Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

Abstract Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery be...

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Autores principales:	David J. Quispe-Parra, Joshua G. Medina-Feliciano, Sebastián Cruz-González, Humberto Ortiz-Zuazaga, José E. García-Arrarás
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0fda7bc74cc544f3b82fe4689d1b5016
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spelling	oai:doaj.org-article:0fda7bc74cc544f3b82fe4689d1b50162021-12-02T15:23:02ZTranscriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima10.1038/s41598-020-79436-22045-2322https://doaj.org/article/0fda7bc74cc544f3b82fe4689d1b50162021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79436-2https://doaj.org/toc/2045-2322Abstract Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery behind this faculty, however, remains to be understood. Here we assembled and annotated a de novo transcriptome using RNA-seq data consisting of regenerating and non-regenerating intestinal tissues from the sea cucumber Holothuria glaberrima. Comparisons of differential expression were made using the mesentery as a reference against 24 h and 3 days regenerating intestine, revealing a large number of differentially expressed transcripts. Gene ontology and pathway enrichment analysis showed evidence of increasing transcriptional activity. Further analysis of transcripts associated with transcription factors revealed diverse expression patterns with mechanisms involving developmental and cancer-related activity that could be related to the regenerative process. Our study demonstrates the broad and diversified gene expression profile during the early stages of the process using the mesentery as the focal point of intestinal regeneration. It also establishes the genes that are the most important candidates in the cellular processes that underlie regenerative responses.David J. Quispe-ParraJoshua G. Medina-FelicianoSebastián Cruz-GonzálezHumberto Ortiz-ZuazagaJosé E. García-ArrarásNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David J. Quispe-Parra Joshua G. Medina-Feliciano Sebastián Cruz-González Humberto Ortiz-Zuazaga José E. García-Arrarás Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima
description	Abstract Echinoderms comprise a group of animals with impressive regenerative capabilities. They can replace complex internal organs following injury or autotomy. In holothurians or sea cucumbers, cellular processes of intestinal regeneration have been extensively studied. The molecular machinery behind this faculty, however, remains to be understood. Here we assembled and annotated a de novo transcriptome using RNA-seq data consisting of regenerating and non-regenerating intestinal tissues from the sea cucumber Holothuria glaberrima. Comparisons of differential expression were made using the mesentery as a reference against 24 h and 3 days regenerating intestine, revealing a large number of differentially expressed transcripts. Gene ontology and pathway enrichment analysis showed evidence of increasing transcriptional activity. Further analysis of transcripts associated with transcription factors revealed diverse expression patterns with mechanisms involving developmental and cancer-related activity that could be related to the regenerative process. Our study demonstrates the broad and diversified gene expression profile during the early stages of the process using the mesentery as the focal point of intestinal regeneration. It also establishes the genes that are the most important candidates in the cellular processes that underlie regenerative responses.
format	article
author	David J. Quispe-Parra Joshua G. Medina-Feliciano Sebastián Cruz-González Humberto Ortiz-Zuazaga José E. García-Arrarás
author_facet	David J. Quispe-Parra Joshua G. Medina-Feliciano Sebastián Cruz-González Humberto Ortiz-Zuazaga José E. García-Arrarás
author_sort	David J. Quispe-Parra
title	Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima
title_short	Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima
title_full	Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima
title_fullStr	Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima
title_full_unstemmed	Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima
title_sort	transcriptomic analysis of early stages of intestinal regeneration in holothuria glaberrima
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0fda7bc74cc544f3b82fe4689d1b5016
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Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima

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