citterio Koji Suzuki Sung Bae Kim Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
description	Abstract Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.
format	article
author	Ryo Nishihara Masahiro Abe Shigeru Nishiyama Daniel Citterio Koji Suzuki Sung Bae Kim
author_facet	Ryo Nishihara Masahiro Abe Shigeru Nishiyama Daniel Citterio Koji Suzuki Sung Bae Kim
author_sort	Ryo Nishihara
title	Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
title_short	Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
title_full	Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
title_fullStr	Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
title_full_unstemmed	Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
title_sort	luciferase-specific coelenterazine analogues for optical contamination-free bioassays
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0fd91365332d49d7b0d8b82a604a2b2e
work_keys_str_mv	AT ryonishihara luciferasespecificcoelenterazineanaloguesforopticalcontaminationfreebioassays AT masahiroabe luciferasespecificcoelenterazineanaloguesforopticalcontaminationfreebioassays AT shigerunishiyama luciferasespecificcoelenterazineanaloguesforopticalcontaminationfreebioassays AT danielcitterio luciferasespecificcoelenterazineanaloguesforopticalcontaminationfreebioassays AT kojisuzuki luciferasespecificcoelenterazineanaloguesforopticalcontaminationfreebioassays AT sungbaekim luciferasespecificcoelenterazineanaloguesforopticalcontaminationfreebioassays
_version_	1718388874144645120

Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

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