Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts

The early steps of ribosome assembly occur co-transcriptionally on the nascent ribosomal RNA. Here the authors demonstrate an approach that allows simultaneous monitoring of transcription and ribosomal protein assembly at the single-molecule level in real time.

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Autores principales: Olivier Duss, Galina A. Stepanyuk, Annette Grot, Seán E. O’Leary, Joseph D. Puglisi, James R. Williamson
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/0fe00cc7c750481fa2a7c9346d042e43
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spelling oai:doaj.org-article:0fe00cc7c750481fa2a7c9346d042e432021-12-02T15:34:40ZReal-time assembly of ribonucleoprotein complexes on nascent RNA transcripts10.1038/s41467-018-07423-32041-1723https://doaj.org/article/0fe00cc7c750481fa2a7c9346d042e432018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07423-3https://doaj.org/toc/2041-1723The early steps of ribosome assembly occur co-transcriptionally on the nascent ribosomal RNA. Here the authors demonstrate an approach that allows simultaneous monitoring of transcription and ribosomal protein assembly at the single-molecule level in real time.Olivier DussGalina A. StepanyukAnnette GrotSeán E. O’LearyJoseph D. PuglisiJames R. WilliamsonNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Olivier Duss
Galina A. Stepanyuk
Annette Grot
Seán E. O’Leary
Joseph D. Puglisi
James R. Williamson
Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
description The early steps of ribosome assembly occur co-transcriptionally on the nascent ribosomal RNA. Here the authors demonstrate an approach that allows simultaneous monitoring of transcription and ribosomal protein assembly at the single-molecule level in real time.
format article
author Olivier Duss
Galina A. Stepanyuk
Annette Grot
Seán E. O’Leary
Joseph D. Puglisi
James R. Williamson
author_facet Olivier Duss
Galina A. Stepanyuk
Annette Grot
Seán E. O’Leary
Joseph D. Puglisi
James R. Williamson
author_sort Olivier Duss
title Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
title_short Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
title_full Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
title_fullStr Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
title_full_unstemmed Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts
title_sort real-time assembly of ribonucleoprotein complexes on nascent rna transcripts
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/0fe00cc7c750481fa2a7c9346d042e43
work_keys_str_mv AT olivierduss realtimeassemblyofribonucleoproteincomplexesonnascentrnatranscripts
AT galinaastepanyuk realtimeassemblyofribonucleoproteincomplexesonnascentrnatranscripts
AT annettegrot realtimeassemblyofribonucleoproteincomplexesonnascentrnatranscripts
AT seaneoleary realtimeassemblyofribonucleoproteincomplexesonnascentrnatranscripts
AT josephdpuglisi realtimeassemblyofribonucleoproteincomplexesonnascentrnatranscripts
AT jamesrwilliamson realtimeassemblyofribonucleoproteincomplexesonnascentrnatranscripts
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